##gff-version 3
##sequence-region BBa_K1423007 1 1782
BBa_K1423007	.	engineered_region	44	1782	.	+	0	ID=BBa_J45199;Name=BBa_J45199
BBa_K1423007	.	terminator	1742	1782	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J45199
BBa_K1423007	.	stem_loop	1749	1768	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1423007	.	polyA_site	1769	1782	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1423007	.	stop_codon	1775	1775	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1423007	.	engineered_region	1742	1782	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1423007	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J45199
BBa_K1423007	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1423007	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1423007	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1423007	.	CDS	65	1645	.	+	0	ID=BBa_J45014;Name=BBa_J45014;Parent=BBa_J45199
BBa_K1423007	.	point_mutation	478	478	.	+	0	ID=annotation1961305;Name=a->g to eliminate EcoRI site;Parent=BBa_J45014
BBa_K1423007	.	sequence_alteration	1212	1212	.	+	0	ID=annotation1961301;Name=t -> c;Parent=BBa_J45014
BBa_K1423007	.	stop_codon	1640	1645	.	+	0	ID=annotation1961302;Name=double TAA stop codon;Parent=BBa_J45014
BBa_K1423007	.	CDS	65	1645	.	+	0	ID=annotation1961300;Name=ATF1;Parent=BBa_J45014
BBa_K1423007	.	terminator	1654	1733	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J45199
BBa_K1423007	.	stem_loop	1665	1708	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1423007	.	engineered_region	1654	1733	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1423007	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
>BBa_K1423007
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgaatgaaatcgat
gagaaaaatcaggcccccgtgcaacaagaatgcctgaaagagatgattcagaatgggcatgctcggcgtatgggatctg
ttgaagatctgtatgttgctctcaacagacaaaacttatatcgaaacttctgcacatatggagaattgagtgattactg
tactagggatcagctcacattagctttgagggaaatctgcctgaaaaatccaactcttttacatattgttctaccaaca
agatggccaaatcatgaaaattattatcgcagttccgaatactattcacggccacatccagtgcatgattatatttcag
tattacaagaattgaaactgagtggtgtggttctcaatgaacaacctgagtacagtgcagtaatgaagcaaatattaga
agagttcaaaaatagtaagggttcctatactgcaaaaatttttaaacttactaccactttgactattccttactttgga
ccaacaggaccgagttggcggctaatttgtcttccagaagagcacacagaaaagtggaaaaaatttatctttgtatcta
atcattgcatgtctgatggtcggtcttcgatccacttttttcatgatttaagagacgaattaaataatattaaaactcc
accaaaaaaattagattacattttcaagtacgaggaggattaccaattattgaggaaacttccagaaccgatcgaaaag
gtgatagactttagaccaccgtacttgtttattccgaagtcacttctttcgggtttcatctacaatcatttgagatttt
cttcaaaaggtgtctgtatgagaatggatgatgtggaaaaaaccgatgatgttgtcaccgagatcatcaatatttcacc
aacagaatttcaagcgattaaagcaaatattaaatcaaatatccaaggtaagtgtactatcactccgtttttacatgtt
tgttggtttgtatctcttcataaatggggtaaatttttcaaaccattgaacttcgaatggcttacggatatttttatcc
ccgcagattgccgctcacaactaccagatgatgatgaaatgagacagatgtacagatatggcgctaacgttggatttat
tgacttcaccccctggataagcgaatctgacatgaatgataacaaagaaaatttttggccacttattgagcactaccat
gaagtaatttcggaagctttaagaaataaaaagcatctccatggcttagggttcaatatacaaggcttcgttcaaaaat
atgtgaacattgacaaggtaatgtgcgatcgtgccatcgggaaaagacgcggaggtacattgttaagcaatgtaggtct
gtttaatcagttagaggagcccgatgccaaatattctatatgcgatttggcatttggccaatttcaaggatcctggcac
caagcattttccttgggtgtttgttcgactaatgtaaaggggatgaatattgttgttgcttcaacaaagaatgttgttg
gtagtcaagaatctctcgaagagctttgctccatttacaaagctctccttttaggcccttaataatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata