##gff-version 3
##sequence-region BBa_K188009 1 2324
BBa_K188009	.	CDS	68	736	.	+	0	ID=BBa_C0052;Name=BBa_C0052
BBa_K188009	.	CDS	68	736	.	+	0	ID=annotation1743;Name=cI 434;Parent=BBa_C0052
BBa_K188009	.	engineered_region	68	736	.	+	0	ID=annotation7035;Name=BBa_C0052;Parent=BBa_C0052
BBa_K188009	.	engineered_tag	737	761	.	+	0	ID=annotation2213992;Name=Help:Barcodes;Parent=BBa_C0052
BBa_K188009	.	tag	698	736	.	+	0	ID=annotation1745;Name=LVA;Parent=BBa_C0052
BBa_K188009	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K188009	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K188009	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K188009	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K188009	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K188009	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K188009	.	engineered_region	1471	2229	.	+	0	ID=BBa_K145015;Name=BBa_K145015
BBa_K188009	.	CDS	1474	2186	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
BBa_K188009	.	stop_codon	2227	2229	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_K188009	.	start_codon	1471	1473	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
BBa_K188009	.	stop_codon	2224	2226	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_K188009	.	tag	2187	2223	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
BBa_K188009	.	ribosome_entry_site	762	773	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K188009	.	conserved_region	766	769	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K188009	.	terminator	2230	2324	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_K188009	.	terminator	2230	2270	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K188009	.	stem_loop	2237	2256	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K188009	.	engineered_region	2230	2270	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K188009	.	stop_codon	2263	2263	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K188009	.	polyA_site	2257	2270	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K188009	.	terminator	2279	2324	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K188009	.	engineered_region	2279	2324	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K188009	.	stem_loop	2291	2313	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K188009	.	CDS	774	1458	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K188009	.	engineered_tag	1434	1458	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K188009	.	CDS	777	1393	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K188009	.	tag	1394	1427	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K188009	.	ribosome_entry_site	1459	1470	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K188009	.	conserved_region	1463	1466	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K188009	.	ribosome_entry_site	56	67	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K188009	.	conserved_region	60	63	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K188009
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacaaaagaggagaaaatgagtatttct
tccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagt
ctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgt
tgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattg
attagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgaca
gtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcat
ccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgac
gcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggccta
tgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacga
aaactacgctttagtagcttaataactctgatagtgctagtgtagatctcaaagaggagaaaatgtccagattagataa
aagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccag
aagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgaga
tgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaag
ttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaa
actctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtgg
ggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactga
tagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggcctt
gaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttag
tagcttaataacactgatagtgctagtgtagatcacaaagaggagaaaatgcgtaaaggagaagaacttttcactggag
ttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgc
aacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactact
ttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccg
aaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaagg
tgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaa
tacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagac
acaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttt
accagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttctt
gagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacg
ctttagtagcttaataatcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaa
agccagattattaatccggcttttttattattt