##gff-version 3
##sequence-region BBa_K176124 1 1899
BBa_K176124	.	engineered_region	1	936	.	+	0	ID=BBa_I0462;Name=BBa_I0462
BBa_K176124	.	CDS	19	774	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176124	.	engineered_region	19	774	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176124	.	point_mutation	510	510	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176124	.	engineered_tag	775	799	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176124	.	point_mutation	192	192	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176124	.	engineered_region	19	20	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176124	.	CDS	19	768	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176124	.	terminator	896	936	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176124	.	polyA_site	923	936	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176124	.	stop_codon	929	929	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176124	.	engineered_region	896	936	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176124	.	stem_loop	903	922	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176124	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176124	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176124	.	terminator	808	887	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176124	.	stem_loop	819	862	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176124	.	engineered_region	808	887	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176124	.	engineered_region	945	1899	.	+	0	ID=BBa_K176002;Name=BBa_K176002
BBa_K176124	.	promoter	945	1016	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176002
BBa_K176124	.	non_covalent_binding_site	998	1016	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176124	.	promoter	986	991	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176124	.	promoter	964	969	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176124	.	non_covalent_binding_site	945	964	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176124	.	start_codon	997	997	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176124	.	engineered_region	945	997	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176124	.	terminator	1771	1850	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176002
BBa_K176124	.	stem_loop	1782	1825	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176124	.	engineered_region	1771	1850	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176124	.	terminator	1859	1899	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176002
BBa_K176124	.	polyA_site	1886	1899	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176124	.	stop_codon	1892	1892	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176124	.	engineered_region	1859	1899	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176124	.	stem_loop	1866	1885	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176124	.	CDS	1043	1762	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K176002
BBa_K176124	.	CDS	1043	1762	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K176124	.	ribosome_entry_site	1025	1036	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K176002
BBa_K176124	.	conserved_region	1029	1032	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K176124
aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaa
gcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattta
tcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgct
aatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaaca
atgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctat
tcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttta
catgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaat
caaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaa
aatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgc
caaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagt
gtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacct
gtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagaaa
gaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatg
ttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttg
cactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatac
ccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttca
aagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaagg
tattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatg
gcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcag
accattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgc
cctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggc
atggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
ata