##gff-version 3
##sequence-region BBa_K176003 1 480
BBa_K176003	.	point_mutation	165	165	.	+	0	ID=annotation2008006;Name=g and 69bp in I732006
BBa_K176003	.	engineered_region	1	165	.	+	0	ID=annotation2008000;Name=a part of lacZalpha (I732006)
BBa_K176003	.	primer_binding_site	21	37	.	+	0	ID=annotation2008003;Name=M13 -20 primer
BBa_K176003	.	sequence_alteration	16	16	.	+	0	ID=annotation2008002;Name=177bp and a in J32026
BBa_K176003	.	primer_binding_site	188	215	.	+	0	ID=annotation2011971;Name=VRccdB primer (K176058)
BBa_K176003	.	CDS	1	474	.	+	0	ID=annotation2020665;Name=lacZalpha-ccdB
BBa_K176003	.	stop_codon	475	480	.	+	0	ID=annotation2008010;Name=Stop
BBa_K176003	.	point_mutation	255	255	.	+	0	ID=annotation2008009;Name=c in K145151
BBa_K176003	.	start_codon	1	3	.	+	0	ID=annotation2008001;Name=Start
BBa_K176003	.	engineered_region	172	477	.	+	0	ID=annotation2008008;Name=ccdB (K145151)
BBa_K176003	.	sequence_alteration	172	173	.	+	0	ID=annotation2008007;Name=at in K145151
BBa_K176003	.	primer_binding_site	41	64	.	+	0	ID=annotation2008004;Name=M13 -47 primer (K176059)
BBa_K176003	.	primer_binding_site	41	57	.	+	0	ID=annotation2008005;Name=M13 -40 primer
>BBa_K176003
atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatc
gccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcg
cagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagt
gatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtg
aactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgt
tatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaata
taataa