##gff-version 3
##sequence-region BBa_K1861010 1 442
BBa_K1861010	.	CDS	266	313	.	+	0	ID=BBa_K1861103;Name=BBa_K1861103
BBa_K1861010	.	stop_codon	311	313	.	+	0	ID=annotation2476102;Name=TGA stop codon;Parent=BBa_K1861103
BBa_K1861010	.	sequence_feature	305	310	.	+	0	ID=annotation2476103;Name=SacI restriction site;Parent=BBa_K1861103
BBa_K1861010	.	promoter	1	238	.	+	0	ID=BBa_K1861101;Name=BBa_K1861101
BBa_K1861010	.	sequence_alteration	133	133	.	+	0	ID=annotation2476062;Name=T->C mutation to avoid SpeI restriction site;Parent=BBa_K1861101
BBa_K1861010	.	promoter	1	238	.	+	0	ID=annotation2476069;Name=rpd3 promoter;Parent=BBa_K1861101
BBa_K1861010	.	terminator	314	442	.	+	0	ID=BBa_K392003;Name=BBa_K392003
BBa_K1861010	.	tag	239	265	.	+	0	ID=BBa_K1861102;Name=BBa_K1861102
BBa_K1861010	.	sequence_feature	260	265	.	+	0	ID=annotation2476068;Name=BamHI restriction site;Parent=BBa_K1861102
BBa_K1861010	.	tag	242	259	.	+	0	ID=annotation2476067;Name=6x His-Tag;Parent=BBa_K1861102
BBa_K1861010	.	start_codon	239	241	.	+	0	ID=annotation2476066;Name=ATG start codon;Parent=BBa_K1861102
>BBa_K1861010
gtaatatcaactcagagcgtataggtaaatttgtaaatatatgtcccatattttgccttgaaatttatctttttattta
cttaccctccccggctcatagtatctatcgaaagggaaaaacagaaaagataccagtagttgattatacataataaaaa
tttgaagtaataaccataaaggttcataaaacaattgcgccatacaaaacattcgtggctacaactcgatatccgtgca
gatgcatcaccaccatcatcacggatccacgcacatttctataagtgcttcaccggcctgcgaacgcgagctctgagca
tgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcag
cataaattactatacttctattactagagcccgccgccaccatggag