##gff-version 3
##sequence-region BBa_K1769002 1 5050
BBa_K1769002	.	engineered_region	4173	5050	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K1769002	.	terminator	5010	5050	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K1769002	.	polyA_site	5037	5050	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1769002	.	engineered_region	5010	5050	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1769002	.	stop_codon	5043	5043	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1769002	.	stem_loop	5017	5036	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1769002	.	ribosome_entry_site	4173	4187	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K1769002	.	engineered_region	4173	4187	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1769002	.	ribosome_entry_site	4180	4184	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1769002	.	ribosome_entry_site	4173	4187	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1769002	.	CDS	4194	4913	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K1769002	.	CDS	4194	4913	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1769002	.	terminator	4922	5001	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K1769002	.	engineered_region	4922	5001	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1769002	.	stem_loop	4933	4976	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1769002	.	CDS	3151	3939	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_K1769002	.	engineered_region	3151	3939	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K1769002	.	tag	3901	3933	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K1769002	.	CDS	3151	3900	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K1769002	.	stop_codon	3934	3939	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K1769002	.	start_codon	3151	3153	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K1769002	.	engineered_tag	3940	3964	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K1769002	.	promoter	4110	4164	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K1769002	.	non_covalent_binding_site	4110	4129	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1769002	.	promoter	4151	4156	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1769002	.	promoter	4129	4134	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1769002	.	start_codon	4162	4162	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1769002	.	engineered_region	4110	4164	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1769002	.	terminator	3973	4101	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1769002	.	terminator	3973	4052	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1769002	.	engineered_region	3973	4052	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1769002	.	stem_loop	3984	4027	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1769002	.	terminator	4061	4101	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1769002	.	polyA_site	4088	4101	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1769002	.	engineered_region	4061	4101	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1769002	.	stop_codon	4094	4094	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1769002	.	stem_loop	4068	4087	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1769002	.	terminator	2953	3081	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1769002	.	terminator	2953	3032	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1769002	.	engineered_region	2953	3032	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1769002	.	stem_loop	2964	3007	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1769002	.	terminator	3041	3081	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1769002	.	polyA_site	3068	3081	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1769002	.	engineered_region	3041	3081	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1769002	.	stop_codon	3074	3074	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1769002	.	stem_loop	3048	3067	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1769002	.	ribosome_entry_site	1277	1288	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1769002	.	conserved_region	1281	1284	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1769002	.	promoter	3090	3144	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K1769002	.	non_covalent_binding_site	3090	3109	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1769002	.	promoter	3131	3136	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1769002	.	promoter	3109	3114	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1769002	.	start_codon	3142	3142	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1769002	.	engineered_region	3090	3144	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1769002	.	CDS	1295	2050	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K1769002	.	engineered_region	1295	1296	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1769002	.	engineered_region	1295	2050	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1769002	.	engineered_tag	2051	2075	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1769002	.	point_mutation	1468	1468	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1769002	.	point_mutation	1786	1786	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1769002	.	CDS	1295	2044	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1769002	.	promoter	1	1268	.	+	0	ID=BBa_J61051;Name=BBa_J61051
BBa_K1769002	.	CDS	2302	2919	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K1769002	.	CDS	2302	2880	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1769002	.	engineered_region	2302	2919	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1769002	.	tag	2881	2912	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1769002	.	engineered_tag	2920	2944	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1769002	.	promoter	2221	2275	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K1769002	.	non_covalent_binding_site	2221	2240	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1769002	.	promoter	2262	2267	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1769002	.	promoter	2240	2245	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1769002	.	start_codon	2273	2273	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1769002	.	engineered_region	2221	2275	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1769002	.	terminator	2084	2212	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1769002	.	terminator	2084	2163	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1769002	.	engineered_region	2084	2163	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1769002	.	stem_loop	2095	2138	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1769002	.	terminator	2172	2212	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1769002	.	polyA_site	2199	2212	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1769002	.	engineered_region	2172	2212	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1769002	.	stop_codon	2205	2205	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1769002	.	stem_loop	2179	2198	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1769002	.	ribosome_entry_site	2284	2295	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1769002	.	conserved_region	2288	2291	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1769002
ggccgctgcgatcccgcgaagaaccaaaaaagctcgacagagggcgcggtcattttaggtcgggcggatcggcgccgcc
ggctcggctggtgtgccgcacagcaccgcctacgtgagctgccagttgatgaacttcccccgttgccagctagggcgca
agcgggctgtataagatcactgcccatcacattgatcggctcggattttttctcaatccgtaaacaggtcaaacatcag
ttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacg
actgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagat
cggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgt
catgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccaga
gtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaaga
agccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcga
actgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatc
tcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgt
tgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgt
gcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccagg
ttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgca
gttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcga
gtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatagag
gatctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaatta
aagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttact
cgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatat
tatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaata
tatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtt
tagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagat
agtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatag
caaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttg
ggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaataca
acaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacact
gatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
tactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagagga
gaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagt
cttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgata
actcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacagg
tgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaatta
agtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagcta
tatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcg
tattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatg
cctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactct
gatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
tactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagatgacagtaa
agaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaatt
attagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagt
gcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaa
tcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatca
tgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaa
gaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttc
aattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattatt
aacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagcttta
tcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaa
ggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtg
ctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactaga
gacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaa
tactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggc
acaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactgg
aaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcat
atgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacg
ggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattt
taaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaa
caaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatc
aacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaa
agatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaa
ctatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata