##gff-version 3
##sequence-region BBa_K191005 1 1864
BBa_K191005	.	terminator	893	933	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K191005	.	engineered_region	893	933	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K191005	.	stem_loop	900	919	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K191005	.	stop_codon	926	926	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K191005	.	polyA_site	920	933	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K191005	.	terminator	805	884	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K191005	.	engineered_region	805	884	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K191005	.	stem_loop	816	859	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K191005	.	promoter	942	995	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K191005	.	promoter	984	989	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K191005	.	promoter	961	966	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K191005	.	engineered_region	942	995	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K191005	.	non_covalent_binding_site	967	985	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K191005	.	non_covalent_binding_site	942	960	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K191005	.	promoter	1	85	.	+	0	ID=BBa_K191007;Name=BBa_K191007
BBa_K191005	.	ribosome_entry_site	94	105	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K191005	.	conserved_region	98	101	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K191005	.	CDS	112	796	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K191005	.	engineered_tag	772	796	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K191005	.	CDS	115	731	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K191005	.	tag	732	765	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K191005	.	engineered_region	1004	1864	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_K191005	.	CDS	1022	1702	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K191005	.	CDS	1022	1696	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K191005	.	engineered_tag	1703	1727	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K191005	.	terminator	1824	1864	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K191005	.	engineered_region	1824	1864	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K191005	.	stem_loop	1831	1850	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K191005	.	stop_codon	1857	1857	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K191005	.	polyA_site	1851	1864	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K191005	.	ribosome_entry_site	1004	1015	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K191005	.	conserved_region	1008	1011	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K191005	.	terminator	1736	1815	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K191005	.	engineered_region	1736	1815	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K191005	.	stem_loop	1747	1790	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K191005
tggcaaatattctgaaatgagctgttgacaattaatcatcgaactagttaactagtacgcaagttcacgtaaaaagggt
atcgactactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagct
gcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtat
tggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgcc
ctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcga
tggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgc
caacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaag
atcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctat
cgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaa
cttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgta
gatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctat
cagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggct
tcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaa
tcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgcc
gttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggac
tacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgtta
cccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtcc
ggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaa
atcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccgg
ttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaaca
gtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttata