##gff-version 3
##sequence-region BBa_K2032007 1 3525
BBa_K2032007	.	engineered_region	1	2448	.	+	0	ID=BBa_K2032002;Name=BBa_K2032002
BBa_K2032007	.	ribosome_entry_site	1257	1269	.	+	0	ID=annotation2515952;Name=BBa_B0032;Parent=BBa_K2032002
BBa_K2032007	.	ribosome_entry_site	2352	2364	.	+	0	ID=annotation2515953;Name=BBa_B0032;Parent=BBa_K2032002
BBa_K2032007	.	engineered_region	3513	3592	.	+	0	ID=annotation2515950;Name=BBa_B0010;Parent=BBa_K2032002
BBa_K2032007	.	engineered_region	1276	2340	.	+	0	ID=annotation2515955;Name=BBa_K1537025;Parent=BBa_K2032002
BBa_K2032007	.	engineered_region	3601	3641	.	+	0	ID=annotation2515951;Name=BBa_B0012;Parent=BBa_K2032002
BBa_K2032007	.	engineered_region	2371	3501	.	+	0	ID=annotation2515954;Name=BBa_K1537026;Parent=BBa_K2032002
BBa_K2032007	.	engineered_region	1	1247	.	+	0	ID=annotation2515949;Name=BBa_K2032003;Parent=BBa_K2032002
BBa_K2032007	.	engineered_region	2457	3525	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_K2032007	.	ribosome_entry_site	2665	2676	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K2032007	.	conserved_region	2669	2672	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2032007	.	terminator	3397	3476	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K2032007	.	engineered_region	3397	3476	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2032007	.	stem_loop	3408	3451	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2032007	.	terminator	3485	3525	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K2032007	.	engineered_region	3485	3525	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2032007	.	stop_codon	3518	3518	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2032007	.	polyA_site	3512	3525	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2032007	.	stem_loop	3492	3511	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2032007	.	CDS	2683	3363	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K2032007	.	CDS	2683	3357	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K2032007	.	engineered_tag	3364	3388	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K2032007	.	promoter	2457	2656	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K2032007	.	CDS	2457	2544	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K2032007	.	non_covalent_binding_site	2545	2582	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K2032007	.	start_codon	2629	2629	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K2032007	.	engineered_region	2457	2656	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K2032007	.	promoter	2617	2622	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K2032007	.	non_covalent_binding_site	2622	2656	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K2032007	.	promoter	2593	2598	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K2032007	.	terminator	2402	2442	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K2032007	.	engineered_region	2402	2442	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2032007	.	stop_codon	2435	2435	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2032007	.	polyA_site	2429	2442	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2032007	.	stem_loop	2409	2428	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2032007	.	terminator	2314	2393	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K2032007	.	engineered_region	2314	2393	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2032007	.	stem_loop	2325	2368	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K2032007
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtac
tagatgtcttcaggagatagtaagaaaattgttggtgtgttttataaggctaacgagtacgcaactaaaaatccaaact
tccttggatgtgttgagaatgctcttggtattagagattggttggaatcacaaggacatcagtatatcgttacagatga
taaggagggtccagattgcgaacttgagaaacatattcctgatctccacgttttaatctctacaccatttcatcctgct
tacgtgaccgcagaaaggattaagaaagctaagaacttgaaacttttgctcaccgcaggaatcggttcagatcacattg
atcttcaagctgctgctgctgctggacttactgttgctgaagtgactggtagtaacgttgtgtctgttgcagaagatga
gttgatgagaattttgattcttatgaggaacttcgttcctggatataaccaagttgtgaagggagagtggaatgtggca
ggtatcgcttatagagcatacgatcttgaaggaaaaaccataggtactgttggagctggtagaattggaaagttacttt
tgcagaggttgaaaccatttggttgtaatctcttataccatgataggctccaaatggctcctgaattagagaaggaaac
aggtgcaaaattcgttgaggatcttaatgagatgttgccaaagtgcgatgttatagtgattaacatgcctctcacagag
aaaaccagaggaatgttcaataaggaactcataggaaaattaaagaaaggtgttttgatcgtgaataacgctagaggag
ctattatggaaaggcaggctgttgtggatgcagttgaatcaggacacatcggaggttatagtggagatgtttgggaccc
tcaaccagctcctaaggatcatccatggagatacatgcctaaccaggctatgactccacacacatctggaactacaatc
gatgctcaactcagatatgcagctggaactaaggatatgttagagaggtacttcaaaggtgaagattttccaaccgaga
attacatagtgaaggatggtgaacttgctccacaatacaggtactaatagagtcacacaggaaagtactagatgggagc
agtgacttaccaaggaaaaaattctatcgctgttaagaaagtggatgcaccatcaattcaggatagagaggatgttatt
atcaggatcacttctacagctatatgtggatctgatcttcatctttatcagggtaacttccctttgccaataggatacg
ttattggtcacgaacctatgggaattgttgaagaagtgggtccagatgttacagctgtgaagaaaggagatagagttgt
gatcccttttaccgttgcatgtggtaaatgccaatattgtgatcatcatcttgaatcacagtgcgataatagtaaccca
cattatgatagtggaggtttattcggatactctgagaagtatggtaattaccctggaggtcaagctgaatatctcaggg
ttccatttggaaactacacacctttcaaaattccagatgattgcgaattggaggatgaacagcttttgtttctctctga
tgtgttacctaccgcttactggtctgttgagcatgcaggagtgaagaaaggagatacagttatcgtgctcggatgtggt
ccagttggacttatggctcaacagttcgcatggcaaaagggtgctgaaagagttattgcagtggattatatcgattaca
gacttaggcacgctaaaaggatgaatggtgttgaggtgtttgatttcactgaagatcctgatatgggtgagacacttaa
agaattgaccaaaggaggtgcagatgttgtgattgattgtgttggtatggatggaaagaagtctcctttggagaagatc
gaacaaaaacttaaacttcaaggaggtactataggtccaatccaaatagctacaaaggcagttagaaaaaggggaaccg
ttcagatgactggtgtgtatggaggtctttacaatatgtttcctcttggagctttctttgcaagaaacgttacacttaa
aatgggacaagctccagcaaggggttatatgtcaaagttgtaccagaaagtgaccagtggagagattgatcctagagct
ataattactcacaaactcccattagatgaagctgcacacgcataccatatcttcaacgataaacaggatgattgcataa
aagtgattctcaagccttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
actagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgact
ggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgct
tccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgg
cttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcga
aatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctg
ccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccgg
actacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgt
tacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggt
ccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtg
aaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaacc
ggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaa
cagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttata