##gff-version 3
##sequence-region BBa_K1585321 1 4996
BBa_K1585321	.	engineered_region	1	1317	.	+	0	ID=BBa_K1585312;Name=BBa_K1585312
BBa_K1585321	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1585312
BBa_K1585321	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1585321	.	CDS	19	1317	.	+	0	ID=BBa_K118016;Name=BBa_K118016;Parent=BBa_K1585312
BBa_K1585321	.	sequence_alteration	1024	1026	.	+	0	ID=annotation1978557;Name=G336D;Parent=BBa_K118016
BBa_K1585321	.	point_mutation	1086	1091	.	+	0	ID=annotation1978559;Name=EcoRI site removed;Parent=BBa_K118016
BBa_K1585321	.	point_mutation	589	594	.	+	0	ID=annotation1978558;Name=EcoRI site removed;Parent=BBa_K118016
BBa_K1585321	.	engineered_region	1326	2780	.	+	0	ID=BBa_K1585310;Name=BBa_K1585310
BBa_K1585321	.	ribosome_entry_site	1326	1337	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1585310
BBa_K1585321	.	conserved_region	1330	1333	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1585321	.	CDS	1344	2780	.	+	0	ID=BBa_K1585300;Name=BBa_K1585300;Parent=BBa_K1585310
BBa_K1585321	.	point_mutation	1916	1916	.	+	0	ID=annotation2436889;Name=Replaced C with T to remove EcoRI site;Parent=BBa_K1585300
BBa_K1585321	.	CDS	1344	2780	.	+	0	ID=annotation2436886;Name=glgA;Parent=BBa_K1585300
BBa_K1585321	.	point_mutation	1505	1505	.	+	0	ID=annotation2436887;Name=Replaced G with C to remove AviII site;Parent=BBa_K1585300
BBa_K1585321	.	point_mutation	2348	2348	.	+	0	ID=annotation2436891;Name=Replaced G with A to remove PstI site;Parent=BBa_K1585300
BBa_K1585321	.	point_mutation	2312	2312	.	+	0	ID=annotation2436890;Name=Replaced G with A to remove PvuII site;Parent=BBa_K1585300
BBa_K1585321	.	point_mutation	1748	1748	.	+	0	ID=annotation2436888;Name=Replaced G with C to remove AvrII site;Parent=BBa_K1585300
BBa_K1585321	.	point_mutation	2540	2540	.	+	0	ID=annotation2436892;Name=Replaced T with C to remove AgeI site;Parent=BBa_K1585300
BBa_K1585321	.	engineered_region	2789	4996	.	+	0	ID=BBa_K1585311;Name=BBa_K1585311
BBa_K1585321	.	ribosome_entry_site	2789	2800	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1585311
BBa_K1585321	.	conserved_region	2793	2796	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1585321	.	CDS	2807	4996	.	+	0	ID=BBa_K1585301;Name=BBa_K1585301;Parent=BBa_K1585311
>BBa_K1585321
aaagaggagaaatactagatggttagtttagagaagaacgatcacttaatgttggcgcgccagctgccattgaaatctg
ttgccctgatactggcgggaggacgtggtacccgcctgaaggatttaaccaataagcgagcaaaaccggccgtacactt
cggcggtaagttccgcattatcgactttgcgctgtctaactgcatcaactccgggatccgtcgtatgggcgtgatcacc
cagtaccagtcccacactctggtgcagcacattcagcgcggctggtcattcttcaatgaagaaatgaacgagtttgtcg
atctgctgccagcacagcagagaatgaaaggggaaaactggtatcgcggcaccgcagatgcggtcacccaaaacctcga
cattatccgccgttataaagcggaatacgtggtgatcctggcgggcgaccatatctacaagcaagactactcgcgtatg
cttatcgatcacgtcgaaaaaggcgcacgttgcaccgttgcttgtatgccagtaccgattgaagaagcctccgcatttg
gcgttatggcggttgatgagaacgataaaattatcgaatttgttgaaaaacctgctaacccgccgtcaatgccgaacga
tccgagcaaatctctggcgagtatgggtatctacgtctttgacgccgactatctgtatgaactgctggaagaagacgat
cgcgatgagaactccagccacgactttggcaaagatttgattcccaagatcaccgaagccggtctggcctatgcgcacc
cgttcccgctctcttgcgtacaatccgacccggatgccgagccgtactggcgcgatgtgggtacgctggaagcttactg
gaaagcgaacctcgatctggcctctgtggtgccggaactggatatgtacgatcgcaattggccaattcgcacctacaat
gaatcattaccgccagcgaaattcgtgcaggatcgctccggtagccacgggatgacccttaactcactggtttccgacg
gttgtgtgatctccggttcggtggtggtgcagtccgttctgttctcgcgcgttcgcgtgaactcattctgcaacattga
ttccgccgtattgttaccggaagtatgggtaggtcgctcgtgccgtctgcgccgctgcgtcatcgatcgtgcttgtgtt
attccggaaggcatggtgattggtgaaaacgcagaggaagatgcacgtcgtttctatcgttcagaagaaggcatcgtgc
tggtaacgcgcgaaatgctacggaagttagggcataaacaggagcgataataatactagagaaagaggagaaatactag
