##gff-version 3
##sequence-region BBa_K185004 1 443
BBa_K185004	.	terminator	403	443	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K185004	.	polyA_site	430	443	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K185004	.	engineered_region	403	443	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K185004	.	stem_loop	410	429	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K185004	.	stop_codon	436	436	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K185004	.	terminator	315	394	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K185004	.	stem_loop	326	369	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K185004	.	engineered_region	315	394	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K185004	.	CDS	1	306	.	+	0	ID=BBa_K185047;Name=BBa_K185047
BBa_K185004	.	stop_codon	306	306	.	+	0	ID=annotation2046574;Name=stop;Parent=BBa_K185047
BBa_K185004	.	CDS	4	285	.	+	0	ID=annotation2046560;Name=cds;Parent=BBa_K185047
BBa_K185004	.	start_codon	1	3	.	+	0	ID=annotation2046533;Name=start;Parent=BBa_K185047
BBa_K185004	.	tag	286	303	.	+	0	ID=annotation2047133;Name=His tag;Parent=BBa_K185047
BBa_K185004	.	sequence_alteration	305	306	.	+	0	ID=annotation2047034;Name=mutation;Parent=BBa_K185047
>BBa_K185004
atggcgtattttctggattttgacgagcgggcactaaaggaatggcgaaagctgggctcgacggtacgtgaacagttga
aaaagaagctggttgaagtacttgagtcaccccggattgaagcaaacaagctccgtggtatgcctgattgttacaagat
taagctccggtcttcaggctatcgccttgtataccaggttatagacgagaaagttgtcgttttcgtgatttctgttggg
aaaagagaacgctcggaagtatatagcgaggcggtcaaacgcattctccatcatcatcatcatcactaatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttata