Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: IC 2010 Team
Date created: 2010-10-20 11:00:00
Date modified: 2015-05-08 01:11:56
N-terminus his tagged-GFP-XylE fusion protein
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K316006_sequence (Version 1) |
Description
Constructed to be combined with promoter and terminator. The GFP is linked to XylE monomer subunit by a GGGSGGGS linker with the aim to render the enzyme inactive, via preventing tetramerization (it???s functional form).Notes
Standard biobrick assembly and PCR primer extension. For full methods please see our wiki http://2010.igem.org/Team:Imperial_College_London/StrategySource
Existing biobrick parts with modifications using PCR primer extension| Sequence Annotation | Location | Component / Role(s) |
| His tag Start GFP E0040 Flag Tag Tev Protease Cleavage Site GGS linker XylE J33204 | 4,21 1,3 22,732 733,756 757,777 778,795 796,1722 | feature/tag tag start_codon feature/start CDS feature/protein tag feature/tag feature/misc sequence_feature feature/misc sequence_feature CDS feature/protein |