##gff-version 3
##sequence-region BBa_K1688009 1 4977
BBa_K1688009	.	engineered_region	1	3810	.	+	0	ID=BBa_K1688005;Name=BBa_K1688005
BBa_K1688009	.	engineered_region	2011	3810	.	+	0	ID=BBa_K1688006;Name=BBa_K1688006;Parent=BBa_K1688005
BBa_K1688009	.	CDS	2029	3810	.	+	0	ID=BBa_K1688020;Name=BBa_K1688020;Parent=BBa_K1688006
BBa_K1688009	.	tag	3618	3809	.	+	0	ID=annotation2461027;Name=HlyA export tag;Parent=BBa_K1688020
BBa_K1688009	.	tag	2032	2048	.	+	0	ID=annotation2460174;Name=6xHis;Parent=BBa_K1688020
BBa_K1688009	.	ribosome_entry_site	2011	2022	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1688006
BBa_K1688009	.	conserved_region	2015	2018	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1688009	.	engineered_region	1	2002	.	+	0	ID=BBa_K1688012;Name=BBa_K1688012;Parent=BBa_K1688005
BBa_K1688009	.	promoter	1	1268	.	+	0	ID=BBa_J61051;Name=BBa_J61051;Parent=BBa_K1688012
BBa_K1688009	.	engineered_region	1277	2002	.	+	0	ID=BBa_K1688004;Name=BBa_K1688004;Parent=BBa_K1688012
BBa_K1688009	.	CDS	1295	2002	.	+	0	ID=BBa_K1688019;Name=BBa_K1688019;Parent=BBa_K1688004
BBa_K1688009	.	ribosome_entry_site	1277	1288	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1688004
BBa_K1688009	.	conserved_region	1281	1284	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1688009	.	engineered_region	3819	4977	.	+	0	ID=BBa_K1688022;Name=BBa_K1688022
BBa_K1688009	.	start_codon	3857	3859	.	+	0	ID=annotation2460971;Name=START;Parent=BBa_K1688022
BBa_K1688009	.	stop_codon	4975	4977	.	+	0	ID=annotation2461021;Name=STOP;Parent=BBa_K1688022
BBa_K1688009	.	null	3851	3856	.	+	0	ID=annotation2461088;Name=3A Scar;Parent=BBa_K1688022
BBa_K1688009	.	promoter	3819	3838	.	+	0	ID=annotation2460969;Name=T7 Promoter;Parent=BBa_K1688022
BBa_K1688009	.	tag	4792	4971	.	+	0	ID=annotation2461019;Name=HlyA C-tag;Parent=BBa_K1688022
BBa_K1688009	.	ribosome_entry_site	3839	3850	.	+	0	ID=annotation2461087;Name=RBS;Parent=BBa_K1688022
BBa_K1688009	.	sequence_alteration	4790	4791	.	+	0	ID=annotation2461022;Name=Frameshift insertion ;Parent=BBa_K1688022
BBa_K1688009	.	stop_codon	4972	4974	.	+	0	ID=annotation2461020;Name=STOP;Parent=BBa_K1688022
BBa_K1688009	.	CDS	3857	4789	.	+	0	ID=annotation2460970;Name=Dioxygenase;Parent=BBa_K1688022
>BBa_K1688009
ggccgctgcgatcccgcgaagaaccaaaaaagctcgacagagggcgcggtcattttaggtcgggcggatcggcgccgcc
ggctcggctggtgtgccgcacagcaccgcctacgtgagctgccagttgatgaacttcccccgttgccagctagggcgca
agcgggctgtataagatcactgcccatcacattgatcggctcggattttttctcaatccgtaaacaggtcaaacatcag
ttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacg
actgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagat
cggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgt
catgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccaga
gtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaaga
agccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcga
actgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatc
tcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgt
tgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgt
gcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccagg
ttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgca
gttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcga
gtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatagag
gatctactagagaaagaggagaaatactagatggttagcaagggtgaagaagtgattaaagaatttatgcgctttaaag
ttcgtatggaaggctcgatgaacggtcacgagttcgagatcgaaggtgagggtgagggtcgtccgtacgaaggcaccca
aaccgcgaaactgaaggttacgaagggtggcccgctgccgtttgcctgggacatcctgtccccgcagttcatgtacggt
