Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lin Min
Date created: 2009-09-21 11:00:00
Date modified: 2015-05-08 01:11:34
pSal-rbs-PhiR73 ( amber mutation )
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K228865_sequence (Version 1) |
Description
T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.Notes
when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.Source
part registery 2009 distribution & PKU 2009 igem team| Access | Instance | Definition |
| public public public | BBa_K228004 BBa_B0034 BBa_K228863 | BBa_K228004 BBa_B0034 BBa_K228863 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K228004 BBa_B0034 BBa_K228863 | 1,1052 1061,1072 1079,1321 | BBa_K228004 BBa_B0034 BBa_K228863 |