atgcaggttttacatgtatgttcagagatgttcccgctgcttaaaaccggcggtctggctgatgttattggggcattac
ccgcagcacaaatcgcagacggcgttgacgctcgcgtactgttgcctgcatttcccgatattcgccgtggcgtgaccga
tgcccaggtagtatcccgtcgtgataccttcgccggacatatcacgctgttgttcggtcattacaacggggttggcatt
tacctgattgacgcgccgcatctctatgatcgtccgggaagcccgtatcacgataccaacttatttgcctataccgaca
acgtattgcgttttgcgctgctggggtgggttggggcagaaatggccagcgggcttgacccattctggcgtcctgatgt
ggtgcatgcccacgactggcatgcaggccttgcgcctgcgtatctggcggcgcgcgggcgtccggcgaagtcggtgttt
actgtgcacaacctggcctatcaaggcatgttttatgcacatcacatgaatgacatccaattgccatggtcattcttta
atattcatgggctggaatttaacggacaaatctctttcctgaaggccggtctgtactatgccgatcacattacggcggt
cagtccaacctacgctcgcgagatcaccgaaccgcagtttgcctacggtatggaaggtctgttgcaacagcgtcaccgt
gaagggcgtctttccggcgtactgaacggcgtggacgagaaaatctggagtccagagacggacttactgttggcctcgc
gttacacccgcgatacgttggaagataaagcggaaaataagcgccagttacaaatcgcaatggggcttaaggttgacga
taaagtgccgctttttgcagtggtgagccgtctgaccagccagaaaggtctcgacctggtgctggaagccttaccgggt
cttctggagcagggcgggcaactggcgctactcggcgcgggcgatccggtgctgcaagaaggtttccttgcggcggcag
cggaataccccggtcaggtgggcgttcagattggctatcacgaagcattttcgcatcgcattatgggcggcgcggacgt
cattctggtgcccagccgttttgaaccgtgcggcttaacgcaactttatggattgaagtacggtacgctgccgttagtg
cggcgcaccggcgggcttgctgatacggtttctgactgttctcttgagaaccttgcagatggcgtcgccagtgggtttg
tctttgaagatagtaatgcctggtcgctgttacgggctattcgacgtgcttttgtactgtggtcccgtccttcactgtg
gcggtttgtgcaacgtcaggctatggcaatggattttagctggcaggtcgcggcgaagtcgtaccgtgagctttactat
cgcttgaaataataatactagagaaagaggagaaatactagatgtccgatcgtatcgatagagacgtgattaacgcgct
aattgcaggccattttgccgatcctttttccgtactgggaatgcataaaaccaccgcgggactggaagtccgtgccctt
ttacccgacgctaccgatgtgtgggtgattgaaccgaaaaccgggcgcaaactcgcaaaactggagtgtctcgactcac
ggggattctttagcggcgtcattccgcgacgtaagaattttttccgctatcagttggctgttgtctggcatggtcagca
aaacctgattgatgatccttaccgttttggtccgctaatccaggaaatggatgcctggctattatctgaaggtactcac
ctgcgcccgtatgaaaccttaggcgcgcatgcagatactatggatggcgtcacaggtacgcgtttctctgtctgggctc
caaacgcccgtcgggtctcggtggttgggcaattcaactactgggacggtcgccgtcacccgatgcgcctgcgtaaaga
gagcggcatctgggaactgtttatccctggggcgcataacggtcagctctataaatacgagatgattgatgccaatggc
aacttgcgtctgaagtccgacccttatgcctttgaagcgcaaatgcgcccggaaaccgcgtctcttatttgcgggctgc
cggaaaaggttgtacagactgaagaacgcaaaaaagcgaatcagtttgatgcgccaatctctatttatgaagttcacct
gggttcctggcgtcgccacaccgacaacaatttctggttgagctaccgcgagctggccgatcaactggtgccttatgct
aaatggatgggctttacccacctcgaactactgcccattaacgagcatcccttcgatggcagttggggttatcagccaa
ccggcctgtatgcgccaacccgccgttttggtactcgcgacgacttccgttatttcattgatgccgcacacgcggctgg
tctgaacgtgattctcgactgggtgccaggccacttcccgactgatgactttgcgcttgccgaatttgatggcacgaac
ttgtatgaacacagcgatccgcgtgaaggctatcatcaggactggaacacgctgatctacaactatggtcgccgtgaag
tcagtaacttcctcgtcggtaacgcgctttactggattgaacgttttggtattgatgcgctgcgcgtcgatgcggtggc
gtcaatgatttatcgcgactacagccgtaaagagggggagtggattccgaacgaatttggcgggcgcgagaatcttgaa
gcgattgaatttttgcgtaataccaaccgtattcttggtgagcaggtttccggtgcggtgacaatggctgaggagtcta
ccgatttccctggcgtttctcgtccgcaggatatgggcggtctgggcttctggtacaagtggaacctcggctggatgca
tgacaccctggactacatgaagctcgacccggtttatcgtcagtatcatcacgataaactgaccttcgggattctctac
aactacactgaaaacttcgtcctgccgttgtcgcatgatgaagtggtccacggtaaaaaatcgattctcgaccgcatgc
cgggcgacgcatggcagaaattcgcgaacctgcgcgcctactatggctggatgtgggcattcccgggcaagaaactact
gttcatgggtaacgaatttgcccagggccgcgagtggaaccatgacgccagcctcgactggcatctgttggaaggcggc
gataactggcaccacggtgtccagcgtctggtgcgcgatctgaacctcacctaccgccaccataaagcaatgcatgaac
tggattttgacccgtacggctttgaatggctggtggtggatgacaaagaacgctcggtgctgatctttgtgcgtcgcga
taaagagggtaacgaaatcatcgttgccagtaactttacgccggtaccgcgtcatgattatcgcttcggcataaaccag
ccgggcaaatggcgtgaaatcctcaataccgattccatgcactatcacggcagtaatgcaggcaatggcggcacggtac
acagcgatgagattgccagccacggtcgtcagcattcactaagcctgacgctaccaccgctggccactatctggctggt
tcgggaggcagaataataa