##gff-version 3
##sequence-region BBa_K1813065 1 5456
BBa_K1813065	.	engineered_region	3635	5456	.	+	0	ID=BBa_K1813052;Name=BBa_K1813052
BBa_K1813065	.	terminator	5362	5456	.	+	0	ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1813052
BBa_K1813065	.	terminator	5362	5456	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017
BBa_K1813065	.	terminator	5362	5402	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1813065	.	stop_codon	5395	5395	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1813065	.	polyA_site	5389	5402	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1813065	.	stem_loop	5369	5388	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1813065	.	engineered_region	5362	5402	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1813065	.	terminator	5411	5456	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1813065	.	engineered_region	5411	5456	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1813065	.	stem_loop	5423	5445	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1813065	.	CDS	3722	5353	.	+	0	ID=BBa_K1813051;Name=BBa_K1813051;Parent=BBa_K1813052
BBa_K1813065	.	sequence_feature	3722	3787	.	+	0	ID=annotation2449887;Name=pelB;Parent=BBa_K1813051
BBa_K1813065	.	engineered_region	3635	3715	.	+	0	ID=BBa_K1813037;Name=BBa_K1813037;Parent=BBa_K1813052
BBa_K1813065	.	promoter	3635	3695	.	+	0	ID=BBa_K864400;Name=BBa_K864400;Parent=BBa_K1813037
BBa_K1813065	.	operator	3670	3695	.	+	0	ID=annotation2197240;Name=lacO1 site;Parent=BBa_K864400
BBa_K1813065	.	promoter	3663	3667	.	+	0	ID=annotation2197238;Name=-10;Parent=BBa_K864400
BBa_K1813065	.	promoter	3641	3646	.	+	0	ID=annotation2197237;Name=-35;Parent=BBa_K864400
BBa_K1813065	.	promoter	3635	3674	.	+	0	ID=annotation2197236;Name=Ptac;Parent=BBa_K864400
BBa_K1813065	.	ribosome_entry_site	3704	3715	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1813037
BBa_K1813065	.	conserved_region	3708	3711	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1813065	.	engineered_region	1	3626	.	+	0	ID=BBa_K1813059;Name=BBa_K1813059
BBa_K1813065	.	engineered_region	1328	3626	.	+	0	ID=BBa_K1813054;Name=BBa_K1813054;Parent=BBa_K1813059
BBa_K1813065	.	terminator	3532	3626	.	+	0	ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1813054
BBa_K1813065	.	terminator	3532	3626	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017
BBa_K1813065	.	terminator	3532	3572	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1813065	.	stop_codon	3565	3565	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1813065	.	polyA_site	3559	3572	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1813065	.	stem_loop	3539	3558	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1813065	.	engineered_region	3532	3572	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1813065	.	terminator	3581	3626	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1813065	.	engineered_region	3581	3626	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1813065	.	stem_loop	3593	3615	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1813065	.	engineered_region	1328	1408	.	+	0	ID=BBa_K1813037;Name=BBa_K1813037;Parent=BBa_K1813054
BBa_K1813065	.	promoter	1328	1388	.	+	0	ID=BBa_K864400;Name=BBa_K864400;Parent=BBa_K1813037
BBa_K1813065	.	operator	1363	1388	.	+	0	ID=annotation2197240;Name=lacO1 site;Parent=BBa_K864400
BBa_K1813065	.	promoter	1356	1360	.	+	0	ID=annotation2197238;Name=-10;Parent=BBa_K864400
BBa_K1813065	.	promoter	1334	1339	.	+	0	ID=annotation2197237;Name=-35;Parent=BBa_K864400
BBa_K1813065	.	promoter	1328	1367	.	+	0	ID=annotation2197236;Name=Ptac;Parent=BBa_K864400
BBa_K1813065	.	ribosome_entry_site	1397	1408	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1813037
BBa_K1813065	.	conserved_region	1401	1404	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1813065	.	CDS	1415	3523	.	+	0	ID=BBa_K1813053;Name=BBa_K1813053;Parent=BBa_K1813054
BBa_K1813065	.	sequence_feature	1415	1480	.	+	0	ID=annotation2449907;Name=pelB;Parent=BBa_K1813053
BBa_K1813065	.	promoter	1	1319	.	+	0	ID=BBa_K1813019;Name=BBa_K1813019;Parent=BBa_K1813059
>BBa_K1813065
aaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccga
aggtgagccagtgtgactctagtagagatctacactagcactatcagagttattaagctactaaagcgtagttttcgtc
gtttgcagcctgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagag
gcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcct
ggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacgg
cgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcg
gtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagca
tttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagt
gagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctgg
tgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtct
ggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcgg
atagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgt
tctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgt
gcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaat
gtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgg
gaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccatctagtatt
tctcctctttctctagtaggcgctatcatgccataccgcgaaaggttttgcaccatactagaggagctgttgacaatta
atcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgaaata
cctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatggacgctcagaccgaaacc
gaagtgccagcaggctctgtgagtgatgaacctttcttaggtcctttagacatcgttttattggtatctttattggccg
gtacagcatggtatttgttgaaaggcaagaaaaaagaaagtcaggcatcacagtttaaatcatatagtattcaaccaac
aaccgtgaatacaatgaccatggtggaaaacagtttcatcaagaaattacagagttctggtcgtcgcttagttgttttt
tatggttcacagaccggtacagcagaagaatttgccggtcgcttagctaaagaaggcatccgttatcagatgaaaggta
tggtagcagatccagaagaatgtaacatggaagagttgttaatgttaaaagacatcgacaagtcattggctgtgttttg
cttagcaacctatggtgaaggtgatccaaccgataactgtatggaattttatgattggatccagaacaatgatttagat
atgactggcttgaattacgctgtgtttggtttgggtaataaaacctacgagcattataataaagttggcatttacgttg
ataaacgcttggaagaattgggtgcaaaccgtgtttttgaattgggcttgggcgatgacgatgccaacattgaagatta
tttcatcacctggaaagaaaagttttggccaacagtttgcgattatttcggtattgaaagtacaggcgaagacgttttg
atgcgtcagtatcgcttattggagcaaccagatgtgtcagccgatcgcatttacaccggcgaggtggcacgcttacaca
gtttgcaaacccaacgcccaccttttgacgccaaaaaccctttcttggctccaatcaaagttaatcgtgagttgcataa
agcaggtggccgtagttgcatgcatgtggaatttgatatcgaaggtagtaaaatgcgttatgaggctggtgaccattta
gccatgtatcctgttaacgatcgcgatttggttgagcgtttaggtcgtttatgtaacgctgaattggacaccgttttct
ctttaatcaacacagacacagattcttcaaaaaaacatccattcccttgcccaaccacttaccgtactgccttaaccca
ctacttagaaatcacagccttgccacgtacccacattttgaaagaattagctgaatattgcggtgaggaaaaagataaa
gaatttttgcgttttatctcttctaccgcaccagatggcaaggcaaagtatcaggagtgggttcaggatagttgtcgca
acatcgtacacgtgttggaagacattccttcttgccaccctcctattgaccacgtgtgtgagttattaccacgtttaca
acctcgctaccactctatttcttctagttcaaaattacaccctaccacagttcatgtgaccgcagtattagtaaaatat
gaaaccaaaaccggtcgcttaaataaaggtgtagctaccactttcttggccgagaaacatccaaatgatggcgaaccag
ccccacgtgttccaatttttatccgcaaatctcagtttcgtttgcctccaaaaccagaaaccccagtgattatggtggg
cccaggtaccggtttggctcctttccgtggttttattcaggagcgcgaccattgcaaacaggagggcaaagaaattggt
cagaccactttatattttggctgtcgcaaacgtagtgaagactatatctatgaagacgaattggaggactacagtaaac
gcggcatcatcaatttacgtgtggccttttcacgcgatcaagagaaaaaagtgtatgtgactcacttattagaacagga
ctctgatttgatttggagtgtgatcggtgaaaataagggtcatttctatatttgcggtgacgccaaaaacatggctact
gatgtgcgcaacatcttgttgaaagtaattcgtagtaaaggcggcttatcagagaccgaagctcaacaatacatcaaga
agatggaggcacagaagcgttattctgctgatgtatggtcataataatactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagag
gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggaga
aatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatgga
attattagaaatcgtaaagtcagccatggatacacattctgtattattattcttatcagtgatggtatatttgttatac
gtgtatcgcgataagtttcattactggtcaaaacgtggcgttccttgccaaagtccagcacagtcaattgttcgtacct
tccgtttagtattgcgcatggattcttttaccgacaacttctatcgtgtgtataaggcattcgacggtcatccttatgt
gggttctttagagttaaccaaaccaatcttagtagtgcgcgatcctgaattggcacgcattgtgttagttaaatctttt
agtagtttttctggccgtttaaaatctccagacacaacattggacccattaagtaatcatttattcactttaaacggtg
aaaaatggcgccaggtacgtcacaaaactgccaccgctttttctaccgccaagttaaaaaacatgtttcacagtttgaa
ggattgtgctcgtgaaatggatgcctatatggaacgcgcaattggtgataaaggcgatgtggaatttgatgcttttaaa
gtgatgtctaattatactttagaagttatcggcgcttgtgctatgggcatcaaatgcgattctatccatgacgaagaaa
ccgaatttaaacgtttaagtcgcgatttctttcgttttgacgctcgccgcatgatcttcacattgttggatttattaca
ccctaaattgcctgttttattgaaatggaaagcagtacgccctgaggtagaaaacttcttccgcgaagccattaaagaa
gcagcttctttgaaagagtcagaagcagccgctcgcaccgattttttacagattttaatcgattttcagaaatctgaaa
aagcttcaaaaaccgatgctggtaacgacactgaattggtgtttaccgataacatcattggtggcgttatcggttcttt
cttttcagctggctatgagcctaccgctgctgctttgactttttgtttgtatgaattagctcgtcaccctcaggttcag
gccaaattacatgaaggcatcttagcagtaaaggaaaagttgggcgatgatatcgaatatgaaaacttaaaagagttta
aatatgcaaatcaagtaatcgatgaaactttgcgtttgtaccctgccagtggtattttggtgcgtacttgtacagagcc
ttttaaattaccagattctgacgttgtgattgaaaaaggcacaaaagtgttcgtgtcatcatatggcttacagaccgat
cctcgctactttcctgagcctgagaaatttgatccagaacgcttctcagaagaaaataaagagaaaattttgccaggta
cctacttaactttcggtgatggccctcgcttatgcattgcaatgcgtttagctttgatggacgtgaaaatgatgatggt
tcgtttggtgtcaaaatatgaaattcataccaccccaaagaccccaaaaaaaattacctttgacactaactcatttact
gtgcagccagccgaaaaagtgtggttacgttttcgccgccgcgcaagtaccccttaataatactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttat
tattt