##gff-version 3
##sequence-region BBa_K238022 1 4421
BBa_K238022	.	terminator	4293	4421	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K238022	.	terminator	4293	4372	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K238022	.	engineered_region	4293	4372	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K238022	.	stem_loop	4304	4347	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K238022	.	terminator	4381	4421	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K238022	.	engineered_region	4381	4421	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K238022	.	stop_codon	4414	4414	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K238022	.	stem_loop	4388	4407	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K238022	.	polyA_site	4408	4421	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K238022	.	engineered_region	1	4284	.	+	0	ID=BBa_K238021;Name=BBa_K238021
BBa_K238022	.	engineered_region	3168	4284	.	+	0	ID=BBa_I742109;Name=BBa_I742109;Parent=BBa_K238021
BBa_K238022	.	CDS	3184	4284	.	+	0	ID=BBa_I742107;Name=BBa_I742107;Parent=BBa_I742109
BBa_K238022	.	CDS	3184	4284	.	+	0	ID=annotation1949817;Name=COMT coding region;Parent=BBa_I742107
BBa_K238022	.	ribosome_entry_site	3168	3177	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_I742109
BBa_K238022	.	sequence_feature	3168	3170	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K238022	.	ribosome_entry_site	3171	3177	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K238022	.	engineered_region	1	3159	.	+	0	ID=BBa_K238020;Name=BBa_K238020;Parent=BBa_K238021
BBa_K238022	.	engineered_region	44	3159	.	+	0	ID=BBa_K238019;Name=BBa_K238019;Parent=BBa_K238020
BBa_K238022	.	engineered_region	44	1593	.	+	0	ID=BBa_K238027;Name=BBa_K238027;Parent=BBa_K238019
BBa_K238022	.	ribosome_entry_site	44	57	.	+	0	ID=annotation2028781;Name=rbs;Parent=BBa_K238027
BBa_K238022	.	CDS	58	1593	.	+	0	ID=annotation2028782;Name=sam8;Parent=BBa_K238027
BBa_K238022	.	engineered_region	1602	3159	.	+	0	ID=BBa_K238007;Name=BBa_K238007;Parent=BBa_K238019
BBa_K238022	.	CDS	1618	3159	.	+	0	ID=annotation2013241;Name=sam5;Parent=BBa_K238007
BBa_K238022	.	ribosome_entry_site	1602	1617	.	+	0	ID=annotation2013240;Name=rbs;Parent=BBa_K238007
BBa_K238022	.	promoter	1	35	.	+	0	ID=BBa_J23110;Name=BBa_J23110;Parent=BBa_K238020
>BBa_K238022
tttacggctagctcagtcctaggtacaatgctagctactagaggaggttgggacaagatgacgcaggtcgtggaacgtc
aggctgatcggctcagcagcagggagtacctggcccgggtcgtgcgcagcgccgggtgggacgccggtctcacctcgtg
caccgacgaggagatcgtccggatgggcgcgagcgcgcgcaccatcgaggagtacctgaagtccgacaagcccatctac
ggcctgacgcagggcttcggtccgctggtgctgttcgacgccgactcggagctggagcagggcggctcgctgatctcgc
acctgggcaccggccagggcgcgccactggccccggaggtgtcgcggctgatcctctggctgcgcatccagaacatgcg
caaggggtactcggcggtctcgccggtgttctggcagaagctcgccgacctgtggaacaaggggttcaccccggcgatc
ccccggcacggcacggtcagcgcgagcggcgacctgcaaccgctggcgcacgccgcgctcgccttcaccggtgtcggcg
aggcgtggacccgggacgccgacggccggtggtccaccgtgccggccgtggacgcgctcgccgcgctgggggcggagcc
gttcgactggccggtgcgcgaggcgctggcgttcgtcaacgggaccggcgcgagcctcgcggtggctgtgctcaaccac
cggtccgccctgcggctggtccgcgcctgcgccgtgctctccgcgcggctggcgaccctgctgggggccaatcccgagc
actacgacgtggggcacggtgtcgcgcgcggccaggtcggtcagctgaccgcggcggagtggatccggcaggggctgcc
ccggggcatggtgcgcgacggcagccgcccgctccaggagccgtacagcctgcggtgcgcgccgcaggtgctcggcgcg
gtgctcgaccagctcgacggcgcgggcgacgtgctggcgcgggaggtcgacggctgccaggacaacccgatcacctacg
agggcgagctgctgcacggcggcaacttccacgccatgccggtgggtttcgcctccgaccagatcgggttggccatgca
catggccgcctacctggccgagcgccagctgggtctgctggtcagcccggtgaccaacggcgacctgccgcccatgctc
accccgcgcgccgggcgcggtgccgggctggccggggtgcagatcagcgcgacctcgttcgtctcgcggatccggcagc
tggtgttccccgcctcgctgaccaccctgccgaccaacggctggaaccaggaccacgtgccgatggcgctcaacggggc
gaactcggtgttcgaggcgttggagctcggctggctgacggtcgggtcgctggcggtgggcgtcgcgcagctcgcggcc
atgaccggccacgccgcggagggcgtctgggcggagctggccgggatctgcccgccgctggacgccgaccgcccgctgg
gcgccgaggtgcgcgccgcgcgcgacctgctgtccgcgcacgcggaccaactgctcgtcgacgaggcagacgggaagga
tttcggataataatactagagacggagaagcagcgaaatgaccatcacgtcacctgcgccggcgggccggctcaacaac
gtccgcccgatgacgggcgaggagtacctggaatcactgcgggacggccgagaggtctacatctacggcgagcgggtcg
acgacgtcaccacgcacctggccttccgcaacagcgtgcgctccatcgcgcggctctacgacgtgctgcacgacccggc
gtccgaaggtgtgctgcgggtgcccaccgacaccggcaacggcgggttcacccacccgttcttcaagaccgcccggtcg
tcggaggacctggtcgccgcgcgcgaggccatcgtcggctggcagcggctggtgtacgggtggatgggccgcaccccgg
actacaaggcggcgttcttcggcacgctcgacgccaacgccgagttctacgggccgttcgaggccaacgcccgccgctg
gtaccgcgacgcccaggaacgggtgctgtacttcaaccacgcgatcgtgcacccgccggtcgaccgggaccggcccgcc
gaccggaccgccgacatctgcgtgcacgtggaggaggagaccgacagcgggttgatcgtctccggcgccaaggtggtcg
cgaccggctccgcgatgaccaacgcgaacctcatcgcgcactacgggcttccggtgcgggacaagaagttcggcctggt
gttcacggtcccgatgaactcgcccggcctcaagctcatctgccgcacctcctacgagctgatggtcgcgacgcagggc
tcgcccttcgactacccgctgtcgagccggctcgacgagaacgactcgatcatgatcttcgaccgggtgctggtgccct
gggagaacgtgttcatgtacgacgcgggcgcggccaactccttcgccaccgggtcaggcttcctcgaacgcttcacctt
ccacggctgcacccgcctcgcggtcaagctggacttcatcgccggctgcgtcatgaaggcggtggaggtcaccggcacc
acgcacttccggggcgtgcaggcgcaggtcggcgaagtgctcaactggcgcgacgtgttctggggcctgtccgacgcga
tggccaagtcgccgaactcgtgggtcggcggctcggtgcagccgaacctcaactacgggctcgcgtaccgcaccttcat
gggcgtgggctacccgcgcatcaaggagatcatccagcagaccctcggcagcgggttgatctacctgaactcctcggcc
gccgactggaagaaccccgacgtccgcccgtacctcgaccgctacctgcgcggctcgcggggcatccaggcgatcgacc
gggtcaagctgctgaagctgctgtgggacgcggtcggcaccgagttcgccggccggcacgagctctacgagcgcaacta
cggcggcgaccacgagggcatccgggtgcagaccctgcaagcgtaccaggcgaacggccaagccgccgcgctcaagggt
ttcgccgagcagtgcatgtccgagtacgacctcgacggctggacgaggcccgacctgatcaaccccggcacctaataat
actagagctcaaggaggtactagatgggttcaacaggtgaaactcaaataacaccaacccacatatcagatgaagaagc
aaacctcttcgccatgcaactagcaagtgcttcagttcttcccatgattttgaaatcagctcttgaacttgatctctta
gaaatcattgctaaagctggacctggtgctcaaatttcacctattgaaattgcttctcagctaccaacaactaaccctg
atgcaccagttatgttggaccgaatgttgcgtctcttggcttgttacataatcctcacatgttcagttcgtactcaaca
agatggaaaggttcagagactttatggtttggctactgttgctaagtatttggttaagaatgaagatggtgtatccatt
tctgctcttaatctcatgaatcaggataaagtgctcatggaaagctggtaccacctaaaagatgcagtccttgatgggg
gcattccattcaacaaggcttatggaatgacagcctttgaataccatggaacagatccaaggtttaacaaggttttcaa
caaggggatgtctgatcactctaccatcacaatgaagaaaattcttgagacctacacaggttttgaaggccttaaatct
cttgttgatgtaggtggtggtactggagctgtaattaacacgattgtctcaaaatatcccactataaagggtataaatt
ttgatttaccccatgtcattgaagatgctccatcttatccaggagttgagcatgttggtggagacatgtttgtcagtat
tccaaaggctgatgctgtttttatgaagtggatttgtcatgactggagtgatgagcactgcttgaaatttttgaagaac
tgctatgaggcactgccagacaatggaaaagtgattgtggcagaatgcatacttccagtggctccagattcaagcctgg
ccacaaaaggtgtggttcacattgatgtgatcatgttggctcataatcctggtgggaaagagagaacacaaaaagagtt
tgaggatcttgccaaaggtgctggattccaaggtttcaaagtccattgtaatgctttcaacacatacatcatggagttt
cttaagaaggtttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata