LOCUS       BBa_K1442112            1683 bp    DNA     linear       10-Dec-2025
DEFINITION  Aptazyme with forward GFP
ACCESSION   BBa_K1442112
VERSION     BBa_K1442112.1  
FEATURES             Location/Qualifiers
     promoter        1..27
                     /label=BBa_K1442029
     misc_feature    28..35
                     /label=AscI restriction site
     RBS             36..646
                     /label=EMCV internal Ribosome Entry SIte 
     misc_feature    647..652
                     /label=BsrGI restriction site
     RBS             28..652
                     /label=BBa_K1442023
     promoter        653..679
                     /label=BBa_K1442029
     misc_feature    688..695
                     /label=AscI resctriction site
     misc_feature    708..713
                     /label=BsrGI restriction site 
     CDS             714..1433
                     /label=GFP codon optimised for humans
     misc_feature    1428..1430
                     /label=Stop codon
     misc_feature    1431..1433
                     /label=Stop codon
     misc_feature    1434..1441
                     /label=MauBI restriciton site 
     misc_feature    1454..1459
                     /label=MluI
     polyA_site      1460..1470
                     /label=poly A tail 
     CDS             688..1470
                     /label=BBa_K1442308
     terminator      1479..1524
                     /label=BBa_K1442301
     misc_feature    653..1524
                     /label=BBa_K1442106
     misc_feature    1525..1532
                     /label=MauBI restriction site 
     misc_feature    1630..1637
                     /label=MluI restriction site
     misc_feature    1533..1629
                     /label=Anti-theophylline aptazyme
     promoter        1525..1637
                     /label=BBa_K1442006
     terminator      1638..1683
                     /label=BBa_K1442301
ORIGIN
        1 gcgaaattaa tacgactcac tatagggggc gcgccagacc acaacggttt ccctctagcg
       61 ggatcaattc cgcccctctc cctccccccc cggtaacgtt actggccgaa gccgcttgga
      121 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
      181 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
      241 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
      301 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
      361 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
      421 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
      481 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
      541 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc
      601 ccgaaccacg gggacgtggt tttcctttga aaaacacgat aatacctgta cagcgaaatt
      661 aatacgactc actatagggt actagagggc gcgccatcga gttatgctgt acaatgcgga
      721 aaggggagga gctcttcaca ggcgtggtgc ccatactggt ggagttggac ggcgacgtga
      781 acggtcacaa gttctcagtc tctggggagg gagagggcga tgccacctat ggcaagctta
      841 cactgaagtt catttgtacg accggcaagc tccctgtgcc atggcccacc ctggtcacaa
      901 ccttcggcta cggggtgcag tgctttgcca gatatcccga ccacatgaag cagcatgact
      961 tctttaagag tgcaatgccc gaagggtatg tgcaggaacg aacgattttt tttaaggacg
     1021 acgggaatta caaaacccgc gccgaggtta agttcgaggg cgataccctg gttaatcgga
     1081 tagagctcaa aggaatagat tttaaagagg acggtaacat tctgggccac aagctcgaat
     1141 ataactataa ttcacacaac gtatacatca tggctgacaa gcagaaaaac ggcatcaaag
     1201 ttaactttaa gatacgacac aacattgaag acgggtctgt gcagttggct gaccactacc
     1261 agcagaacac gccgatcggg gatggaccgg tgctgctgcc tgacaaccac tacctctcta
     1321 cccagtctgc cctgtctaag gaccctaacg aaaagcggga tcacatggtg cttcttgagt
     1381 tcgtcactgc cgcaggaatt acacacggta tggacgaact ctataagtaa taacgcgcgc
     1441 gactgaatgc atgacgcgta aaaaaaaaaa tactagagta gcataacccc ttggggcctc
     1501 taaacgggtc ttgaggggtt ttttcgcgcg cgtctccttc ggtacatcca gctgatgagt
     1561 cccaaatagg acgaaataca taccagccga aaggcccttg gcaggtgtcc tggattccac
     1621 gaaggagata aacgcgttag cataacccct tggggcctct aaacgggtct tgaggggttt
     1681 ttt
//