##gff-version 3
##sequence-region BBa_K1532004 1 1342
BBa_K1532004	.	ribosome_entry_site	227	241	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1532004	.	ribosome_entry_site	227	241	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1532004	.	engineered_region	227	241	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1532004	.	ribosome_entry_site	234	238	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1532004	.	terminator	1196	1324	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1532004	.	terminator	1196	1275	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1532004	.	engineered_region	1196	1275	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1532004	.	stem_loop	1207	1250	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1532004	.	terminator	1284	1324	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1532004	.	engineered_region	1284	1324	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1532004	.	polyA_site	1311	1324	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1532004	.	stop_codon	1317	1317	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1532004	.	stem_loop	1291	1310	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1532004	.	sequence_feature	1333	1342	.	+	0	ID=BBa_K1532002;Name=BBa_K1532002
BBa_K1532004	.	non_covalent_binding_site	1335	1340	.	+	0	ID=annotation2391757;Name=Kpn I cutting site;Parent=BBa_K1532002
BBa_K1532004	.	CDS	248	1187	.	+	0	ID=BBa_C0080;Name=BBa_C0080
BBa_K1532004	.	CDS	248	1123	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_K1532004	.	engineered_tag	1163	1187	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_K1532004	.	tag	1124	1156	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_K1532004	.	sequence_feature	1	10	.	+	0	ID=BBa_K1532002;Name=BBa_K1532002
BBa_K1532004	.	non_covalent_binding_site	3	8	.	+	0	ID=annotation2391757;Name=Kpn I cutting site;Parent=BBa_K1532002
BBa_K1532004	.	promoter	19	218	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1532004	.	start_codon	191	191	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1532004	.	non_covalent_binding_site	107	144	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1532004	.	engineered_region	19	218	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1532004	.	CDS	19	106	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1532004	.	promoter	179	184	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1532004	.	non_covalent_binding_site	184	218	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1532004	.	promoter	155	160	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
>BBa_K1532004
gcggtaccggtactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacag
gtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggcttta
cactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagagga
gaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggttta
acgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctca
ccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgcc
aggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcc
tactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgc
atttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatct
gcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgag
gcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgc
cgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagcca
ggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctat
ttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatg
tagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatc
actactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcggtaccgg