##gff-version 3
##sequence-region BBa_K1461228 1 2576
BBa_K1461228	.	engineered_region	1782	2576	.	+	0	ID=BBa_K1461223;Name=BBa_K1461223
BBa_K1461228	.	engineered_region	1990	2576	.	+	0	ID=BBa_K1461206;Name=BBa_K1461206;Parent=BBa_K1461223
BBa_K1461228	.	ribosome_entry_site	1990	2001	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461206
BBa_K1461228	.	conserved_region	1994	1997	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461228	.	terminator	2448	2576	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461206
BBa_K1461228	.	terminator	2448	2527	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461228	.	stem_loop	2459	2502	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461228	.	engineered_region	2448	2527	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461228	.	terminator	2536	2576	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461228	.	polyA_site	2563	2576	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461228	.	stop_codon	2569	2569	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461228	.	engineered_region	2536	2576	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461228	.	stem_loop	2543	2562	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461228	.	CDS	2008	2439	.	+	0	ID=BBa_K1461006;Name=BBa_K1461006;Parent=BBa_K1461206
BBa_K1461228	.	promoter	1782	1981	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1461223
BBa_K1461228	.	engineered_region	1782	1981	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1461228	.	promoter	1918	1923	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1461228	.	non_covalent_binding_site	1947	1981	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1461228	.	non_covalent_binding_site	1870	1907	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1461228	.	CDS	1782	1869	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1461228	.	start_codon	1954	1954	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1461228	.	promoter	1942	1947	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1461228	.	CDS	895	1636	.	+	0	ID=BBa_K1461008;Name=BBa_K1461008
BBa_K1461228	.	promoter	789	868	.	+	0	ID=BBa_K1461001;Name=BBa_K1461001
BBa_K1461228	.	terminator	2448	2576	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1461228	.	terminator	2448	2527	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461228	.	stem_loop	2459	2502	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461228	.	engineered_region	2448	2527	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461228	.	terminator	2536	2576	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461228	.	polyA_site	2563	2576	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461228	.	stop_codon	2569	2569	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461228	.	engineered_region	2536	2576	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461228	.	stem_loop	2543	2562	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461228	.	engineered_region	1	780	.	+	0	ID=BBa_K1461210;Name=BBa_K1461210
BBa_K1461228	.	engineered_region	44	780	.	+	0	ID=BBa_K1461201;Name=BBa_K1461201;Parent=BBa_K1461210
BBa_K1461228	.	CDS	62	643	.	+	0	ID=BBa_K1461004;Name=BBa_K1461004;Parent=BBa_K1461201
BBa_K1461228	.	terminator	652	780	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461201
BBa_K1461228	.	terminator	652	731	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461228	.	stem_loop	663	706	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461228	.	engineered_region	652	731	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461228	.	terminator	740	780	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461228	.	polyA_site	767	780	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461228	.	stop_codon	773	773	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461228	.	engineered_region	740	780	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461228	.	stem_loop	747	766	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461228	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461201
BBa_K1461228	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461228	.	promoter	1	35	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K1461210
BBa_K1461228	.	terminator	652	780	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1461228	.	terminator	652	731	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461228	.	stem_loop	663	706	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461228	.	engineered_region	652	731	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461228	.	terminator	740	780	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461228	.	polyA_site	767	780	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461228	.	stop_codon	773	773	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461228	.	engineered_region	740	780	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461228	.	stem_loop	747	766	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461228	.	ribosome_entry_site	877	888	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1461228	.	conserved_region	881	884	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461228	.	terminator	1645	1773	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1461228	.	terminator	1645	1724	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461228	.	stem_loop	1656	1699	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461228	.	engineered_region	1645	1724	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461228	.	terminator	1733	1773	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461228	.	polyA_site	1760	1773	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461228	.	stop_codon	1766	1766	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461228	.	engineered_region	1733	1773	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461228	.	stem_loop	1740	1759	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_K1461228
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaacgaaaccgatccg
gatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcc
tgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttg
gaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctat
gatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgc
cggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcga
agcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctg
gaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtc
gcaaaccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggc
gcggataaaaatttcatttgcccgcgacggattccccgcccatctatcgttgaacccatcagctgcgttcatcagcgat
actagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaatta
gatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctta
aatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctt
tgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaact
atatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcg
agttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgt
atacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgtt
caactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcca
cacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggat
tacacatggcatggatgaactatacaaacgacgaaaactacgctgctgtctaataatactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccga
ttcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagct
cactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaattt
cacacatactagagaaagaggagaaatactagatgaccccggaacgttttgtgcatctggcggatgcgtatggtgcaga
tctgcaacgttggccgagcgcagaacgtgcagcagcacaggcactgctggattgcggcgatgcgcaggcagtggcagcg
ctgcgtcaggcacattggctggatagccagctggatcgttatcaggtgccggcaccgagcccggcactggcacagcgta
ttattgcggcagcacaacagccgggtgcaccgttttggagccgctatgcaggttggctggcaagcctgggttgggtggg
tgtgggcctgacaggcgtggcagcgggtatgctggcggtggcactgagcctgccattaagcaccagcgcagaagcactg
ccaagcgtgtttgatcagagcgatgcagaatttgtgctgagcattaacgcggaagaagcggaacagtaatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttata