##gff-version 3
##sequence-region BBa_K175035 1 1409
BBa_K175035	.	terminator	301	380	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K175035	.	engineered_region	301	380	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K175035	.	stem_loop	312	355	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K175035	.	terminator	1369	1409	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K175035	.	polyA_site	1396	1409	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K175035	.	engineered_region	1369	1409	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K175035	.	stem_loop	1376	1395	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K175035	.	stop_codon	1402	1402	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K175035	.	mature_transcript_region	500	546	.	+	0	ID=BBa_K175031;Name=BBa_K175031
BBa_K175035	.	engineered_region	534	546	.	+	0	ID=annotation2027755;Name=Medium RBS B0032;Parent=BBa_K175031
BBa_K175035	.	promoter	438	491	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K175035	.	engineered_region	438	491	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K175035	.	promoter	457	462	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K175035	.	promoter	480	485	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K175035	.	non_covalent_binding_site	438	456	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K175035	.	non_covalent_binding_site	463	481	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K175035	.	CDS	553	1272	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K175035	.	CDS	553	1272	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K175035	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K175035	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K175035	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K175035	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K175035	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K175035	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K175035	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K175035	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K175035	.	mature_transcript_region	209	292	.	+	0	ID=BBa_K175032;Name=BBa_K175032
BBa_K175035	.	terminator	1281	1360	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K175035	.	engineered_region	1281	1360	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K175035	.	stem_loop	1292	1335	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K175035	.	terminator	389	429	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K175035	.	polyA_site	416	429	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K175035	.	engineered_region	389	429	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K175035	.	stem_loop	396	415	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K175035	.	stop_codon	422	422	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K175035
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagacccaaagtcctcacacaggaaagtggtt
aatgaaaattaacttactttcctgactgaaaaaagccgagttattaatccggctttactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtg
atagagatactgagcactactagaggtactatcctgtgtgaggactttgggtagatcactcacacaggaaagtactaga
tgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaatt
ttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaacta
cctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaac
agcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaacta
caagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaa
gatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaaga
atggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaa
tactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatccc
aacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactataca
aataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata