##gff-version 3 ##sequence-region BBa_K145280 1 1868 BBa_K145280 . engineered_region 892 1868 . + 0 ID=BBa_K145205;Name=BBa_K145205 BBa_K145280 . promoter 892 945 . + 0 ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K145205 BBa_K145280 . engineered_region 892 945 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_K145280 . non_covalent_binding_site 892 910 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_K145280 . non_covalent_binding_site 917 935 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 BBa_K145280 . promoter 934 939 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_K145280 . promoter 911 916 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_K145280 . ribosome_entry_site 954 966 . + 0 ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K145205 BBa_K145280 . engineered_region 954 966 . + 0 ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032 BBa_K145280 . ribosome_entry_site 954 966 . + 0 ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032 BBa_K145280 . ribosome_entry_site 960 963 . + 0 ID=annotation1710;Name=RBS;Parent=BBa_B0032 BBa_K145280 . terminator 1740 1819 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K145205 BBa_K145280 . stem_loop 1751 1794 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K145280 . engineered_region 1740 1819 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K145280 . terminator 1828 1868 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K145205 BBa_K145280 . polyA_site 1855 1868 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K145280 . engineered_region 1828 1868 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K145280 . stem_loop 1835 1854 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K145280 . stop_codon 1861 1861 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K145280 . engineered_region 973 1731 . + 0 ID=BBa_K145015;Name=BBa_K145015;Parent=BBa_K145205 BBa_K145280 . stop_codon 1729 1731 . + 0 ID=annotation1969618;Name=stop;Parent=BBa_K145015 BBa_K145280 . start_codon 973 975 . + 0 ID=annotation1969616;Name=start;Parent=BBa_K145015 BBa_K145280 . tag 1689 1725 . + 0 ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015 BBa_K145280 . CDS 976 1688 . + 0 ID=annotation1969620;Name=cds;Parent=BBa_K145015 BBa_K145280 . stop_codon 1726 1728 . + 0 ID=annotation1969617;Name=stop;Parent=BBa_K145015 BBa_K145280 . engineered_region 1 883 . + 0 ID=BBa_K145201;Name=BBa_K145201 BBa_K145280 . ribosome_entry_site 44 55 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K145201 BBa_K145280 . conserved_region 48 51 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K145280 . CDS 62 746 . + 0 ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K145201 BBa_K145280 . tag 682 715 . + 0 ID=annotation23330;Name=SsrA;Parent=BBa_C0040 BBa_K145280 . engineered_tag 722 746 . + 0 ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040 BBa_K145280 . CDS 65 681 . + 0 ID=annotation23329;Name=tetR;Parent=BBa_C0040 BBa_K145280 . promoter 1 35 . + 0 ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K145201 BBa_K145280 . terminator 755 883 . + 0 ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145201 BBa_K145280 . terminator 843 883 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K145280 . polyA_site 870 883 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K145280 . engineered_region 843 883 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K145280 . stem_loop 850 869 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K145280 . stop_codon 876 876 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K145280 . terminator 755 834 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K145280 . stem_loop 766 809 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K145280 . engineered_region 755 834 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 >BBa_K145280 ttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgtccagattagataaa agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc tttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactac tagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattag atggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaa atttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgcttt gcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaacta tatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgta tacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttc aactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccac acaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggatt acacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactaga gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata