##gff-version 3 ##sequence-region BBa_K175045 1 3028 BBa_K175045 . engineered_region 1123 3028 . + 0 ID=BBa_K175044;Name=BBa_K175044 BBa_K175045 . sequence_feature 1123 1152 . + 0 ID=BBa_K175027;Name=BBa_K175027;Parent=BBa_K175044 BBa_K175045 . sequence_feature 1123 1152 . + 0 ID=annotation2014585;Name=I-SceI homing endonuclease restriction site;Parent=BBa_K175027 BBa_K175045 . engineered_region 1161 3028 . + 0 ID=BBa_K145280;Name=BBa_K145280;Parent=BBa_K175044 BBa_K175045 . engineered_region 1161 2043 . + 0 ID=BBa_K145201;Name=BBa_K145201;Parent=BBa_K145280 BBa_K175045 . ribosome_entry_site 1204 1215 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K145201 BBa_K175045 . conserved_region 1208 1211 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K175045 . CDS 1222 1906 . + 0 ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K145201 BBa_K175045 . tag 1842 1875 . + 0 ID=annotation23330;Name=SsrA;Parent=BBa_C0040 BBa_K175045 . engineered_tag 1882 1906 . + 0 ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040 BBa_K175045 . CDS 1225 1841 . + 0 ID=annotation23329;Name=tetR;Parent=BBa_C0040 BBa_K175045 . terminator 1915 2043 . + 0 ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145201 BBa_K175045 . terminator 2003 2043 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K175045 . stem_loop 2010 2029 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K175045 . stop_codon 2036 2036 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K175045 . polyA_site 2030 2043 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K175045 . engineered_region 2003 2043 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K175045 . terminator 1915 1994 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K175045 . stem_loop 1926 1969 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K175045 . engineered_region 1915 1994 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K175045 . promoter 1161 1195 . + 0 ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K145201 BBa_K175045 . engineered_region 2052 3028 . + 0 ID=BBa_K145205;Name=BBa_K145205;Parent=BBa_K145280 BBa_K175045 . promoter 2052 2105 . + 0 ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K145205 BBa_K175045 . engineered_region 2052 2105 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_K175045 . promoter 2071 2076 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_K175045 . promoter 2094 2099 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_K175045 . non_covalent_binding_site 2052 2070 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_K175045 . non_covalent_binding_site 2077 2095 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 BBa_K175045 . ribosome_entry_site 2114 2126 . + 0 ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K145205 BBa_K175045 . engineered_region 2114 2126 . + 0 ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032 BBa_K175045 . ribosome_entry_site 2120 2123 . + 0 ID=annotation1710;Name=RBS;Parent=BBa_B0032 BBa_K175045 . ribosome_entry_site 2114 2126 . + 0 ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032 BBa_K175045 . terminator 2988 3028 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K145205 BBa_K175045 . stem_loop 2995 3014 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K175045 . stop_codon 3021 3021 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K175045 . polyA_site 3015 3028 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K175045 . engineered_region 2988 3028 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K175045 . terminator 2900 2979 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K145205 BBa_K175045 . stem_loop 2911 2954 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K175045 . engineered_region 2900 2979 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K175045 . engineered_region 2133 2891 . + 0 ID=BBa_K145015;Name=BBa_K145015;Parent=BBa_K145205 BBa_K175045 . CDS 2136 2848 . + 0 ID=annotation1969620;Name=cds;Parent=BBa_K145015 BBa_K175045 . tag 2849 2885 . + 0 ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015 BBa_K175045 . stop_codon 2889 2891 . + 0 ID=annotation1969618;Name=stop;Parent=BBa_K145015 BBa_K175045 . stop_codon 2886 2888 . + 0 ID=annotation1969617;Name=stop;Parent=BBa_K145015 BBa_K175045 . start_codon 2133 2135 . + 0 ID=annotation1969616;Name=start;Parent=BBa_K145015 BBa_K175045 . engineered_region 1 1114 . + 0 ID=BBa_K175041;Name=BBa_K175041 BBa_K175045 . terminator 1074 1114 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K175041 BBa_K175045 . stem_loop 1081 1100 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K175045 . stop_codon 1107 1107 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K175045 . polyA_site 1101 1114 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K175045 . engineered_region 1074 1114 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K175045 . terminator 986 1065 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K175041 BBa_K175045 . stem_loop 997 1040 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K175045 . engineered_region 986 1065 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K175045 . promoter 1 200 . + 0 ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K175041 BBa_K175045 . promoter 137 142 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_K175045 . CDS 1 88 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_K175045 . engineered_region 1 200 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_K175045 . promoter 161 166 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_K175045 . non_covalent_binding_site 166 200 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_K175045 . non_covalent_binding_site 89 126 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_K175045 . start_codon 173 173 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_K175045 . ribosome_entry_site 209 223 . + 0 ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K175041 BBa_K175045 . ribosome_entry_site 216 220 . + 0 ID=annotation1702;Name=RBS;Parent=BBa_B0030 BBa_K175045 . engineered_region 209 223 . + 0 ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030 BBa_K175045 . ribosome_entry_site 209 223 . + 0 ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030 BBa_K175045 . CDS 230 977 . + 0 ID=BBa_K142200;Name=BBa_K142200;Parent=BBa_K175041 BBa_K175045 . CDS 230 977 . + 0 ID=annotation1993900;Name=SceI;Parent=BBa_K142200 >BBa_K175045 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgcatca aaaaaaccaggtaatgaacctgggtccgaactctaaactgctgaaagaatacaaatcccagctgatcgaactgaacatc gaacagttcgaagcaggtatcggtctgatcctgggtgatgcttacatccgttctcgtgatgaaggtaaaacctactgta tgcagttcgagtggaaaaacaaagcatacatggaccacgtatgtctgctgtacgatcagtgggtactgtccccgccgca caaaaaagaacgtgttaaccacctgggtaacctggtaatcacctggggcgcccagactttcaaacaccaagccttcaac aaactggctaacctgttcatcgttaacaacaaaaaaaccatcccgaacaacctggttgaaaactacctgaccccgatgt ctctggcatactggttcatggatgatggtggtaaatgggattacaacaaaaactctaccaacaaatcgatcgtactgaa cacccagtctttcactttcgaagaagtagaatacctggttaagggtctgcgtaacaaattccaactgaactgttacgta aaaatcaacaaaaacaaaccgatcatctacatcgattctatgtcttacctgatcttctacaacctgatcaaaccgtacc tgatcccgcagatgatgtacaaactgccgaacactatctcctccgaaactttccttaagaggcccgctgcaaacgacga aaactacgctttagtagcttaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg cgtttatatactagagagttacgctagggataacagggtaatatagtactagagttgacagctagctcagtcctaggga ctatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattag agctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacatt gtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttt tgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatc gcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagccttttt atgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattg gaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaag ctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaa acaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctag tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcc ctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactaga tgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaatt ttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaacta cctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaac agcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaacta caagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaa gatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaaga atggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaa tactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatccc aacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactataca aaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaagg ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc ttcgggtgggcctttctgcgtttata