##gff-version 3
##sequence-region BBa_K182003 1 2071
BBa_K182003	.	engineered_region	1	760	.	+	0	ID=BBa_K182002;Name=BBa_K182002
BBa_K182003	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_K182002
BBa_K182003	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K182003	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K182003	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K182003	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K182003	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K182003	.	engineered_region	58	760	.	+	0	ID=BBa_S03518;Name=BBa_S03518;Parent=BBa_K182002
BBa_K182003	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03518
BBa_K182003	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K182003	.	CDS	76	760	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S03518
BBa_K182003	.	engineered_tag	736	760	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K182003	.	tag	696	729	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K182003	.	CDS	79	695	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K182003	.	engineered_region	769	2071	.	+	0	ID=BBa_K182001;Name=BBa_K182001
BBa_K182003	.	terminator	1881	1960	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K182001
BBa_K182003	.	stem_loop	1892	1935	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K182003	.	engineered_region	1881	1960	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K182003	.	promoter	2018	2071	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K182001
BBa_K182003	.	promoter	2037	2042	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K182003	.	non_covalent_binding_site	2043	2061	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K182003	.	promoter	2060	2065	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K182003	.	non_covalent_binding_site	2018	2036	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K182003	.	engineered_region	2018	2071	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K182003	.	terminator	1969	2009	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K182001
BBa_K182003	.	stop_codon	2002	2002	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K182003	.	stem_loop	1976	1995	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K182003	.	polyA_site	1996	2009	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K182003	.	engineered_region	1969	2009	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K182003	.	ribosome_entry_site	769	780	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K182001
BBa_K182003	.	conserved_region	773	776	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K182003	.	CDS	787	1872	.	+	0	ID=BBa_I732100;Name=BBa_I732100;Parent=BBa_K182001
>BBa_K182003
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgt
ccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccg
taaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgcc
ttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgta
ataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacaga
aaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgca
ctcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaa
cacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcctt
cttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaa
aactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatga
aaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccag
ccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaa
caactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcg
cggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctg
taaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgcc
attgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtatta
ttttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagc
gggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccg
atagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttc
ccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttgg
tgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacag
gattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagc
tgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccga
ttcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataatactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtg
atagagatactgagcac