##gff-version 3
##sequence-region BBa_K2069005 1 2221
BBa_K2069005	.	engineered_region	1	1889	.	+	0	ID=BBa_K2069000;Name=BBa_K2069000
BBa_K2069005	.	engineered_region	1677	1889	.	+	0	ID=BBa_K538104;Name=BBa_K538104;Parent=BBa_K2069000
BBa_K2069005	.	CDS	1695	1889	.	+	0	ID=BBa_K538004;Name=BBa_K538004;Parent=BBa_K538104
BBa_K2069005	.	non_covalent_binding_site	1773	1787	.	+	0	ID=annotation2129606;Name=RNA binding motif (RPN2);Parent=BBa_K538004
BBa_K2069005	.	start_codon	1695	1697	.	+	0	ID=annotation2127783;Name=Start Codon;Parent=BBa_K538004
BBa_K2069005	.	point_mutation	1769	1769	.	+	0	ID=annotation2127785;Name=A > G (Recode for SapI site);Parent=BBa_K538004
BBa_K2069005	.	stop_codon	1884	1889	.	+	0	ID=annotation2127784;Name=Double Stop Codon;Parent=BBa_K538004
BBa_K2069005	.	non_covalent_binding_site	1731	1748	.	+	0	ID=annotation2129605;Name=RNA binding motif (RPN1);Parent=BBa_K538004
BBa_K2069005	.	ribosome_entry_site	1677	1688	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K538104
BBa_K2069005	.	conserved_region	1681	1684	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2069005	.	engineered_region	1	1668	.	+	0	ID=BBa_K538101;Name=BBa_K538101;Parent=BBa_K2069000
BBa_K2069005	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K538101
BBa_K2069005	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2069005	.	CDS	19	1668	.	+	0	ID=BBa_K538001;Name=BBa_K538001;Parent=BBa_K538101
BBa_K2069005	.	start_codon	19	21	.	+	0	ID=annotation2124231;Name=Start Codon;Parent=BBa_K538001
BBa_K2069005	.	stop_codon	1663	1668	.	+	0	ID=annotation2124232;Name=Double Stop Codon;Parent=BBa_K538001
BBa_K2069005	.	point_mutation	926	926	.	+	0	ID=annotation2128866;Name=A > G (Recode for SapI site);Parent=BBa_K538001
BBa_K2069005	.	engineered_region	1898	2221	.	+	0	ID=BBa_K1364007;Name=BBa_K1364007
BBa_K2069005	.	terminator	2093	2221	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1364007
BBa_K2069005	.	terminator	2181	2221	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2069005	.	stop_codon	2214	2214	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2069005	.	polyA_site	2208	2221	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2069005	.	stem_loop	2188	2207	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2069005	.	engineered_region	2181	2221	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2069005	.	terminator	2093	2172	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2069005	.	stem_loop	2104	2147	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2069005	.	engineered_region	2093	2172	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2069005	.	engineered_region	1898	2084	.	+	0	ID=BBa_K1364002;Name=BBa_K1364002;Parent=BBa_K1364007
BBa_K2069005	.	ribosome_entry_site	1898	1913	.	+	0	ID=annotation2391440;Name=strong RBS K780002;Parent=BBa_K1364002
BBa_K2069005	.	CDS	2037	2084	.	+	0	ID=annotation2391443;Name=GAFP-1;Parent=BBa_K1364002
BBa_K2069005	.	CDS	1914	1994	.	+	0	ID=annotation2391441;Name=Signal peptide;Parent=BBa_K1364002
BBa_K2069005	.	CDS	1995	2036	.	+	0	ID=annotation2391442;Name=Propeptide;Parent=BBa_K1364002
>BBa_K2069005
aaagaggagaaatactagatggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaa
acattttagccgacgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcacc
gatcatcaccaaagatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatg
gttaaggaagttgcttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgatta
tcagcgaaggcttgaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgc
tgttgttgccgccattaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgcc
aatgccgatgaaacggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaag
aaggcaaaggccttgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcat
caacaaccaagaaaaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaa
gagctgttgccaattcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaag
cactagcaacattggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcg
tcgtaaagcgatgttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaa
actgcggatccttcttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcag
gtactgaagcaagcgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcga
aaagttacaagaacgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatg
aaagagaagaaagaccgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtg
gtgttgctttgattcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggc
acttcgtgcgatggaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtg
aaatctggcacaggtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagacc
ctgcaaaagtcacgcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgc
ggatgcgcctgttgaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatg
atgtaataatactagagaaagaggagaaatactagatgaataaaggtaccgtaaaatttttcaatgaatctaaaggatt
cggatttatcactgaagaaggaaacaatgaggagcattttgtgcacgtgtcaggattagtagacgaaattcgtgaaaac
gatgaggttgaatttgacttacaagatggaagaaaaggattaaacgcagtaaacgtaagagttatataataatactaga
gagagaacaaggaggggatgtttgcaaagagatttaagacgagtttacttccgctgtttgccggcttccttttgctgtt
tcaccttgtattagccggtcctgcggcagcaagtgcggaaacggctaacaaaagtaacgagctggatagcctttccttt
tcatataataatttcgaagaggacgattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttata