##gff-version 3
##sequence-region BBa_K228108 1 3035
BBa_K228108	.	CDS	226	2898	.	+	0	ID=BBa_K228000;Name=BBa_K228000
BBa_K228108	.	CDS	226	2898	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K228108	.	terminator	2907	2986	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228108	.	stem_loop	2918	2961	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228108	.	engineered_region	2907	2986	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228108	.	ribosome_entry_site	209	219	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K228108	.	ribosome_entry_site	209	219	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K228108	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K228108	.	engineered_region	209	219	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K228108	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K228108	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K228108	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K228108	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K228108	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K228108	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K228108	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K228108	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K228108	.	terminator	2995	3035	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228108	.	stem_loop	3002	3021	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228108	.	polyA_site	3022	3035	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228108	.	stop_codon	3028	3028	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228108	.	engineered_region	2995	3035	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K228108
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggactactagatgaccatgatt
accgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactc
tggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacg
cttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactacc
ctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagcct
tccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgc
tgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgt
gaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcat
ttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaaga
ctctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgct
ggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgc
tggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattg
ggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatg
cctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacg
taatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaaga
catcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaag
tctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttaca
acatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttac
gctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgat
aaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactgg
agaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcacca
cggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctc
cgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaaga
aagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactgg
tgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtg
actaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattc
agccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttg
ggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgct
gaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctg
tgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccat
taacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagac
ggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactcct
tcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtga
tgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggct
aaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcataatactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttata