##gff-version 3
##sequence-region BBa_K352016 1 1923
BBa_K352016	.	engineered_region	1	980	.	+	0	ID=BBa_K352010;Name=BBa_K352010
BBa_K352016	.	promoter	1	94	.	+	0	ID=BBa_K352002;Name=BBa_K352002;Parent=BBa_K352010
BBa_K352016	.	conserved_region	36	61	.	+	0	ID=annotation2081257;Name=Protected by CooA;Parent=BBa_K352002
BBa_K352016	.	non_covalent_binding_site	41	44	.	+	0	ID=annotation2081253;Name=CooA binding site;Parent=BBa_K352002
BBa_K352016	.	conserved_region	23	91	.	+	0	ID=annotation2081258;Name=Protected by CooA+RNAP;Parent=BBa_K352002
BBa_K352016	.	non_covalent_binding_site	52	56	.	+	0	ID=annotation2081254;Name=CooA binding site;Parent=BBa_K352002
BBa_K352016	.	sequence_feature	79	84	.	+	0	ID=annotation2081255;Name=-10 Region;Parent=BBa_K352002
BBa_K352016	.	sequence_feature	8	33	.	+	0	ID=annotation2081252;Name=A+T rich sequence;Parent=BBa_K352002
BBa_K352016	.	start_codon	92	93	.	+	0	ID=annotation2081256;Name=Transcription Start;Parent=BBa_K352002
BBa_K352016	.	engineered_region	103	980	.	+	0	ID=BBa_E0840;Name=BBa_E0840;Parent=BBa_K352010
BBa_K352016	.	terminator	852	931	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K352016	.	stem_loop	863	906	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K352016	.	engineered_region	852	931	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K352016	.	ribosome_entry_site	103	117	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K352016	.	ribosome_entry_site	103	117	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K352016	.	engineered_region	103	117	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K352016	.	ribosome_entry_site	110	114	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K352016	.	terminator	940	980	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K352016	.	stem_loop	947	966	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K352016	.	stop_codon	973	973	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K352016	.	polyA_site	967	980	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K352016	.	engineered_region	940	980	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K352016	.	CDS	124	843	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K352016	.	CDS	124	843	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K352016	.	engineered_region	989	1923	.	+	0	ID=BBa_K352011;Name=BBa_K352011
BBa_K352016	.	engineered_region	1063	1923	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_K352011
BBa_K352016	.	terminator	1883	1923	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K352016	.	stem_loop	1890	1909	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K352016	.	stop_codon	1916	1916	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K352016	.	polyA_site	1910	1923	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K352016	.	engineered_region	1883	1923	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K352016	.	ribosome_entry_site	1063	1074	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K352016	.	conserved_region	1067	1070	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K352016	.	terminator	1795	1874	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K352016	.	stem_loop	1806	1849	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K352016	.	engineered_region	1795	1874	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K352016	.	CDS	1081	1761	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K352016	.	engineered_tag	1762	1786	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K352016	.	CDS	1081	1755	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K352016	.	promoter	989	1054	.	+	0	ID=BBa_K352003;Name=BBa_K352003;Parent=BBa_K352011
BBa_K352016	.	non_covalent_binding_site	1002	1006	.	+	0	ID=annotation2081259;Name=CooA binding Site;Parent=BBa_K352003
BBa_K352016	.	non_covalent_binding_site	1013	1017	.	+	0	ID=annotation2081260;Name=CooA binding Site;Parent=BBa_K352003
>BBa_K352016
ttcggcgtcttttcatacccccataaaaactctggataactgtcatctggccgacagacggggccgggctttttgtcgc
ttactcggcgccaggtactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgt
cccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaaca
tacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcg
gttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaagg
ttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgat
acccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaataca
actataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaa
cattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttacca
gacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagt
ttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagacagcatgacgagtgtcggccaagtgacggaattttccg
cccaggtggcgtactcccgttctcacttactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaa
agagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggt
cgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgt
ccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttccc
ggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaa
gacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaacca
tgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaact
gaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttac
aaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtc
gtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttata