##gff-version 3
##sequence-region BBa_K1475007 1 2270
BBa_K1475007	.	engineered_region	1393	2270	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K1475007	.	ribosome_entry_site	1393	1407	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K1475007	.	ribosome_entry_site	1400	1404	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1475007	.	engineered_region	1393	1407	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1475007	.	ribosome_entry_site	1393	1407	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1475007	.	terminator	2230	2270	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K1475007	.	stop_codon	2263	2263	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1475007	.	polyA_site	2257	2270	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1475007	.	stem_loop	2237	2256	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1475007	.	engineered_region	2230	2270	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1475007	.	CDS	1414	2133	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K1475007	.	CDS	1414	2133	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1475007	.	terminator	2142	2221	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K1475007	.	engineered_region	2142	2221	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1475007	.	stem_loop	2153	2196	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1475007	.	promoter	1185	1384	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1475007	.	non_covalent_binding_site	1273	1310	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1475007	.	non_covalent_binding_site	1350	1384	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1475007	.	promoter	1321	1326	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1475007	.	engineered_region	1185	1384	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1475007	.	promoter	1345	1350	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1475007	.	start_codon	1357	1357	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1475007	.	CDS	1185	1272	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1475007	.	engineered_region	1	1176	.	+	0	ID=BBa_K1475008;Name=BBa_K1475008
BBa_K1475007	.	engineered_region	1	50	.	+	0	ID=BBa_K1475900;Name=BBa_K1475900;Parent=BBa_K1475008
BBa_K1475007	.	promoter	1	35	.	+	0	ID=BBa_J23106;Name=BBa_J23106;Parent=BBa_K1475900
BBa_K1475007	.	ribosome_entry_site	36	50	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K1475900
BBa_K1475007	.	ribosome_entry_site	43	47	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1475007	.	engineered_region	36	50	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1475007	.	ribosome_entry_site	36	50	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1475007	.	engineered_region	57	1176	.	+	0	ID=BBa_K1475902;Name=BBa_K1475902;Parent=BBa_K1475008
BBa_K1475007	.	CDS	57	1142	.	+	0	ID=BBa_I732100;Name=BBa_I732100;Parent=BBa_K1475902
BBa_K1475007	.	terminator	1143	1176	.	+	0	ID=BBa_B1002;Name=BBa_B1002;Parent=BBa_K1475902
BBa_K1475007	.	engineered_region	1143	1176	.	+	0	ID=annotation1898412;Name=B1002;Parent=BBa_B1002
BBa_K1475007	.	polyA_site	1167	1173	.	+	0	ID=annotation1898414;Name=Poly A tail;Parent=BBa_B1002
BBa_K1475007	.	polyA_site	1146	1151	.	+	0	ID=annotation1898415;Name=Poly A tail;Parent=BBa_B1002
BBa_K1475007	.	stem_loop	1152	1167	.	+	0	ID=annotation1898413;Name=stem loop;Parent=BBa_B1002
>BBa_K1475007
tttacggctagctcagtcctaggtatagtgctagcattaaagaggagaaatactagatgaaaccagtaacgttatacga
tgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacg
cgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagt
cgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgc
cgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaat
cttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcct
gcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacgg
tacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtc
tcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcg
actggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgc
caacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtg
ggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggc
aaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggt
gaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggca
cgacaggtttcccgactggaaagcgggcagtaataacgcaaaaaaccccgcttcggcggggttttttcgctactagagc
aatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcg
ggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctc
gtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgcgtaaa
ggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtca
gtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttcc
atggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgac
tttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacac
gtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaa
cattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatc
aaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaa
ttggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaa
gagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
tactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg
aacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata