LOCUS       BBa_K1618006            1903 bp    DNA     linear       11-Dec-2025
DEFINITION  pSB1C3-Pro-Rv3030
ACCESSION   BBa_K1618006
VERSION     BBa_K1618006.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..907
                     /label=BBa_E1010
     stem_loop       952..995
                     /label=stem_loop
     misc_feature    941..1020
                     /label=BBa_B0010
     terminator      941..1020
                     /label=BBa_B0010
     stem_loop       1036..1055
                     /label=T7 TE
     misc_feature    1062..1062
                     /label=stop
     polyA_site      1056..1069
                     /label=polya
     misc_feature    1029..1069
                     /label=BBa_B0012
     terminator      1029..1069
                     /label=BBa_B0012
     misc_feature    1..1069
                     /label=BBa_J04450
     CDS             1076..1903
                     /label=BBa_K1618002
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
      961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagatgtg
     1081 cgcctttgtg ccgcacgtgc ctcgccattc gcgcggagat aatccaccta gtgcgagtac
     1141 ggcttcgcct gcggttctga ctttgactgg agaacgtact attcccgatc tggacattga
     1201 aaactactgg tttcggcgcc accaagtagt gtaccagcgt ctggctccgc gttgcaccgc
     1261 acgtgatgtt ctggaagccg gttgcggtga aggttacgga gcggatctca tcgcttgcgt
     1321 tgcccggcaa gtgattgcgg tcgactatga tgaaactgcc gtggcacatg ttcgtagccg
     1381 ctatccacgc gtcgaggtga tgcaggcgaa cttagcagaa ctgccgctgc ctgatgcgtc
     1441 tgtagacgtc gttgtgaact ttcaagtcat tgaacacctg tgggaccagg cgcgtttcgt
     1501 ccgcgagtgc gcacgggtac tgcgtggctc gggcctgctc atggtgagta ccccgaatcg
     1561 cattacgttt tctccggggc gcgatacccc gattaatcca tttcataccc gcgaactgaa
     1621 tgcagatgaa ttaaccagcc tgctgattga tgccggtttt gtggatgtgg ccatgtgtgg
     1681 tctgtttcat ggcccgcgcc ttcgggatat ggatgcccgg catggaggca gcatcattga
     1741 cgcgcagatt atgcgtgctg tagcgggcgc gccttggccg ccggagttgg ccgcggatgt
     1801 agccgcagtc accacggcgg atttcgagat ggtagcggcg ggccatgacc gcgatattga
     1861 tgacagttta gacttaattg ccattgcggt tcgtccataa taa
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