BBa_K337031

BBa_K337031 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K337031

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R

Date created: 2010-10-21 11:00:00

Date modified: 2015-05-08 01:12:06

BGH(rc)/shRNA6(rc)/RSV(rc)/CMV_TetO2(fw)

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K337031_sequence (Version 1)

Description

RSV promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator. Those elements are all rc, CMV promoter near RSV drives in the opposite direction (fw) followed by twice Tet operator.

Notes

standard assembly

Source

see subparts

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BGH Terminator	1,255	feature/misc, sequence_feature
RSV	427,1048	promoter, feature/promoter
CMV_TetO2	1056,1784	feature/promoter, promoter
synthetic microRNA	261,421	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K337031/1

Member of these Collections

• iGEM Parts Registry