LOCUS       BBa_K1461214             820 bp    DNA     linear       09-Dec-2025
DEFINITION  constitutive cr-ecf20 generator
ACCESSION   BBa_K1461214
VERSION     BBa_K1461214.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..95
                     /label=rbs
     RBS             44..95
                     /label=BBa_K1461000
     CDS             102..683
                     /label=BBa_K1461004
     stem_loop       703..746
                     /label=stem_loop
     misc_feature    692..771
                     /label=BBa_B0010
     terminator      692..771
                     /label=BBa_B0010
     stem_loop       787..806
                     /label=T7 TE
     misc_feature    813..813
                     /label=stop
     polyA_site      807..820
                     /label=polya
     misc_feature    780..820
                     /label=BBa_B0012
     terminator      780..820
                     /label=BBa_B0012
     terminator      692..820
                     /label=BBa_B0015
     misc_feature    44..820
                     /label=BBa_K1461203
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gaggaactct accattcacc
       61 tcttggattt gggtattaaa gaggagaaag gtacctacta gatgaacgaa accgatccgg
      121 atctggaact gctgaaacgt attggcaaca acgatgcaca ggcggtgaaa gaaatggtga
      181 cccgcaaact gccgcgcctg ctggcgctgg cgagccgtct gctgggcgat gcggatgaag
      241 cgcgcgatat tgcgcaggaa agctttctgc gcatttggaa acaggcagcg agctggcgca
      301 gcgaacaggc gcgctttgat acctggctgc atcgcgtggc gctgaacctg tgctatgatc
      361 gcctgcgccg tcgtaaagaa catgtgccgg tggatagcga acatgcgtgc gaagcgctgg
      421 atacccgccc ggcgccggat gaacagctgg aagcgagcgc gcagagccgt cgcatggcgc
      481 aggcactgga tcagctgccg gatcgtcagc gcgaagcgat tgtgctgcaa tattatcagg
      541 aactgagcaa caccgaagca gcggcactga tgcagattag cgtggaagcg ctggaaagcc
      601 tgctgagccg cgcacgtcgc aacctgcgca gccatctggc ggaagcgccg ggtgcggatc
      661 tgagcggccg tcgcaaaccg taatactaga gccaggcatc aaataaaacg aaaggctcag
      721 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      781 cacactggct caccttcggg tgggcctttc tgcgtttata
//