##gff-version 3 ##sequence-region BBa_K152007 1 6965 BBa_K152007 . engineered_region 937 1846 . + 0 ID=BBa_K152002;Name=BBa_K152002 BBa_K152007 . ribosome_entry_site 939 946 . + 0 ID=annotation1999565;Name=rbs;Parent=BBa_K152002 BBa_K152007 . CDS 954 1844 . + 0 ID=annotation1999566;Name=cds;Parent=BBa_K152002 BBa_K152007 . engineered_region 4914 6081 . + 0 ID=BBa_K152003;Name=BBa_K152003 BBa_K152007 . CDS 4931 6079 . + 0 ID=annotation1999564;Name=cds;Parent=BBa_K152003 BBa_K152007 . ribosome_entry_site 4917 4923 . + 0 ID=annotation1999563;Name=rbs;Parent=BBa_K152003 BBa_K152007 . engineered_region 1 928 . + 0 ID=BBa_K152001;Name=BBa_K152001 BBa_K152007 . ribosome_entry_site 3 10 . + 0 ID=annotation1999567;Name=rbs;Parent=BBa_K152001 BBa_K152007 . CDS 18 926 . + 0 ID=annotation1999568;Name=cds;Parent=BBa_K152001 BBa_K152007 . engineered_region 6090 6965 . + 0 ID=BBa_E0240;Name=BBa_E0240 BBa_K152007 . ribosome_entry_site 6090 6102 . + 0 ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240 BBa_K152007 . engineered_region 6090 6102 . + 0 ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032 BBa_K152007 . ribosome_entry_site 6090 6102 . + 0 ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032 BBa_K152007 . ribosome_entry_site 6096 6099 . + 0 ID=annotation1710;Name=RBS;Parent=BBa_B0032 BBa_K152007 . CDS 6109 6828 . + 0 ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240 BBa_K152007 . CDS 6109 6828 . + 0 ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040 BBa_K152007 . terminator 6925 6965 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240 BBa_K152007 . stem_loop 6932 6951 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K152007 . engineered_region 6925 6965 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K152007 . stop_codon 6958 6958 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K152007 . polyA_site 6952 6965 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K152007 . terminator 6837 6916 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240 BBa_K152007 . engineered_region 6837 6916 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K152007 . stem_loop 6848 6891 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K152007 . engineered_region 3361 4905 . + 0 ID=BBa_K152006;Name=BBa_K152006 BBa_K152007 . ribosome_entry_site 3372 3380 . + 0 ID=annotation1999571;Name=rbs;Parent=BBa_K152006 BBa_K152007 . engineered_region 1855 3352 . + 0 ID=BBa_K118005;Name=BBa_K118005 BBa_K152007 . ribosome_entry_site 1855 1864 . + 0 ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K118005 BBa_K152007 . sequence_feature 1855 1857 . + 0 ID=annotation1938045;Name=SacI;Parent=BBa_J15001 BBa_K152007 . ribosome_entry_site 1858 1864 . + 0 ID=annotation1938046;Name=rbs;Parent=BBa_J15001 BBa_K152007 . CDS 1871 3352 . + 0 ID=BBa_K118003;Name=BBa_K118003;Parent=BBa_K118005 BBa_K152007 . point_mutation 2348 2353 . + 0 ID=annotation1968244;Name=PstI site removed;Parent=BBa_K118003 BBa_K152007 . CDS 1871 3349 . + 0 ID=annotation1995702;Name=crtI coding sequence;Parent=BBa_K118003 BBa_K152007 . point_mutation 2759 2764 . + 0 ID=annotation1968245;Name=PstI site removed;Parent=BBa_K118003 >BBa_K152007 agaaggaggattaccatatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggc tgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggt gcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatg acggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatgga cgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgcc ttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaac tgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgc tgaagctattttgatgacgaatcactttaaaaccagcacgctgttctgtgcctccatgcagatggcctcgattgttgcg aatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatt tgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggccc gagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcac gccacccaacattttattcaggcgtggtttgacaaaaaactcgctgccgtcagttaacttactagagagaaggaggatt acaaaatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgat gctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgcctta caaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccgg cgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgc catggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggc ttgatgatggcgcaaatcatgggcgtacgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagt tgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatga aggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaa gcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcga agcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccac gcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccct gcgcatctctggcagcgcccgctctaacttactagagctcaaggaggtactagatgaaaccaactacggtaattggtgc aggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaa cccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtg ccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccg cctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaat ccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggta ctgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgttta cagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaat cccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggca ccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagcca tatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtca aatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaa ctaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggt ttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttat ctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgc atttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagca ttacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcc tatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaacca ttactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgac agcaggtttgatgctggaggatctgatataataatactagagagacaataagaaggagaaggcgaatggctgagatgaa aaacctgaaaattgaggtggtgcgctataacccgaaagtcgataccgcaccgcatagcgcattctatgaagtgccttat gacgcaactacctcattactggatgcgctgggctacatcaaagacaacctggcaccggacctgagctaccgctggtcct gccgtatggcgatttgtggttcctgcggcatgatggttaacaacgtgccaaaactggcatgtaaaaccttcctgcgtga ttacaccgacggtatgaaggttgaagcgttagctaacttcccgattgaacgcgatctggtggtcgatatgacccacttc atcgaaagtctggaagcgatcaaaccgtacatcatcggcaactcccgcaccgcggatcagggtactaacatccagaccc cggcgcagatggcgaagtatcaccagttctccggttgcatcaactgtggtttgtgctacgccgcgtgcccgcagtttgg cctgaacccagagttcatcggtccggctgccattacgctggcgcatcgttataacgaagatagccgcgaccacggtaag aaggagcgtatggcgcagttgaacagccagaacggcgtatggagctgtactttcgtgggctactgctccgaagtctgcc cgaaacacgtcgatccggctgcggccattcagcagggcaaagtagaaagttcgaaagactttcttatcgcgaccctgaa accacgctaaggagtgcaacatgacgactaaacgtaaaccgtatgtacggccaatgacgtccacctggtggaaaaaatt gccgttttatcgcttttacatgctgcgcgaaggcacggcggttccggctgtgtggttcagcattgaactgattttcggg ctgtttgccctgaaaaatggcccggaagcctgggcgggattcgtcgactttttacaaaacccggttatcgtgatcatta acctgatcactctggcggcagctctgctgcacaccaaaacctggtttgaactggcaccgaaagcggccaatatcattgt aaaagacgaaaaaatgggaccagagccaattatcaaaagtctctgggcggtaactgtggttgccaccatcgtaatcctg tttgttgccctgtactggtaaggagcctgagatgattaatccaaatccaaagcgttctgacgaaccggtattctggggc ctcttcggggccggtggtatgtggagcgccatcattgcgccggtgatgatcctgctggtgggtattctgctgccactgg ggttgtttccgggtgatgcgctgagctacgagcgcgttctggcgttcgcgcagagcttcattggtcgcgtattcctgtt cctgatgatcgttctgccgctgtggtgtggtttacaccgtatgcaccacgcgatgcacgatctgaaaatccacgtacct gcgggcaaatgggttttctacggtctggctgctatcctgacagttgtcacgctgattggtgtcgttacaatctaacgca tcgcacttactagagagaaggaggattacaaaatgcaaccgcattatgatctgattctcgtgggggctggactcgcgaa tggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggc gggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatc actggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcg tttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatct gttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactga gcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatgga tgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagac acgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggtt ggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggca gcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggtt gccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcg agcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcg ggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcgg ctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaactta ctagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaatta gatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctta aatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctt tgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaact atatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcg agttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgt atacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgtt caactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcca cacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggat tacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct ttctgcgtttata