##gff-version 3
##sequence-region BBa_K1631022 1 1330
BBa_K1631022	.	engineered_region	64	1330	.	+	0	ID=BBa_K1631001;Name=BBa_K1631001
BBa_K1631022	.	terminator	1202	1281	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K1631001
BBa_K1631022	.	stem_loop	1213	1256	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1631022	.	engineered_region	1202	1281	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1631022	.	ribosome_entry_site	420	431	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1631001
BBa_K1631022	.	conserved_region	424	427	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1631022	.	engineered_region	64	411	.	+	0	ID=BBa_K1631000;Name=BBa_K1631000;Parent=BBa_K1631001
BBa_K1631022	.	CDS	79	411	.	+	0	ID=annotation2452447;Name=Barnase catalitic domain ( I716211 );Parent=BBa_K1631000
BBa_K1631022	.	ribosome_entry_site	64	75	.	+	0	ID=annotation2455461;Name=BBa_B0034;Parent=BBa_K1631000
BBa_K1631022	.	start_codon	76	78	.	+	0	ID=annotation2452453;Name=ATG Start Codon;Parent=BBa_K1631000
BBa_K1631022	.	terminator	1290	1330	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K1631001
BBa_K1631022	.	stem_loop	1297	1316	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1631022	.	stop_codon	1323	1323	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1631022	.	polyA_site	1317	1330	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1631022	.	engineered_region	1290	1330	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1631022	.	CDS	438	1193	.	+	0	ID=BBa_K082003;Name=BBa_K082003;Parent=BBa_K1631001
BBa_K1631022	.	engineered_region	438	1157	.	+	0	ID=annotation1992959;Name=GFP;Parent=BBa_K082003
BBa_K1631022	.	tag	1158	1190	.	+	0	ID=annotation1993327;Name=LVA;Parent=BBa_K082003
BBa_K1631022	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K1631022	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1631022	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1631022	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1631022	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1631022	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
>BBa_K1631022
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaaatgg
cacaggttatcaacacgtttgacggggttgcggattatcttcagacatatcataagctacctgataattacattacaaa
atcagaagcacaagccctcggctgggtggcatcaaaagggaaccttgcagacgtcgctccggggaaaagcatcggcgga
gacatcttctcaaacagggaaggcaaactcccgggcaaaagcggacgaacatggcgtgaagcggatattaactatacat
caggcttcagaaattcagaccggattctttactcaagcgactggctgatttacaaaacaacggaccattatcagacctt
tacaaaaatcagataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcc
caattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacata
cggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggt
tatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggtt
atgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatac
ccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaac
tataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaaca
ttgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccaga
caaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagttt
gtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttag
tagcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata