##gff-version 3
##sequence-region BBa_K176166 1 670
BBa_K176166	.	CDS	21	533	.	+	0	ID=BBa_K176158;Name=BBa_K176158
BBa_K176166	.	point_mutation	185	185	.	+	0	ID=annotation2020923;Name=g and 69bp in I732006;Parent=BBa_K176158
BBa_K176166	.	primer_binding_site	61	84	.	+	0	ID=annotation2020921;Name=M13 -47 primer (K176059);Parent=BBa_K176158
BBa_K176166	.	engineered_region	192	494	.	+	0	ID=annotation2020925;Name=ccdB (K145151);Parent=BBa_K176158
BBa_K176166	.	primer_binding_site	41	57	.	+	0	ID=annotation2020920;Name=M13 -20 primer;Parent=BBa_K176158
BBa_K176166	.	point_mutation	275	275	.	+	0	ID=annotation2020927;Name=c in K145151;Parent=BBa_K176158
BBa_K176166	.	sequence_alteration	36	36	.	+	0	ID=annotation2020919;Name=177bp and a in J32026;Parent=BBa_K176158
BBa_K176166	.	primer_binding_site	61	77	.	+	0	ID=annotation2020922;Name=M13 -40 primer;Parent=BBa_K176158
BBa_K176166	.	tag	495	527	.	+	0	ID=annotation2020914;Name=LVA degradation tag (AANDENYALVA);Parent=BBa_K176158
BBa_K176166	.	engineered_region	21	185	.	+	0	ID=annotation2020917;Name=a part of lacZalpha (I732006);Parent=BBa_K176158
BBa_K176166	.	stop_codon	528	533	.	+	0	ID=annotation2020916;Name=Stop;Parent=BBa_K176158
BBa_K176166	.	primer_binding_site	208	235	.	+	0	ID=annotation2020926;Name=VRccdB primer (K176058);Parent=BBa_K176158
BBa_K176166	.	sequence_alteration	192	193	.	+	0	ID=annotation2020924;Name=at in K145151;Parent=BBa_K176158
BBa_K176166	.	CDS	21	494	.	+	0	ID=annotation2020913;Name=lacZalpha-ccdB;Parent=BBa_K176158
BBa_K176166	.	start_codon	21	23	.	+	0	ID=annotation2020918;Name=Start;Parent=BBa_K176158
BBa_K176166	.	ribosome_entry_site	1	14	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_K176166	.	conserved_region	7	10	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K176166	.	terminator	630	670	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K176166	.	stem_loop	637	656	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176166	.	polyA_site	657	670	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176166	.	stop_codon	663	663	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176166	.	engineered_region	630	670	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176166	.	terminator	542	621	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K176166	.	stem_loop	553	596	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176166	.	engineered_region	542	621	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K176166
tcacacaggaaacctactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccc
tggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgat
cgcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtc
tgtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgct
gtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatg
gccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccatta
acctgatgttctggggaatagctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata