Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jens Sivk??r Pettersen
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-14 04:53:02
Sce VMA Intein with promoter
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1638036_sequence (Version 1) |
Description
This part consist of an intein from Saccharomyces cerevisiae VMA1 gene including a CBD-tag. This intein is a thiol-induced self-cleavable protein that enables the release of a C-terminal fused protein. The chitin-binding domain is an affinity tag that enables affinity purification on a chitin column. First the target protein fused to intein is loaded and washed on the chitin column. Using a thiol reagent, like dithiothreitol (DTT), an on-column cleavage is induced and the target protein is released [1].Notes
.Source
.| Access | Instance | Definition |
| public public public | BBa_R0011 BBa_K1638017 BBa_K1638034 | lacI+pL BBa_K1638017 BBa_K1638034 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0011 BBa_K1638017 BBa_K1638034 | 1,54 56,74 75,1608 | lacI+pL BBa_K1638017 BBa_K1638034 |