##gff-version 3
##sequence-region BBa_K2150021 1 4537
BBa_K2150021	.	terminator	755	883	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2150021	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2150021	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2150021	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2150021	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2150021	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2150021	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2150021	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2150021	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2150021	.	CDS	1746	4400	.	+	0	ID=BBa_I2032;Name=BBa_I2032
BBa_K2150021	.	promoter	4243	4270	.	+	0	ID=annotation1928674;Name=putative E. coli promoter;Parent=BBa_I2032
BBa_K2150021	.	CDS	62	746	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K2150021	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K2150021	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K2150021	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K2150021	.	terminator	4409	4537	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2150021	.	terminator	4497	4537	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2150021	.	polyA_site	4524	4537	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2150021	.	engineered_region	4497	4537	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2150021	.	stop_codon	4530	4530	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2150021	.	stem_loop	4504	4523	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2150021	.	terminator	4409	4488	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2150021	.	stem_loop	4420	4463	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2150021	.	engineered_region	4409	4488	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2150021	.	promoter	892	911	.	+	0	ID=BBa_K2150031;Name=BBa_K2150031
BBa_K2150021	.	CDS	1000	1719	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K2150021	.	CDS	1000	1719	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K2150021	.	ribosome_entry_site	982	993	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2150021	.	conserved_region	986	989	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2150021	.	promoter	1	35	.	+	0	ID=BBa_J23119;Name=BBa_J23119
BBa_K2150021	.	ribosome_entry_site	1728	1739	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2150021	.	conserved_region	1732	1735	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2150021	.	promoter	920	973	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K2150021	.	non_covalent_binding_site	920	938	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K2150021	.	non_covalent_binding_site	945	963	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K2150021	.	promoter	962	967	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K2150021	.	promoter	939	944	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K2150021	.	engineered_region	920	973	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K2150021	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2150021	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K2150021
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtaatacgactcactatagggtactagagtccctatcagtgatagagattgacatccc
tatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactg
gagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtga
tgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcact
actttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgc
ccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttga
aggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattg
gaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatta
gacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcct
tttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcctt
cttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagaaagaggagaa
atactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctg
gctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgct
tccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccct
actccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttc
cagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctg
acaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtga
ccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcattt
atgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagact
ctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctgg
cgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctg
gctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattggg
ctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcc
tgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgta
atcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagaca
tcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtc
tcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaac
atggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgc
tggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataa
ggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggag
aacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacg
gcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccg
agatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaa
gtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtg
aaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgac
taagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcag
ccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttggg
aatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctga
ggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtg
tggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccatta
acaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacgg
tagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttc
ggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatg
tactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaa
aggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgtaataatactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttata