##gff-version 3
##sequence-region BBa_K1461236 1 3767
BBa_K1461236	.	engineered_region	2742	3767	.	+	0	ID=BBa_K1461224;Name=BBa_K1461224
BBa_K1461236	.	engineered_region	2950	3767	.	+	0	ID=BBa_K1461207;Name=BBa_K1461207;Parent=BBa_K1461224
BBa_K1461236	.	CDS	2968	3630	.	+	0	ID=BBa_K1461007;Name=BBa_K1461007;Parent=BBa_K1461207
BBa_K1461236	.	terminator	3639	3767	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461207
BBa_K1461236	.	terminator	3727	3767	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461236	.	polyA_site	3754	3767	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461236	.	engineered_region	3727	3767	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461236	.	stem_loop	3734	3753	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461236	.	stop_codon	3760	3760	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461236	.	terminator	3639	3718	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461236	.	stem_loop	3650	3693	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461236	.	engineered_region	3639	3718	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461236	.	ribosome_entry_site	2950	2961	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461207
BBa_K1461236	.	conserved_region	2954	2957	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461236	.	promoter	2742	2941	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1461224
BBa_K1461236	.	start_codon	2914	2914	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1461236	.	CDS	2742	2829	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1461236	.	promoter	2902	2907	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1461236	.	non_covalent_binding_site	2907	2941	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1461236	.	non_covalent_binding_site	2830	2867	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1461236	.	engineered_region	2742	2941	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1461236	.	promoter	2878	2883	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1461236	.	engineered_region	1	2733	.	+	0	ID=BBa_K1461234;Name=BBa_K1461234
BBa_K1461236	.	engineered_region	1	1873	.	+	0	ID=BBa_K1461226;Name=BBa_K1461226;Parent=BBa_K1461234
BBa_K1461236	.	engineered_region	1	902	.	+	0	ID=BBa_K1461211;Name=BBa_K1461211;Parent=BBa_K1461226
BBa_K1461236	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K1461211
BBa_K1461236	.	engineered_region	139	902	.	+	0	ID=BBa_K1461202;Name=BBa_K1461202;Parent=BBa_K1461211
BBa_K1461236	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461202
BBa_K1461236	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461236	.	CDS	157	765	.	+	0	ID=BBa_K1461005;Name=BBa_K1461005;Parent=BBa_K1461202
BBa_K1461236	.	terminator	774	902	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461202
BBa_K1461236	.	terminator	862	902	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461236	.	polyA_site	889	902	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461236	.	engineered_region	862	902	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461236	.	stem_loop	869	888	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461236	.	stop_codon	895	895	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461236	.	terminator	774	853	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461236	.	stem_loop	785	828	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461236	.	engineered_region	774	853	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461236	.	engineered_region	911	1873	.	+	0	ID=BBa_K1461220;Name=BBa_K1461220;Parent=BBa_K1461226
BBa_K1461236	.	ribosome_entry_site	999	1010	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461220
BBa_K1461236	.	conserved_region	1003	1006	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461236	.	CDS	1017	1736	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K1461220
BBa_K1461236	.	CDS	1017	1736	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1461236	.	promoter	911	990	.	+	0	ID=BBa_K1461002;Name=BBa_K1461002;Parent=BBa_K1461220
BBa_K1461236	.	terminator	1745	1873	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461220
BBa_K1461236	.	terminator	1833	1873	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461236	.	polyA_site	1860	1873	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461236	.	engineered_region	1833	1873	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461236	.	stem_loop	1840	1859	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461236	.	stop_codon	1866	1866	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461236	.	terminator	1745	1824	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461236	.	stem_loop	1756	1799	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461236	.	engineered_region	1745	1824	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461236	.	engineered_region	1882	2733	.	+	0	ID=BBa_K1461222;Name=BBa_K1461222;Parent=BBa_K1461234
BBa_K1461236	.	CDS	1988	2596	.	+	0	ID=BBa_K1461005;Name=BBa_K1461005;Parent=BBa_K1461222
BBa_K1461236	.	promoter	1882	1961	.	+	0	ID=BBa_K1461002;Name=BBa_K1461002;Parent=BBa_K1461222
BBa_K1461236	.	terminator	2605	2733	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461222
BBa_K1461236	.	terminator	2693	2733	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461236	.	polyA_site	2720	2733	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461236	.	engineered_region	2693	2733	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461236	.	stem_loop	2700	2719	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461236	.	stop_codon	2726	2726	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461236	.	terminator	2605	2684	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461236	.	stem_loop	2616	2659	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461236	.	engineered_region	2605	2684	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461236	.	ribosome_entry_site	1970	1981	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461222
BBa_K1461236	.	conserved_region	1974	1977	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1461236
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggatt
gatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgc
tgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggca
taaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgat
agcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatc
gcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagt
gctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaa
agctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagaggcctccacaccgctcgtcacatcctgtgatccactctt
catcccgctacgtaacacctctgcatcgcgaaccaaaaccagtactagagaaagaggagaaatactagatgcgtaaagg
agaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagt
ggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccat
ggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactt
tttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgt
gctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaaca
ttcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaa
agttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaatt
ggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaaga
gagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcctccacaccgctc
gtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcatcgcgaaccaaaaccagtactagagaaagag
gagaaatactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccacca
gccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattt
tgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctg
ctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgca
acctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctgga
agcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgacc
gatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgc
tgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccg
cgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaa
tgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagc
ggataacaatttcacacatactagagaaagaggagaaatactagatgggcccgctgcatcatccggatgatgcgaccct
gattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgcatctggaacgttgcgcagaatgc
cgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcattagccgtgtggtgccgctgaaaa
gccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtgatgcatgcgatgccggcagcgaa
ccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctgagcaccctgaaatggaaaaccctg
attccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgctgctgaaaattgcaccaggcgtga
gcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcgtatcatgatgtgcagggtgaatt
tggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgtatccggatcgcgaatgcatttgc
gtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgcagccgttttttggcatttaatact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata