##gff-version 3
##sequence-region BBa_K1972014 1 7367
BBa_K1972014	.	CDS	1316	3085	.	+	0	ID=BBa_K1972005;Name=BBa_K1972005
BBa_K1972014	.	CDS	1388	2485	.	+	0	ID=annotation2500556;Name=dszB-optimized;Parent=BBa_K1972005
BBa_K1972014	.	ribosome_entry_site	1369	1387	.	+	0	ID=annotation2500555;Name=RBS;Parent=BBa_K1972005
BBa_K1972014	.	promoter	1316	1368	.	+	0	ID=annotation2500554;Name=tac promoter;Parent=BBa_K1972005
BBa_K1972014	.	CDS	1	1307	.	+	0	ID=BBa_K1972008;Name=BBa_K1972008
BBa_K1972014	.	terminator	1179	1307	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1972008
BBa_K1972014	.	terminator	1267	1307	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1972014	.	stop_codon	1300	1300	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1972014	.	polyA_site	1294	1307	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1972014	.	stem_loop	1274	1293	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1972014	.	engineered_region	1267	1307	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1972014	.	terminator	1179	1258	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1972014	.	engineered_region	1179	1258	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1972014	.	stem_loop	1190	1233	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1972014	.	CDS	1	1170	.	+	0	ID=BBa_K1972005;Name=BBa_K1972005;Parent=BBa_K1972008
BBa_K1972014	.	CDS	73	1170	.	+	0	ID=annotation2500556;Name=dszB-optimized;Parent=BBa_K1972005
BBa_K1972014	.	ribosome_entry_site	54	72	.	+	0	ID=annotation2500555;Name=RBS;Parent=BBa_K1972005
BBa_K1972014	.	promoter	1	53	.	+	0	ID=annotation2500554;Name=tac promoter;Parent=BBa_K1972005
BBa_K1972014	.	CDS	1316	7367	.	+	0	ID=BBa_K1972013;Name=BBa_K1972013
BBa_K1972014	.	engineered_region	3094	3876	.	+	0	ID=annotation2506008;Name=BBa_K1972004;Parent=BBa_K1972013
BBa_K1972014	.	sequence_feature	7239	7367	.	+	0	ID=annotation2506138;Name=double teminator;Parent=BBa_K1972013
BBa_K1972014	.	engineered_region	1316	3085	.	+	0	ID=annotation2505940;Name=BBa_K1972005;Parent=BBa_K1972013
BBa_K1972014	.	sequence_feature	5165	5293	.	+	0	ID=annotation2506076;Name=double teminator;Parent=BBa_K1972013
BBa_K1972014	.	engineered_region	3885	5156	.	+	0	ID=annotation2506018;Name=	BBa_K1972006;Parent=BBa_K1972013
BBa_K1972014	.	engineered_region	5337	5932	.	+	0	ID=annotation2506092;Name=BBa_K1972003;Parent=BBa_K1972013
BBa_K1972014	.	promoter	6070	6147	.	+	0	ID=annotation2506103;Name=LacI promoter;Parent=BBa_K1972013
BBa_K1972014	.	sequence_feature	5933	6061	.	+	0	ID=annotation2506101;Name=double teminator;Parent=BBa_K1972013
BBa_K1972014	.	CDS	6271	7234	.	+	0	ID=annotation2506104;Name=LacI;Parent=BBa_K1972013
BBa_K1972014	.	promoter	5294	5336	.	+	0	ID=annotation2506060;Name=LacUV5 promoter;Parent=BBa_K1972013
>BBa_K1972014
ttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaaaactgcaaggacaaccttcatgacct
ctcgcgttgatccggccaatccgggtagcgaactggatagtgccattcgcgataccttaacctatagtaattgtccggt
tccgaatgcactgttaaccgcctctgaaagcggctttctggatgcagcaggtattgaactggatgtgctgagcggccag
cagggtaccgttcattttacctatgatcagccggcatatacccgctttggcggtgaaattccgccgctgctgagtgaag
gcctgcgtgcccctggtcgtacccgcttactgggtattaccccgttactgggtcgccagggcttcttcgtgcgcgatga