agcaaggcgtatgtcaagcatccagcagacattccggactacaaaaagctgagcttcccggaaggtttcaaatgggagc
gtgtcatgaactttgaagatggcggcttggttaccgtgacccaagatagctctctgcaagacggcaccttgatctacaa
ggtgaagatgcgcggcacgaattttccgccggacggtccggtgatgcagaagaaaaccatgggttgggaagcaagcacg
gagcgtctgtacccgcgtgatggtgtcctgaaaggtgagattcaccaggcgctgaaactgaaagatggcggccattacc
tggtcgagttcaagaccatctatatggctaaaaagcctgtgcaactgccgggttactattatgttgataccaaactgga
cattacgagccataatgaagattatactatcgttgagcagtatgagcgtagcgagggtcgccaccacttgttcctgtac
ggtatggacgagctgtacaaataataatactagagaaagaggagaaatactagatgcatcatcatcatcatcatatgca
tcaacgtcgtgatttcttaaaatattccgtcgcgctgggtgtggcttcggctttgccgctgtggagccgcgcagtattt
gcggcagaacgcccaacgttaccgatccctgatttgctcacgaccgatgcccgtaatcgcattcagttaactattggcg
caggccagtccacctttggcgggaaaactgcaactacctggggctataacggcaatctgctggggccggcggtgaaatt
acagcgcggcaaagcggtaacggttgatatctacaaccaactgacggaagagacaacgttgcactggcacgggctggaa
gtaccgggtgaagtcgacggcggcccgcagggaattattccgccaggtggcaagcgctcggtgacgttgaacgttgatc
aacctgccgctacctgctggttccatccgcatcagcacggcaaaaccgggcgacaggtggcgatggggctggctgggct
ggtggtgattgaagatgacgagatcctgaaattaatgctgccaaaacagtggggtatcgatgatgttccggtgatcgtt
caggataagaaatttagcgccgacgggcagattgattatcaactggatgtgatgaccgccgccgtgggctggtttggcg
atacgttgctgaccaacggtgcaatctacccgcaacacgctgccccgcgtggttggctgcgcctgcgtttgctcaatgg
ctgtaatgcccgttcgctcaatttcgccaccagcgacaatcgcccgctgtatgtgattgccagcgacggtggtctgcta
cctgaaccagtgaaggtgagcgaactgccggtgctgatgggcgagcgttttgaagtgctggtggaggttaacgataaca
aaccctttgacctggtgacgctgccggtcagccagatggggatggcgattgcgccgtttgataagcctcatccggtaat
gcggattcagccgattgctattagtgcctccggtgctttgccagacacattaagtagcctgcctgcgttaccttcgctg
gaagggctgacggtacgcaagctgcaactctctatggacccgatgctcgatatgatggggatgcagatgctaatggaga
aatatggcgatcaggcgatggccgggatggatcacagccagatgatgggccatatggggcacggcaatatgaatcatat
gaaccacggcgggaagttcgatttccaccatgccaacaaaatcaacggtcaggcgtttgatatgaacaagccgatgttt
gcggcggcgaaagggcaatacgaacgttgggttatctctggcgtgggcgcaatgatgctgcatccgttccatatccacg
gcacgcagttccgtatcttgtcagaaaatggcaaaccgccagcggctcatcgcgcgggctggaaagataccgttaaggt
agaaggtaatgtcagcgaagtgctggtgaagtttaatcacgatgcaccgaaagaacatgcttatatggcgcactgccat
ctgctggagcatgaagatacggggctgatgttagggtttacggtaggctctggtacctctggcttagcctatggaagtc
agggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaag
aaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccaca
tcagcaggtacctaataatactagagtaatacgactcactatagggaaagaggagaaatactagatgaccgtgaaaatt
agccataccgcggatattcaggcgttttttaaccgcgtggcgggcctggatcatgcggaaggcaacccgcgctttaaac
agattattctgcgcgtgctacaggataccgcgcgcctgattgaagatctggaaattaccgaagatgaattttggcatgc
ggtggattatctgaaccgcctgggtggccgcaacgaagcgggcctgctggcggcgggcctgggcattgaacattttctg
gatctgctacaggatgcgaaagatgcggaagcgggcctgggcggcggcaccccgcgcaccattgaaggcccgctgtatg
tggcgggcgcgccgctggcgcagggcgaagcgcgcatggatgatggcaccgatccgggcgtggtgatgtttctacaggg
ccaggtgtttgatgcggatggcaaaccgctggcgggcgcgaccgtggatctgtggcatgcgaacacccagggcacctat
agctattttgatagcacccagagcgaatttaacctgcgccgccgcattattaccgatgcggaaggccgctatcgcgcgc
gcagcattgtgccgagcggctatggctgcgatccgcagggcccgacccaggaatgcctggatctgctgggccgccatgg
ccagcgcccggcgcatgtgcatttttttattagcgcgccgggccatcgccatctgaccacccagattaactttgcgggc
gataaatatctgtgggatgattttgcgtatgcgacccgcgatggcctgattggcgaactgcgctttgtggaagatgcgg
cggcggcgcgcgatcgcggcgtgcagggcgaacgctttgcggaactgagctttgattttcgcctacagggcgcgaaaag
cccggatgcggaagcgcgcagccatcgcccgcgcgcgctacaggaaggcagttagcctatggaagtcagggtgatctta
atccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttc
tttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataa