##gff-version 3
##sequence-region BBa_K1442112 1 1683
BBa_K1442112	.	ribosome_entry_site	28	652	.	+	0	ID=BBa_K1442023;Name=BBa_K1442023
BBa_K1442112	.	ribosome_entry_site	36	646	.	+	0	ID=annotation2396242;Name=EMCV internal Ribosome Entry SIte ;Parent=BBa_K1442023
BBa_K1442112	.	sequence_feature	28	35	.	+	0	ID=annotation2396241;Name=AscI restriction site;Parent=BBa_K1442023
BBa_K1442112	.	sequence_feature	647	652	.	+	0	ID=annotation2396273;Name=BsrGI restriction site;Parent=BBa_K1442023
BBa_K1442112	.	terminator	1638	1683	.	+	0	ID=BBa_K1442301;Name=BBa_K1442301
BBa_K1442112	.	engineered_region	653	1524	.	+	0	ID=BBa_K1442106;Name=BBa_K1442106
BBa_K1442112	.	CDS	688	1470	.	+	0	ID=BBa_K1442308;Name=BBa_K1442308;Parent=BBa_K1442106
BBa_K1442112	.	polyA_site	1460	1470	.	+	0	ID=annotation2420100;Name=poly A tail ;Parent=BBa_K1442308
BBa_K1442112	.	sequence_feature	708	713	.	+	0	ID=annotation2420093;Name=BsrGI restriction site ;Parent=BBa_K1442308
BBa_K1442112	.	stop_codon	1428	1430	.	+	0	ID=annotation2420095;Name=Stop codon;Parent=BBa_K1442308
BBa_K1442112	.	sequence_feature	688	695	.	+	0	ID=annotation2420092;Name=AscI resctriction site;Parent=BBa_K1442308
BBa_K1442112	.	CDS	714	1433	.	+	0	ID=annotation2420094;Name=GFP codon optimised for humans;Parent=BBa_K1442308
BBa_K1442112	.	sequence_feature	1434	1441	.	+	0	ID=annotation2420097;Name=MauBI restriciton site ;Parent=BBa_K1442308
BBa_K1442112	.	sequence_feature	1454	1459	.	+	0	ID=annotation2420098;Name=MluI;Parent=BBa_K1442308
BBa_K1442112	.	stop_codon	1431	1433	.	+	0	ID=annotation2420096;Name=Stop codon;Parent=BBa_K1442308
BBa_K1442112	.	promoter	653	679	.	+	0	ID=BBa_K1442029;Name=BBa_K1442029;Parent=BBa_K1442106
BBa_K1442112	.	terminator	1479	1524	.	+	0	ID=BBa_K1442301;Name=BBa_K1442301;Parent=BBa_K1442106
BBa_K1442112	.	promoter	1525	1637	.	+	0	ID=BBa_K1442006;Name=BBa_K1442006
BBa_K1442112	.	sequence_feature	1525	1532	.	+	0	ID=annotation2417090;Name=MauBI restriction site ;Parent=BBa_K1442006
BBa_K1442112	.	non_covalent_binding_site	1533	1629	.	+	0	ID=annotation2417092;Name=Anti-theophylline aptazyme;Parent=BBa_K1442006
BBa_K1442112	.	sequence_feature	1630	1637	.	+	0	ID=annotation2417091;Name=MluI restriction site;Parent=BBa_K1442006
BBa_K1442112	.	promoter	1	27	.	+	0	ID=BBa_K1442029;Name=BBa_K1442029
>BBa_K1442112
gcgaaattaatacgactcactatagggggcgcgccagaccacaacggtttccctctagcgggatcaattccgcccctct
ccctccccccccggtaacgttactggccgaagccgcttggaataaggccggtgtgcgtttgtctatatgttattttcca
ccatattgccgtcttttggcaatgtgagggcccggaaacctggccctgtcttcttgacgagcattcctaggggtctttc
ccctctcgccaaaggaatgcaaggtctgttgaatgtcgtgaaggaagcagttcctctggaagcttcttgaagacaaaca
acgtctgtagcgaccctttgcaggcagcggaaccccccacctggcgacaggtgcctctgcggccaaaagccacgtgtat
aagatacacctgcaaaggcggcacaaccccagtgccacgttgtgagttggatagttgtggaaagagtcaaatggctctc
ctcaagcgtattcaacaaggggctgaaggatgcccagaaggtaccccattgtatgggatctgatctggggcctcggtgc
acatgctttacatgtgtttagtcgaggttaaaaaacgtctaggccccccgaaccacggggacgtggttttcctttgaaa
aacacgataatacctgtacagcgaaattaatacgactcactatagggtactagagggcgcgccatcgagttatgctgta
caatgcggaaaggggaggagctcttcacaggcgtggtgcccatactggtggagttggacggcgacgtgaacggtcacaa
gttctcagtctctggggagggagagggcgatgccacctatggcaagcttacactgaagttcatttgtacgaccggcaag
ctccctgtgccatggcccaccctggtcacaaccttcggctacggggtgcagtgctttgccagatatcccgaccacatga
agcagcatgacttctttaagagtgcaatgcccgaagggtatgtgcaggaacgaacgattttttttaaggacgacgggaa
ttacaaaacccgcgccgaggttaagttcgagggcgataccctggttaatcggatagagctcaaaggaatagattttaaa
gaggacggtaacattctgggccacaagctcgaatataactataattcacacaacgtatacatcatggctgacaagcaga
aaaacggcatcaaagttaactttaagatacgacacaacattgaagacgggtctgtgcagttggctgaccactaccagca
gaacacgccgatcggggatggaccggtgctgctgcctgacaaccactacctctctacccagtctgccctgtctaaggac
cctaacgaaaagcgggatcacatggtgcttcttgagttcgtcactgccgcaggaattacacacggtatggacgaactct
ataagtaataacgcgcgcgactgaatgcatgacgcgtaaaaaaaaaaatactagagtagcataaccccttggggcctct
aaacgggtcttgaggggttttttcgcgcgcgtctccttcggtacatccagctgatgagtcccaaataggacgaaataca
taccagccgaaaggcccttggcaggtgtcctggattccacgaaggagataaacgcgttagcataaccccttggggcctc
taaacgggtcttgaggggtttttt