##gff-version 3
##sequence-region BBa_K182001 1 1303
BBa_K182001	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K182001	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K182001	.	terminator	1201	1241	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K182001	.	polyA_site	1228	1241	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K182001	.	stem_loop	1208	1227	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K182001	.	engineered_region	1201	1241	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K182001	.	stop_codon	1234	1234	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K182001	.	terminator	1113	1192	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K182001	.	stem_loop	1124	1167	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K182001	.	engineered_region	1113	1192	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K182001	.	CDS	19	1104	.	+	0	ID=BBa_I732100;Name=BBa_I732100
BBa_K182001	.	promoter	1250	1303	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K182001	.	promoter	1269	1274	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K182001	.	promoter	1292	1297	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K182001	.	non_covalent_binding_site	1250	1268	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K182001	.	engineered_region	1250	1303	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K182001	.	non_covalent_binding_site	1275	1293	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
>BBa_K182001
aaagaggagaaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgttt
cccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaatta
cattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctg
cacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtag
aacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaacta
tccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgac
cagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtc
accagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatct
cactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaa
atgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccatta
ccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatccc
gccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggc
caggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccg
cctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgata
gagattgacatccctatcagtgatagagatactgagcac