##gff-version 3
##sequence-region BBa_K1937010 1 6869
BBa_K1937010	.	engineered_region	1	5857	.	+	0	ID=BBa_K1937001;Name=BBa_K1937001
BBa_K1937010	.	primer_binding_site	2546	2565	.	+	0	ID=annotation2497222;Name=Verification forward (VF2) primer binding site ;Parent=BBa_K1937001
BBa_K1937010	.	sequence_feature	3045	4049	.	+	0	ID=annotation2497221;Name=repU (Bacillus subtilis origin);Parent=BBa_K1937001
BBa_K1937010	.	CDS	1564	2223	.	+	0	ID=annotation2497206;Name=Ampicillin resistance (E. coli);Parent=BBa_K1937001
BBa_K1937010	.	sequence_feature	257	278	.	+	0	ID=annotation2497209;Name=Prefix;Parent=BBa_K1937001
BBa_K1937010	.	sequence_feature	784	1466	.	+	0	ID=annotation2497207;Name=ColE1 origin (E. coli);Parent=BBa_K1937001
BBa_K1937010	.	primer_binding_site	685	704	.	+	0	ID=annotation2497223;Name=Verification reverse (VR) primer binding site ;Parent=BBa_K1937001
BBa_K1937010	.	CDS	5202	5600	.	+	0	ID=annotation2497205;Name=Bleomycin resistance (B. subtilis);Parent=BBa_K1937001
BBa_K1937010	.	CDS	4209	4979	.	+	0	ID=annotation2497208;Name=Kanamycin resistance (B. subtilis);Parent=BBa_K1937001
BBa_K1937010	.	sequence_feature	285	305	.	+	0	ID=annotation2497210;Name=Suffixe;Parent=BBa_K1937001
BBa_K1937010	.	engineered_region	5866	6869	.	+	0	ID=BBa_K1937009;Name=BBa_K1937009
BBa_K1937010	.	sequence_feature	6394	6444	.	+	0	ID=annotation2509755;Name=Riboswitch;Parent=BBa_K1937009
BBa_K1937010	.	ribosome_entry_site	6435	6444	.	+	0	ID=annotation2509754;Name=RBS toxin;Parent=BBa_K1937009
BBa_K1937010	.	CDS	6115	6387	.	+	0	ID=annotation2509720;Name=Antitoxin Epsilon;Parent=BBa_K1937009
BBa_K1937010	.	ribosome_entry_site	6103	6114	.	+	0	ID=annotation2509724;Name=RBS Antitoxin;Parent=BBa_K1937009
BBa_K1937010	.	promoter	5866	6102	.	+	0	ID=annotation2509716;Name=Pveg;Parent=BBa_K1937009
BBa_K1937010	.	stop_codon	6790	6869	.	+	0	ID=annotation2509756;Name=Terminator;Parent=BBa_K1937009
BBa_K1937010	.	CDS	6451	6789	.	+	0	ID=annotation2509760;Name=Toxin mazF;Parent=BBa_K1937009
>BBa_K1937010
atctattattaatctgttcagcaatcgggcgcgattgctgaataaaagatacgagagacctctcttgtatcttttttat
tttgagtggttttgtccgttacactagaaaaccgaaagacaataaaaattttattcttgctgagtctggctttcggtaa
gctagacaaaacggacaaaataaaaattggcaagggtttaaaggtggagattttttgagtgatcttctcaaaaaatact
acctgtcccttgctgatttgaattcgcggccgcttctagaggctagctactagtagcggccgctgcaggcgcaacgcaa
ttaatgtgagttaggatcctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacg
gtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggg
gcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatcagagcagattgtactg
agagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggaaaaaataccgcatcaggcgctcttccgcttcct
cgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatc
cacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcg
ttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacc
cgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttac
cggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtg
taggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatc
gtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggta
tgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgct
ctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtt
tttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctga
cgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcctttta
aattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtg
aggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacg
ggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaata
aaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgcc
gggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgccggcatcgtggtgtcacg
ctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaa
aaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcag
cactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctg
agaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaacttta
aaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgt
aacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggca
aaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagc
atttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgca
catttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcac
gaggccctttcgtcttcaagagttccttaaggaacgtacagacggcttaaaagcctttaaaaacgtttttaaggggttt
gtagacaaggtaaaggataaaacagcacaattccaagaaaaacacgatttagaacctaaaaagaacgaatttgaactaa
ctcataaccgagaggtaaaaaaagaacgaagtcgagatcagggaatgagtttataaaataaaaaaagcacctgaaaagg
tgtctttttttgatggttttgaacttgttctttcttatcttgatacatatagaaataacgtcatttttattttagttgc
tgaaaggtgcgttgaagtgttggtatgtatgtgttttaaagtattgaaaacccttaaaattggttgcacagaaaaaccc
catctgttaaagttataagtgactaaacaaataactaaatagatgggggtttcttttaatattatgtgtcctaatagta
gcatttattcagatgaaaaatcaagggttttagtggacaagacaaaaagtggaaaagtgagaccatggagagaaaagaa
aatcgctaatgttgattactttgaacttctgcatattcttgaatttaaaaaggctgaaagagtaaaagattgtgctgaa
atattagagtataaacaaaatcgtgaaacaggcgaaagaaagttgtatcgagtgtggttttgtaaatccaggctttgtc
caatgtgcaactggaggagagcaatgaaacatggcattcagtcacaaaaggttgttgctgaagttattaaacaaaagcc
aacagttcgttggttgtttctcacattaacagttaaaaatgtttatgatggcgaagaattaaataagagtttgtcagat
atggctcaaggatttcgccgaatgatgcaatataaaaaaattaataaaaatcttgttggttttatgcgtgcaacggaag
tgacaataaataataaagataattcttataatcagcacatgcatgtattggtatgtgtggaaccaacttattttaagaa
tacagaaaactacgtgaatcaaaaacaatggattcaattttggaaaaaggcaatgaaattagactatgatccaaatgta
aaagttcaaatgattcgaccgaaaaataaatataaatcggatatacaatcggcaattgacgaaactgcaaaatatcctg
taaaggatacggattttatgaccgatgatgaagaaaagaatttgaaacgtttgtctgatttggaggaaggtttacaccg
taaaaggttaatctcctatggtggtttgttaaaagaaatacataaaaaattaaaccttgatgacacagaagaaggcgat
ttgattcatacagatgatgacgaaaaagccgatgaagatggattttctattattgcaatgtggaattgggaacggaaaa
attattttattaaagagtagttcaacaaacgggccagtttgttgaagattagatgctataattgttattaaaaggattg
aaggatgcttaggaagacgagttattaatagctgaataagaacggtgctctccaaatattcttatttagaaaagcaaat
ctaaaattatctgaaaagggaatgagaatagtgaatggaccaataataatgactagagaagaaagaatgaagattgttc
atgaaattaaggaacgaatattggataaatatggggatgatgttaaggctattggtgtttatggctctcttggtcgtca
gactgatgggccctattcggatattgagatgatgtgtgtcatgtcaacagaggaagcagagttcagccatgaatggaca
accggtgagtggaaggtggaagtgaattttgatagcgaagagattctactagattatgcatctcaggtggaatcagatt
ggccgcttacacatggtcaatttttctctattttgccgatttatgattcaggtggatacttagagaaagtgtatcaaac
tgctaaatcggtagaagcccaaacgttccacgatgcgatttgtgcccttatcgtagaagagctgtttgaatatgcaggc
aaatggcgtaatattcgtgtgcaaggaccgacaacatttctaccatccttgactgtacaggtagcaatggcaggtgcca
tgttgattggtctgcatcatcgcatctgttatacgacgagcgcttcggtcttaactgaagcagttaagcaatcagatct
tccttcaggttatgaccatctgtgccagttcgtaatgtctggtcaactttccgactctgagaaacttctggaatcgcta
gagaatttctggaatgggattcaggagtggacagaacgacacggatatatagtggatgtgtcaaaacgcataccatttt
gaacgatgacctctaataattgttaatcatgttggttacgtatttattaacttctcctagtattagtaattatcatggc
tgtcatggcgcattaacggaataaagggtgtgcttaaatcgggccattttgcgtaataagaaaaaggattaattatgag
cgaattgaattaataataaggtaatagatttacattagaaaatgaaaggggattttatgcgtgagaatgttacagtcta
tcccggcattgccagtcggggatattaaaaagagtataggtttttattgcgataaactaggtttcactttggttcacca
tgaagatggattcgcagttctaatgtgtaatgaggttcggattcatctatgggaggcaagtgatgaaggctggcgctct
cgtagtaatgattcaccggtttgtacaggtgcggagtcgtttattgctggtactgctagttgccgcattgaagtagagg
gaattgatgaattatatcaacatattaagcctttgggcattttgcaccccaatacatcattaaaagatcagtggtggga
tgaacgagactttgcagtaattgatcccgacaacaatttgattagcttttttcaacaaataaaaagctataatctatta
ttaatctgttcagcaatcgggcgcgattgctgaataaaagatacgagagacctctcttgtatcttttttattttgagtg
gttttgtccgttacactagaaaaccgaaagacaataaaaattttattcttgctgagtctggctttcggtaagctagaca
aaacggacaaaataaaaattggcaagggtttaaaggtggagattttttgagtgatcttctcaaaaaatactacctgtcc
cttgctgattttactagagggagttctgagaattggtatgccttataagtccaattaacagttgaaaacctgcatagga
gagctatgcgggttttttattttacataatgatacataatttaccgaaacttgcggaacataattgaggaatcatagaa
ttttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgt
gttgtacaataaatgtagtaaaggtggtgaaatggcagttacgtatgaaaaaacatttgaaatagagatcattaacgaa
ttatcggcaagcgtttataatcgagtattaaactatgttttgaaccatgaattaaataaaaatgactctcaattattgg
aagtcaatttattaaaccaattaaagcttgcaaaacgtgtaaatctttttgattattctttagaagaattacaagccgt
tcatgagtattggcggtcaatgaatcgttactcaaaacaagttttgaataaagagaaagtggcttaaccgcggatgcaa
cctgataccagcatcgtcttgatgcccttggcagcaggcaacaagggtaccatggtaagccgatacgtacccgatatgg
gcgatctgatttgggttgattttgacccgacaaaaggtagcgagcaagctggacatcgtccagctgttgtcctgagtcc
tttcatgtacaacaacaaaacaggtatgtgtctgtgtgttccttgtacaacgcaatcaaaaggatatccgttcgaagtt
gttttatccggtcaggaacgtgatggcgtagcgttagctgatcaggtaaaaagtatcgcctggcgggcaagaggagcaa
cgaagaaaggaacagttgccccagaggaattacaactcattaaagccaaaattaacgtactgattgggtaataaccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc