##gff-version 3
##sequence-region BBa_K336101 1 2764
BBa_K336101	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K336101	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K336101	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K336101	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K336101	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K336101	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K336101	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K336101	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K336101	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K336101	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K336101	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K336101	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K336101	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K336101	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K336101	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K336101	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K336101	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K336101	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K336101	.	engineered_region	1007	2764	.	+	0	ID=BBa_K336017;Name=BBa_K336017
BBa_K336101	.	engineered_region	2241	2764	.	+	0	ID=BBa_K336016;Name=BBa_K336016;Parent=BBa_K336017
BBa_K336101	.	ribosome_entry_site	2304	2315	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K336016
BBa_K336101	.	conserved_region	2308	2311	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K336101	.	CDS	2322	2627	.	+	0	ID=BBa_K145151;Name=BBa_K145151;Parent=BBa_K336016
BBa_K336101	.	start_codon	2322	2324	.	+	0	ID=annotation1970251;Name=start;Parent=BBa_K145151
BBa_K336101	.	CDS	2322	2624	.	+	0	ID=annotation1970253;Name=cds;Parent=BBa_K145151
BBa_K336101	.	stop_codon	2625	2627	.	+	0	ID=annotation1970252;Name=stop;Parent=BBa_K145151
BBa_K336101	.	terminator	2636	2715	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K336016
BBa_K336101	.	engineered_region	2636	2715	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K336101	.	stem_loop	2647	2690	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K336101	.	promoter	2241	2295	.	+	0	ID=BBa_K091156;Name=BBa_K091156;Parent=BBa_K336016
BBa_K336101	.	sequence_feature	2282	2287	.	+	0	ID=annotation1968421;Name=-10 Region;Parent=BBa_K091156
BBa_K336101	.	sequence_feature	2260	2265	.	+	0	ID=annotation1968420;Name=-35 Region;Parent=BBa_K091156
BBa_K336101	.	non_covalent_binding_site	2241	2260	.	+	0	ID=annotation1968422;Name=LuxR ;Parent=BBa_K091156
BBa_K336101	.	terminator	2724	2764	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K336016
BBa_K336101	.	engineered_region	2724	2764	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K336101	.	stem_loop	2731	2750	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K336101	.	polyA_site	2751	2764	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K336101	.	stop_codon	2757	2757	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K336101	.	engineered_region	1007	2232	.	+	0	ID=BBa_K336015;Name=BBa_K336015;Parent=BBa_K336017
BBa_K336101	.	terminator	2192	2232	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K336015
BBa_K336101	.	engineered_region	2192	2232	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K336101	.	stem_loop	2199	2218	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K336101	.	polyA_site	2219	2232	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K336101	.	stop_codon	2225	2225	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K336101	.	promoter	1007	1060	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K336015
BBa_K336101	.	promoter	1049	1054	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K336101	.	promoter	1026	1031	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K336101	.	engineered_region	1007	1060	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K336101	.	non_covalent_binding_site	1032	1050	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K336101	.	non_covalent_binding_site	1007	1025	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K336101	.	engineered_region	1069	2095	.	+	0	ID=BBa_K336013;Name=BBa_K336013;Parent=BBa_K336015
BBa_K336101	.	CDS	1087	2095	.	+	0	ID=BBa_K336012;Name=BBa_K336012;Parent=BBa_K336013
BBa_K336101	.	engineered_region	1087	2095	.	+	0	ID=annotation2104228;Name=BBa_K336012;Parent=BBa_K336012
BBa_K336101	.	ribosome_entry_site	1069	1080	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K336013
BBa_K336101	.	conserved_region	1073	1076	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K336101	.	terminator	2104	2183	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K336015
BBa_K336101	.	engineered_region	2104	2183	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K336101	.	stem_loop	2115	2158	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K336101	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K336101	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K336101	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K336101	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K336101	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K336101
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagatt
gacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgttcatggtgttttgatc
attttaaatttttatatggcgggtggtgggcaactcgcttgcgcgggcaactcgcttaccgattacgttagggctgata
tttacgtaaaaatcgtcaagggatgcaagaccaaagtagtaaaaccccggagtcaacagcatccaagcccaagtccttc
acggagaaaccccagcgtccacatcacgagcgaaggaccacctctaggcatcggacgcaccatccaattagaagcagca
aagcgaaacagcccaagaaaaaggtcggcccgtcggccttttctgcaacgctgatcacgggcagcgatccaaccaacac
cctccagagtgactaggggcggaaatttaaagggattaatttccactcaaccacaaatcacagtcgtccccggtattgt
cctgcagaatgcaatttaaactcttctgcgaatcgcttggattccccgcccctggccgtagagcttaaagtatgtccct
tgtcgatgcgatgtatcacaacatataaatactagcaagggatgccatgcttggaggatagcaaccgacaacatcacat
caagctctcccttctctgaacaataaaccccacagaaggcatttatgatggtcgcgtggtggtctctatttctgtacgg
ccttcaggtcgcggcacctgctttggctgcaacgcctgcggactggcgatcgcaatccatttatttccttctcacggat
cgatttgcaaggacggatgggtcgacgactgcgacttgtaatactgcggatcaggtgtgttgttacctactagctttca
gaaagaggaatgtaaactgacttgatatagaaatactgtggtggaacatggcagggcatcatcgacaaggtaaattgcc
cctttatcaaaaaaaaagaaggaaaagcagaagaaaaataaaataaaaagaactctagtcctaaccatcacatagttgg
actatatccagggaatgggcttcacagccatctggtaataatactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttaatcaagaaaatggtttgttatagtcgaa
taaatactagagaaagaggagaaatactagatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgt
ttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtc
agataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggcc
agtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacc
tgatgttctggggaatataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata