Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oumnia KARIM
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-17 01:34:08
pNagP-RFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1937005_sequence (Version 1) |
Description
pNagP is the promoter of the NagP gene, which expresses a transporter of N-acetylglucosamine-6-phosphate in Bacillus subtilis.In bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate. This NAG molecule is the major component of chitin, a characteristic molecule of fungi.
This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)
Notes
The BBa_K1937005 part was designed to demonstrate the induction of pNagP by NAG. It belongs to the antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France).The part includes the pNagP promoter and its RBS (position 840,455 to 840,655 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping.
Source
In bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate.| Sequence Annotation | Location | Component / Role(s) |
| Promoter NagP+RBS RFP Terminator | 1,200 207,912 913,992 | promoter feature/promoter feature/protein CDS feature/stop stop_codon |