LOCUS       BBa_K1741007            1115 bp    DNA     linear       15-Dec-2025
DEFINITION  sfGFP under xylose promoter xylA (xylWT)
ACCESSION   BBa_K1741007
VERSION     BBa_K1741007.1  
FEATURES             Location/Qualifiers
     misc_feature    235..253
                     /label=CRP binding site
     misc_feature    254..268
                     /label=XylR binding site
     misc_feature    274..289
                     /label=XylR binding site
     misc_feature    290..294
                     /label=-35 signal
     misc_feature    313..318
                     /label=-10 signal
     misc_feature    324..365
                     /label=5'UTR
     misc_feature    366..395
                     /label=His-tag
     CDS             396..1109
                     /label=sfGFP
     misc_feature    1110..1115
                     /label=two consecutive STOP codons
ORIGIN
        1 ggtgtagggc cttctgtagt tagaggacag ttttaataag taacaatcac cgcgataaac
       61 gtaaccaatt tttagcaact aaacagggga aaacaattac agatttttat ctttcgatta
      121 cgatttttgg tttatttctt gatttatgac cgagatctta cttttgttgc gcaattgtac
      181 ttattgcatt tttctcttcg aggaattacc cagtttcatc attccatttt attttgcgag
      241 cgagcgcaca cttgtgaatt atctcaatag cagtgtgaaa taacataatt gagcaactga
      301 aagggagtgc ccaatattac gacatcatcc atcacccgcg gcattacctg attatggagt
      361 tcaatatggg cagcagccat catcatcatc atcatatgcg taaaggcgaa gagctgttca
      421 ctggtgtcgt ccctattctg gtggaactgg atggtgatgt caacggtcat aagttttccg
      481 tgcgtggcga gggtgaaggt gacgcaacta atggtaaact gacgctgaag ttcatctgta
      541 ctactggtaa actgccggta ccttggccga ctctggtaac gacgctgact tatggtgttc
      601 agtgctttgc tcgttatccg gaccatatga agcagcatga cttcttcaag tccgccatgc
      661 cggaaggcta tgtgcaggaa cgcacgattt cctttaagga tgacggcacg tacaaaacgc
      721 gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg cattgagctg aaaggcattg
      781 actttaaaga agacggcaat atcctgggcc ataagctgga atacaatttt aacagccaca
      841 atgtttacat caccgccgat aaacaaaaaa atggcattaa agcgaatttt aaaattcgcc
      901 acaacgtgga ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac actccaatcg
      961 gtgatggtcc tgttctgctg ccagacaatc actatctgag cacgcaaagc gttctgtcta
     1021 aagatccgaa cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc gcagcgggca
     1081 tcacgcatgg tatggatgaa ctgtacaaat aataa
//