tagtccgattaccgcagccgcagacctcgcaggtcgtcgtattggtgtgagcgcatcagccattcgtattctgcgcggt
cagctcggcgactatctggaattagatccgtggcgtcagaccttagttgcattaggctcatgggaagcacgtgccttat
tacataccctggaacatggcgaattaggcgttgatgatgttgaactggttccgatttcttcaccgggtgtggatgttcc
ggccgaacagttagaagaaagtgcaaccgttaaaggtgccgatctgtttccggatgttgcacgcggtcaggcagccgtg
ctggcaagtggcgatgtggatgccctgtattcttggctgccgtgggcaggcgaattacaggcaaccggcgcacgtccgg
ttgtggacctcggtttagatgaacgtaatgcatatgcaagtgtttggaccgtgtctagcggtttagttcgccagagacc
tggcctggttcagcgtctggttgatgccgccgttgatgcaggcttatgggcacgcgatcattcagatgccgtgacctca
ctgcatgccgcaaatctgggtgtgtctaccggcgcagttggtcagggctttggcgcagattttcagcagcgtctggttc
cgcgtttagatcatgatgccttagccctgttagaacgcacccagcagtttctgctgaccaacaacctgttacaggaacc
ggtggccttagatcagtgggccgccccggaatttttaaacaacagcttaaatcgtcatcgctaatactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacaattaatcatcggctcgtataat
gtgtggaattgtgagcggataacaaaactgcaaggacaaccttcatgacctctcgcgttgatccggccaatccgggtag
cgaactggatagtgccattcgcgataccttaacctatagtaattgtccggttccgaatgcactgttaaccgcctctgaa
agcggctttctggatgcagcaggtattgaactggatgtgctgagcggccagcagggtaccgttcattttacctatgatc
agccggcatatacccgctttggcggtgaaattccgccgctgctgagtgaaggcctgcgtgcccctggtcgtacccgctt
actgggtattaccccgttactgggtcgccagggcttcttcgtgcgcgatgatagtccgattaccgcagccgcagacctc
gcaggtcgtcgtattggtgtgagcgcatcagccattcgtattctgcgcggtcagctcggcgactatctggaattagatc
cgtggcgtcagaccttagttgcattaggctcatgggaagcacgtgccttattacataccctggaacatggcgaattagg
cgttgatgatgttgaactggttccgatttcttcaccgggtgtggatgttccggccgaacagttagaagaaagtgcaacc
gttaaaggtgccgatctgtttccggatgttgcacgcggtcaggcagccgtgctggcaagtggcgatgtggatgccctgt
attcttggctgccgtgggcaggcgaattacaggcaaccggcgcacgtccggttgtggacctcggtttagatgaacgtaa
tgcatatgcaagtgtttggaccgtgtctagcggtttagttcgccagagacctggcctggttcagcgtctggttgatgcc
gccgttgatgcaggcttatgggcacgcgatcattcagatgccgtgacctcactgcatgccgcaaatctgggtgtgtcta
ccggcgcagttggtcagggctttggcgcagattttcagcagcgtctggttccgcgtttagatcatgatgccttagccct
gttagaacgcacccagcagtttctgctgaccaacaacctgttacaggaaccggtggccttagatcagtgggccgccccg
gaatttttaaacaacagcttaaatcgtcatcgctaatactagagcaactgcaaggacaaccttcaatgacccagcagcg
ccagatgcaccttgcaggctttttcagcgcaggcaatgtgacccatgcacatggtgcatggcgtcataccgatgcctct
aatgattttctgagcggtaaatattatcagcatattgcacgtaccttagaacgcggcaaatttgatctgctgtttctgc
cggatggtttagccgttgaagatagctatggagacaatctggataccggcgtgggcttaggtggtcagggtgcagttgc
cttagaaccggcaagcgttgtggcaaccatggccgcagtgaccgaacacctgggtttaggtgcaaccattagcgccacc
tattatccgccgtatcatgtggcacgtgtgtttgccaccttagatcagctgagcggcggtcgtgtgtcttggaatgttg
tgacctcactgaatgatgcagaagcacgtaattttggtattaatcagcatctggaacatgatgcacgctatgatcgcgc
cgatgaatttctggaagccgtgaaaaaactgtggaactcttgggatgaagatgccctggtgctggataaagcagccggc
gtgtttgccgatccggccaaagttcattatgttgatcatcatggcgaatggttaaatgtgcgcggcccgttacaggttc
cgcgtagtccgcagggcgaaccggttattttacaggcaggcctgtctccgcgcggtcgtcgctttgccggtaaatgggc
agaagcagtgttttcactggccccgaatctggaagttatgcaggcaacctatcagggtattaaagcagaagttgatgcc
gcaggtcgcgatccggatcagaccaaaatttttaccgccgttatgccggtgctgggcgaaagtcaggcagtggcccagg
aacgcttagaatatttgaactcactggttcatccggaagttggcctgtctaccctgagtagtcataccggtattaatct
ggcagcctatccgttagataccccgattaaagatattctgcgcgacttgcaggatcgtaatgttccgacccagctgcat
atgtttgcagccgccacccatagtgaagaactgaccttagccgaaatgggtcgtcgctatggtaccaatgtgggctttg
ttccgcagtgggccggtaccggcgaacagattgccgatgaactgattcgtcattttgaaggcggcgccgcagatggctt
tattatttcaccggcctttctgccgggtagctatgatgaatttgtggatcaggttgttccggtgttacaggatcgtggc
tattttcgcaccgaatatcagggtaataccctgcgtgatcatctgggcttacgcgttccgcagttacagggtcagccga
gctaatactagagactgcaaggacaaccttcatgaccctgtctccggaaaaacagcatgttcgtccgcgtgatgccgca
gataatgatccggttgccgttgcacgtggcctggcagaaaaatggcgcgccaccgccgttgaacgtgatcgtgcaggcg
gttctgccaccgcagaacgtgaagatctgcgcgcctcaggtctgctgtcactgctggttccgcgtgaatatggcggctg
gggtgcagattggccgaccgcaattgaagttgttcgcgaaattgcagccgcagatggctcactgggtcatctgtttggc
tatcatctgaccaatgccccgatgattgaactgattggttcacaggaacaggaagaacatctgtatacccagattgcac
agaacaattggtggaccggtaatgcatcaagcgaaaataactctcatgtgctggattggaaagttcgcgcaaccccgac
cgaagatggcggctatgtgctgaatggtaccaaacatttttgtagcggcgccaaaggtagcgacttgctgtttgtgttt
ggcgttgttcaggatgatagtccgcagcagggcgcaattattgccgcagcaattccgacctctcgcgccggtgtgaccc
cgaatgatgattgggcagccattggcatgcgtcagaccgatagcggctctaccgattttcataatgttaaagtggaacc
ggatgaagtgctgggtgcaccgaatgcctttgtgttagcctttattcagtcagaacgcggtagtctgtttgccccgatt
gcccagttaatttttgccaatgtgtatctaggtattgcacatggtgcattagatgcagcacgcgaatatacccgtaccc
aggcacgtccgtggacccctgccggtattcagcaggccaccgaagatccgtataccattcgcagctacggtgagtttac
cattgcattacagggcgccgatgcagccgcacgcgaagcagcccacctcttacagaccgtgtgggataaaggcgatgcc
ctgaccccggaagatcgtggtgaactgatggttaaagtgagcggcgttaaagccttagcaaccaatgccgccttaaata
tttctagtggtgtgtttgaagttattggtgcacgcggcacccatccgcgctatggctttgatcgcttttggcgtaatgt
tcgtacccatagcttacatgatccggtgagctacaagattgccgatgtgggcaaacataccttaaatggtcagtatccg
attccgggctttacctcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
gcttccggctcgtataatgtgtggaattgtgagcggataacaacagccggagagttcccgatgtctgacaagccgaatg
ccgtttccagccacaccacccccgacgtccccgaagtagcggcgacgcccgagttgtccaccggcatctgcgccggtga
ctaccgcgctgcgcttcgccgccaccccgccggtgtcaccgtcgtgaccctcgattcgggtaccggcccggtgggtttc
accgccacctcgttctcgtccgtctccctcgagccgccgctcgtctcgttcaacatcgcggagacgtcgtcgagcatca
atgcactcaaggcagccgagtccttggtgatccaccttctcggcgaacatcagcagcatctggcccagcgctttgcgcg
tagtgccgatcagcgttttgcagacgagtcactgtgggcagtgctcgacaccggggaaccggtgctgcacggcaccccc
agctggatgcgcgtcaaggtcgaccagctgatccctgtcggcgaccacacgctggtcatcggactcgtcacgcgggttc
acgccgaagaagacgacgaatccgctgccgcgccgctgctctaccacgagggcaagtactaccgcccgactccgttagg
tcaatagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggacaccatcgaatg
gcgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatgtgaaaccagtaacg
ttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctg
cgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcggg
caaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaa
tctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcgg
tgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtgga
agctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccat
gaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaa
gttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacg
ggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatg
ctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatct
cggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcct
gctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtc
tcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgc
agctggcacgacaggtttcccgactggaaagcgggcagtgatactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttata