##gff-version 3
##sequence-region BBa_K255002 1 3037
BBa_K255002	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K255002	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K255002	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K255002	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K255002	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K255002	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K255002	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K255002	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K255002	.	ribosome_entry_site	2160	2171	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K255002	.	conserved_region	2164	2167	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K255002	.	promoter	2098	2151	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K255002	.	promoter	2117	2122	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K255002	.	non_covalent_binding_site	2098	2116	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K255002	.	engineered_region	2098	2151	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K255002	.	promoter	2140	2145	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K255002	.	non_covalent_binding_site	2123	2141	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K255002	.	terminator	2049	2089	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K255002	.	stem_loop	2056	2075	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K255002	.	engineered_region	2049	2089	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K255002	.	stop_codon	2082	2082	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K255002	.	polyA_site	2076	2089	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K255002	.	CDS	2178	2900	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_K255002	.	terminator	1961	2040	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K255002	.	engineered_region	1961	2040	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K255002	.	stem_loop	1972	2015	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K255002	.	terminator	2997	3037	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K255002	.	stem_loop	3004	3023	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K255002	.	engineered_region	2997	3037	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K255002	.	stop_codon	3030	3030	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K255002	.	polyA_site	3024	3037	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K255002	.	CDS	81	1952	.	+	0	ID=BBa_K255000;Name=BBa_K255000
BBa_K255002	.	terminator	2909	2988	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K255002	.	engineered_region	2909	2988	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K255002	.	stem_loop	2920	2963	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K255002
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccag
gccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtgg
cacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaat
tgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaa
gcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccagg
atgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacag
tattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctg
ttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattc
agccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcat
cgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgc
gttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatca
aacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaa
tcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttg
gccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagctcgagggagaaggacaaggacaag
gacaaggaccaggacgaggatacgcataccgaagcggttccggaatggtgagcaagggcgaggagctgttcaccggggt
ggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgcc
acctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccc
tgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccga
aggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggc
gacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagt
acaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgcca
caacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctg
cccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctgg
agttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagt
gatagagatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggt
ggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgcc
acctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccacct
tcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccga
aggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggc
gacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagt
acaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgcca
caacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctg
cccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctgg
agttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttata