##gff-version 3 ##sequence-region BBa_K1911006 1 4966 BBa_K1911006 . ribosome_entry_site 58 68 . + 0 ID=BBa_B0033;Name=BBa_B0033 BBa_K1911006 . ribosome_entry_site 58 68 . + 0 ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033 BBa_K1911006 . engineered_region 58 68 . + 0 ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033 BBa_K1911006 . ribosome_entry_site 64 67 . + 0 ID=annotation1714;Name=RBS;Parent=BBa_B0033 BBa_K1911006 . tag 1979 2017 . + 0 ID=BBa_M0051;Name=BBa_M0051 BBa_K1911006 . tag 1979 2017 . + 0 ID=annotation1958881;Name=AANDENYNYADAS SsrA deg. tag;Parent=BBa_M0051 BBa_K1911006 . CDS 3543 4166 . + 0 ID=BBa_K1911002;Name=BBa_K1911002 BBa_K1911006 . promoter 2163 2216 . + 0 ID=BBa_R0011;Name=BBa_R0011 BBa_K1911006 . operator 2188 2204 . + 0 ID=annotation2001;Name=lac O1;Parent=BBa_R0011 BBa_K1911006 . engineered_region 2163 2216 . + 0 ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011 BBa_K1911006 . promoter 2182 2187 . + 0 ID=annotation2000;Name=-35;Parent=BBa_R0011 BBa_K1911006 . operator 2165 2181 . + 0 ID=annotation1999;Name=lac O1;Parent=BBa_R0011 BBa_K1911006 . promoter 2205 2210 . + 0 ID=annotation2002;Name=-10;Parent=BBa_R0011 BBa_K1911006 . CDS 4192 4966 . + 0 ID=BBa_C0051;Name=BBa_C0051 BBa_K1911006 . engineered_tag 4942 4966 . + 0 ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051 BBa_K1911006 . CDS 4195 4902 . + 0 ID=annotation23334;Name=cI lambda;Parent=BBa_C0051 BBa_K1911006 . tag 4903 4935 . + 0 ID=annotation23335;Name=LVA;Parent=BBa_C0051 BBa_K1911006 . ribosome_entry_site 3526 3536 . + 0 ID=BBa_B0033;Name=BBa_B0033 BBa_K1911006 . ribosome_entry_site 3526 3536 . + 0 ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033 BBa_K1911006 . engineered_region 3526 3536 . + 0 ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033 BBa_K1911006 . ribosome_entry_site 3532 3535 . + 0 ID=annotation1714;Name=RBS;Parent=BBa_B0033 BBa_K1911006 . CDS 2243 3517 . + 0 ID=BBa_K1911003;Name=BBa_K1911003 BBa_K1911006 . promoter 1 49 . + 0 ID=BBa_R0051;Name=BBa_R0051 BBa_K1911006 . promoter 15 20 . + 0 ID=annotation2023;Name=-35;Parent=BBa_R0051 BBa_K1911006 . engineered_region 1 49 . + 0 ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051 BBa_K1911006 . promoter 38 43 . + 0 ID=annotation2022;Name=-10;Parent=BBa_R0051 BBa_K1911006 . non_covalent_binding_site 25 41 . + 0 ID=annotation2024;Name=OR1;Parent=BBa_R0051 BBa_K1911006 . non_covalent_binding_site 1 17 . + 0 ID=annotation2025;Name=OR2;Parent=BBa_R0051 BBa_K1911006 . CDS 75 1202 . + 0 ID=BBa_C0012;Name=BBa_C0012 BBa_K1911006 . engineered_tag 1203 1227 . + 0 ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012 BBa_K1911006 . CDS 75 1202 . + 0 ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012 BBa_K1911006 . engineered_region 75 1202 . + 0 ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012 BBa_K1911006 . tag 1164 1202 . + 0 ID=annotation1722;Name=LVA;Parent=BBa_C0012 BBa_K1911006 . ribosome_entry_site 1236 1247 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_K1911006 . conserved_region 1240 1243 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K1911006 . CDS 1254 1970 . + 0 ID=BBa_K1911005;Name=BBa_K1911005 BBa_K1911006 . ribosome_entry_site 2226 2236 . + 0 ID=BBa_B0033;Name=BBa_B0033 BBa_K1911006 . ribosome_entry_site 2226 2236 . + 0 ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033 BBa_K1911006 . engineered_region 2226 2236 . + 0 ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033 BBa_K1911006 . ribosome_entry_site 2232 2235 . + 0 ID=annotation1714;Name=RBS;Parent=BBa_B0033 BBa_K1911006 . ribosome_entry_site 4175 4185 . + 0 ID=BBa_B0033;Name=BBa_B0033 BBa_K1911006 . ribosome_entry_site 4175 4185 . + 0 ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033 BBa_K1911006 . engineered_region 4175 4185 . + 0 ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033 BBa_K1911006 . ribosome_entry_site 4181 4184 . + 0 ID=annotation1714;Name=RBS;Parent=BBa_B0033 BBa_K1911006 . terminator 2114 2154 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_K1911006 . stem_loop 2121 2140 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1911006 . stop_codon 2147 2147 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1911006 . polyA_site 2141 2154 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1911006 . engineered_region 2114 2154 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1911006 . terminator 2026 2105 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_K1911006 . stem_loop 2037 2080 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1911006 . engineered_region 2026 2105 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 >BBa_K1911006 taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggactactagatggt gaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaac caggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcg tggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgca aattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtc gaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgacc aggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaa cagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcg ctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaa ttcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgaggg catcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctg cgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccacca tcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaaggg caatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcg ttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacg ctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggctagcaa aggagaagaactcttcactggagttgtcccaattcttgttgaattagatggtgatgttaacggccacaagttctctgtc agtggagagggtgaaggtgatgcaacatacggaaaacttaccctgaagttcatctgcactactggcaaactgcctgttc catggccaaccctggtcactactctgtgctatggtgttcaatgcttttcaagatacccggatcatatgaaacggcatga ctttttcaagagtgccatgcccgaaggttatgtacaggaaaggaccatcttcttcaaagatgacggcaactacaagaca cgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgacttcaaggaagatggca acattctgggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaat caaagtgaacttcaagacccgccacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactcca attggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaa agagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactgtacaattacta gaggctgctaacgacgaaaactacaactacgctgacgcttcttactagagccaggcatcaaataaaacgaaaggctcag tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg gtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactga gcacatactagagtcacacaggactactagatgacagataaacgcaaagatggctcaggcaaattgctgtattgctctt tttgcggcaaaagccagcatgaagtgcgcaagctgattgccggtccatccgtgtatatctgcgacgaatgtgttgattt atgtaacgacatcattcgcgaagagattaaagaagttgcaccgcatcgtgaacgcagtgcgctaccgacgccgcatgaa attcgcaaccacctggacgattacgttatcggccaggaacaggcgaaaaaagtgctggcggtcgcggtatacaaccatt acaaacgtctgcgcaacggcgataccagcaatggcgtcgagttgggcaaaagtaacattctgctgatcggtccgaccgg ttccggtaaaacgctgctggctgaaacgctggcgcgcctgctggatgttccgttcaccatggccgacgcgactacactg accgaagccggttatgtgggtgaagacgttgaaaacatcattcagaagctgttgcagaaatgcgactacgatgtccaga aagcacagcgtggtattgtctacatcgatgaaatcgacaagatttctcgtaagtcagacaacccgtccattacccgaga cgtttccggtgaaggcgtacagcaggcactgttgaaactgatcgaaggtacggtagctgctgttccaccgcaaggtggg cgtaaacatccgcagcaggagttcttgcaggttgatacctctaagatcctgtttatttgtggcggtgcgtttgccggtc tggataaagtgatttcccaccgtgtagaaaccggctccggcattggttttggcgcgacggtaaaagcgaagtccgacaa agcaagcgaaggcgagctgctggcgcaggttgaaccggaagatctgatcaagtttggtcttatccctgagtttattggt cgtctgccggttgtcgcaacgttgaatgaactgagcgaagaagctctgattcagatcctcaaagagccgaaaaacgccc tgaccaagcagtatcaggcgctgtttaatctggaaggcgtggatctggagttccgtgacgaggcgctggatgctatcgc taagaaagcgatggcgcgtaaaaccggtgcccgtggcctgcgttccatcgtagaagccgcactgctcgataccatgtac gatctgccgtccatggaagacgtcgaaaaagtggttatcgacgagtcggtaattgatggtcaaagcaaaccgttgctga tttatggcaagccggaagcgcaacaggcatctggtgaataatactagagtcacacaggactactagatgtcatacagcg gcgaacgagataactttgcaccccatatggcgctggtgccgatggtcattgaacagacctcacgcggtgagcgctcttt tgatatctattctcgtctacttaaggaacgcgtcatttttctgactggccaggttgaagaccacatggctaacctgatt gtggcgcagatgctgttcctggaagcggaaaacccagaaaaagatatctatctgtacattaactccccaggcggggtga tcactgccgggatgtctatctatgacaccatgcagtttatcaagcctgatgtcagcaccatctgtatgggccaggcggc ctcgatgggcgctttcttgctgaccgcaggggcaaaaggtaaacgtttttgcctgccaaattcgcgcgtgatgattcac caaccgttgggcggctaccagggccaggcgaccgatatcgaaattcatgcccgtgaaattctgaaagttaaagggcgca tgaatgaacttatggcgcttcatacgggtcaatcattagaacagattgaacgtgataccgagcgcgatcgcttcctttc cgcccctgaagcggtggaatacggtctggtcgattcgattctgacccatcgtaattgatactagagtcacacaggacta ctagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaa aagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttat ttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttag cccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtac cctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatggg taagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctc caagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagcc agacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccac agtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtt tggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc