##gff-version 3
##sequence-region BBa_K193406 1 1743
BBa_K193406	.	engineered_region	1	1743	.	+	0	ID=BBa_K193404;Name=BBa_K193404
BBa_K193406	.	engineered_region	1	836	.	+	0	ID=BBa_K193401;Name=BBa_K193401;Parent=BBa_K193404
BBa_K193406	.	engineered_region	65	836	.	+	0	ID=BBa_K193400;Name=BBa_K193400;Parent=BBa_K193401
BBa_K193406	.	ribosome_entry_site	65	79	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K193400
BBa_K193406	.	ribosome_entry_site	65	79	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K193406	.	ribosome_entry_site	72	76	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K193406	.	engineered_region	65	79	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K193406	.	CDS	86	836	.	+	0	ID=BBa_I15008;Name=BBa_I15008;Parent=BBa_K193400
BBa_K193406	.	engineered_tag	812	836	.	+	0	ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008
BBa_K193406	.	promoter	1	56	.	+	0	ID=BBa_K091112;Name=BBa_K091112;Parent=BBa_K193401
BBa_K193406	.	promoter	12	17	.	+	0	ID=annotation1964077;Name=-35;Parent=BBa_K091112
BBa_K193406	.	promoter	37	41	.	+	0	ID=annotation1964078;Name=-10;Parent=BBa_K091112
BBa_K193406	.	engineered_region	845	1743	.	+	0	ID=BBa_K193403;Name=BBa_K193403;Parent=BBa_K193404
BBa_K193406	.	terminator	1649	1743	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K193403
BBa_K193406	.	terminator	1649	1689	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K193406	.	stem_loop	1656	1675	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K193406	.	stop_codon	1682	1682	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K193406	.	polyA_site	1676	1689	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K193406	.	engineered_region	1649	1689	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K193406	.	terminator	1698	1743	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K193406	.	engineered_region	1698	1743	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K193406	.	stem_loop	1710	1732	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K193406	.	engineered_region	845	1640	.	+	0	ID=BBa_K193402;Name=BBa_K193402;Parent=BBa_K193403
BBa_K193406	.	CDS	866	1640	.	+	0	ID=BBa_I15009;Name=BBa_I15009;Parent=BBa_K193402
BBa_K193406	.	engineered_tag	1616	1640	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_K193406	.	CDS	866	1615	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_K193406	.	ribosome_entry_site	845	859	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K193402
BBa_K193406	.	ribosome_entry_site	845	859	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K193406	.	ribosome_entry_site	852	856	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K193406	.	engineered_region	845	859	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
>BBa_K193406
agcggcatgcatttacgttgacaccacctttcgcggtatggcatgatagcgcccggtactagagattaaagaggagaaa
tactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggct
ttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacag
tgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaa
caaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaag
cctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctggg
ggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctat
gaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaag
ccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaa
cctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctc
gccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagagattaaagaggagaaatactagatgg
ccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgat
cgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaa
ggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgg
gcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgt
ggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtac
caaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctg
aatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaat
tcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattac
tgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtata
tgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttat
tattt