##gff-version 3
##sequence-region BBa_K176029 1 2732
BBa_K176029	.	engineered_region	791	2732	.	+	0	ID=BBa_K176026;Name=BBa_K176026
BBa_K176029	.	engineered_region	791	1769	.	+	0	ID=BBa_K176025;Name=BBa_K176025;Parent=BBa_K176026
BBa_K176029	.	promoter	791	825	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176025
BBa_K176029	.	engineered_region	834	1769	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176025
BBa_K176029	.	terminator	1729	1769	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176029	.	stop_codon	1762	1762	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176029	.	polyA_site	1756	1769	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176029	.	stem_loop	1736	1755	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176029	.	engineered_region	1729	1769	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176029	.	CDS	852	1607	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176029	.	engineered_tag	1608	1632	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176029	.	engineered_region	852	1607	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176029	.	engineered_region	852	853	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176029	.	point_mutation	1025	1025	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176029	.	CDS	852	1601	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176029	.	point_mutation	1343	1343	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176029	.	terminator	1641	1720	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176029	.	engineered_region	1641	1720	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176029	.	stem_loop	1652	1695	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176029	.	ribosome_entry_site	834	845	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176029	.	conserved_region	838	841	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176029	.	engineered_region	1778	2732	.	+	0	ID=BBa_K176002;Name=BBa_K176002;Parent=BBa_K176026
BBa_K176029	.	terminator	2604	2683	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176002
BBa_K176029	.	engineered_region	2604	2683	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176029	.	stem_loop	2615	2658	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176029	.	ribosome_entry_site	1858	1869	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K176002
BBa_K176029	.	conserved_region	1862	1865	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176029	.	CDS	1876	2595	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K176002
BBa_K176029	.	CDS	1876	2595	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K176029	.	promoter	1778	1849	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176002
BBa_K176029	.	promoter	1797	1802	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176029	.	non_covalent_binding_site	1778	1797	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176029	.	promoter	1819	1824	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176029	.	non_covalent_binding_site	1831	1849	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176029	.	start_codon	1830	1830	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176029	.	engineered_region	1778	1830	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176029	.	terminator	2692	2732	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176002
BBa_K176029	.	stop_codon	2725	2725	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176029	.	polyA_site	2719	2732	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176029	.	stem_loop	2699	2718	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176029	.	engineered_region	2692	2732	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176029	.	engineered_region	1	782	.	+	0	ID=BBa_K176001;Name=BBa_K176001
BBa_K176029	.	CDS	19	645	.	+	0	ID=BBa_I732080;Name=BBa_I732080;Parent=BBa_K176001
BBa_K176029	.	CDS	19	645	.	+	0	ID=annotation1940756;Name=tetR;Parent=BBa_I732080
BBa_K176029	.	terminator	742	782	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176001
BBa_K176029	.	stop_codon	775	775	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176029	.	polyA_site	769	782	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176029	.	stem_loop	749	768	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176029	.	engineered_region	742	782	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176029	.	terminator	654	733	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176001
BBa_K176029	.	engineered_region	654	733	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176029	.	stem_loop	665	708	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176029	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K176001
BBa_K176029	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K176029
aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcg
gaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaa
taagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaa
agctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtac
atttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttc
actagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaa
gtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatc
accaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaag
tgggtcctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgcc
gacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatga
ctaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcct
agataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattct
aactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaag
aagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttt
tgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttct
ctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtt
tagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcca
tttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaatt
gattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttg
ttatagtcgaatatccctatcagtgatagagatactagagaaagaggagaaatactagatgcgtaaaggagaagaactt
ttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtg
aaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacact
tgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagt
gccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtca
agtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggaca
caaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttc
aaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggcc
ctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacat
ggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttata