##gff-version 3
##sequence-region BBa_K1638030 1 1976
BBa_K1638030	.	engineered_region	1009	1976	.	+	0	ID=BBa_K1638013;Name=BBa_K1638013
BBa_K1638030	.	engineered_region	1009	1856	.	+	0	ID=BBa_K1638003;Name=BBa_K1638003;Parent=BBa_K1638013
BBa_K1638030	.	promoter	1009	1150	.	+	0	ID=BBa_K1638000;Name=BBa_K1638000;Parent=BBa_K1638003
BBa_K1638030	.	operator	1129	1150	.	+	0	ID=annotation2431868;Name=Lac operator;Parent=BBa_K1638000
BBa_K1638030	.	non_covalent_binding_site	1063	1078	.	+	0	ID=annotation2431869;Name=CAP binding;Parent=BBa_K1638000
BBa_K1638030	.	promoter	1096	1120	.	+	0	ID=annotation2431867;Name=Lac promoter;Parent=BBa_K1638000
BBa_K1638030	.	CDS	1170	1856	.	+	0	ID=BBa_K1638002;Name=BBa_K1638002;Parent=BBa_K1638003
BBa_K1638030	.	sequence_feature	1851	1856	.	+	0	ID=annotation2431871;Name=BamHI restriction site;Parent=BBa_K1638002
BBa_K1638030	.	ribosome_entry_site	1151	1169	.	+	0	ID=BBa_K1638001;Name=BBa_K1638001;Parent=BBa_K1638003
BBa_K1638030	.	sequence_feature	1159	1164	.	+	0	ID=annotation2431870;Name=Shine-Dalgarno;Parent=BBa_K1638001
BBa_K1638030	.	engineered_region	1857	1976	.	+	0	ID=BBa_S05273;Name=BBa_S05273;Parent=BBa_K1638013
BBa_K1638030	.	CDS	1881	1976	.	+	0	ID=BBa_K1638012;Name=BBa_K1638012;Parent=BBa_S05273
BBa_K1638030	.	polypeptide_domain	1857	1880	.	+	0	ID=BBa_K1638016;Name=BBa_K1638016;Parent=BBa_S05273
BBa_K1638030	.	engineered_region	1	1000	.	+	0	ID=BBa_K861173;Name=BBa_K861173
BBa_K1638030	.	promoter	1	131	.	+	0	ID=BBa_K118011;Name=BBa_K118011;Parent=BBa_K861173
BBa_K1638030	.	non_covalent_binding_site	1	42	.	+	0	ID=annotation1972435;Name=cAMP receptor protein binding site;Parent=BBa_K118011
BBa_K1638030	.	non_covalent_binding_site	101	131	.	+	0	ID=annotation1972436;Name=RNA polymerase binding site;Parent=BBa_K118011
BBa_K1638030	.	engineered_region	140	1000	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_K861173
BBa_K1638030	.	terminator	960	1000	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K1638030	.	stem_loop	967	986	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1638030	.	stop_codon	993	993	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1638030	.	engineered_region	960	1000	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1638030	.	polyA_site	987	1000	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1638030	.	ribosome_entry_site	140	151	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K1638030	.	conserved_region	144	147	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1638030	.	terminator	872	951	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K1638030	.	stem_loop	883	926	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1638030	.	engineered_region	872	951	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1638030	.	CDS	158	838	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K1638030	.	CDS	158	832	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1638030	.	engineered_tag	839	863	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
>BBa_K1638030
actcggttaacggagtgatcgagttaacattgttaagttaaatattggtttcaactccgatttacatggttgctgtgtt
gttaaattgtacaaagatgttatagaaacaaaatgtaacatctctatggacatactagagaaagaggagaaatactaga
tggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagtt
cgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccg
ctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcc
cggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttac
cgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgac
ggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaag
gtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaa
accggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgtt
gaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctggcacgacaggtttccc
gactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacacttta
tgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgcagcaat
cgcatcaggctggttacgcaaacgccgccgaccgggagtctggcatccccgcagccgtactcgatggcatcaaggccgt
ggcgaaggaaaaaaacgccacattgatgttccgcctggtcaacccccattccaccagcctgattgccgaaggggtggcc
accaaaggattgggcgtgcacgccaagtcgtccgattgggggttgcaggcgggctacattcccgtcaacccgaatcttt
ccaaactgttcggccgtgcgcccgaggtgatcgcgcgggccgacaacgacgtcaacagcagcctggcgcatggccatac
cgcggtcgacctgacgctgtcgaaagagcggcttgactatctgcggcaagcgggcctggtcaccggcatggccgatggc
gtggtcgcgagcaaccacgcaggctacgagcagttcgagtttcgcgtgaaggaaacctcggacgggcgctatgccgtgc
agtatcgccgcaagggcggcgacgatttcgaggcggtcaaggtgatcggcaatgccgccggtattccactgacggcgga
tatcgacatgttcgccattatgccgcatctgtccaacttccgcgactcggcgcgcagttcggtgaccagcggcgattcg
gtgaccgattacctggcgcgcacgcggcgggctggatccgaaaatttgtattttcaatctggtatgaaacagctggaag
acaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgt
t