##gff-version 3
##sequence-region BBa_K1407007 1 1817
BBa_K1407007	.	engineered_region	893	1680	.	+	0	ID=BBa_K575014;Name=BBa_K575014
BBa_K1407007	.	promoter	893	945	.	+	0	ID=BBa_R0071;Name=BBa_R0071;Parent=BBa_K575014
BBa_K1407007	.	promoter	934	939	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K1407007	.	non_covalent_binding_site	893	912	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K1407007	.	promoter	911	916	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K1407007	.	start_codon	945	945	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K1407007	.	ribosome_entry_site	954	968	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K575014
BBa_K1407007	.	engineered_region	954	968	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1407007	.	ribosome_entry_site	954	968	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1407007	.	ribosome_entry_site	961	965	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1407007	.	CDS	975	1680	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K575014
BBa_K1407007	.	CDS	975	1649	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1407007	.	engineered_tag	1656	1680	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1407007	.	terminator	1689	1817	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1407007	.	terminator	1777	1817	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1407007	.	engineered_region	1777	1817	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1407007	.	polyA_site	1804	1817	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1407007	.	stop_codon	1810	1810	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1407007	.	stem_loop	1784	1803	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1407007	.	terminator	1689	1768	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1407007	.	engineered_region	1689	1768	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1407007	.	stem_loop	1700	1743	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1407007	.	engineered_region	1	884	.	+	0	ID=BBa_I1466;Name=BBa_I1466
BBa_K1407007	.	terminator	844	884	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I1466
BBa_K1407007	.	engineered_region	844	884	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1407007	.	polyA_site	871	884	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1407007	.	stop_codon	877	877	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1407007	.	stem_loop	851	870	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1407007	.	CDS	19	747	.	+	0	ID=BBa_C0171;Name=BBa_C0171;Parent=BBa_I1466
BBa_K1407007	.	terminator	756	835	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I1466
BBa_K1407007	.	engineered_region	756	835	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1407007	.	stem_loop	767	810	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1407007	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I1466
BBa_K1407007	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1407007
aaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccga
tccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgt
gcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccag
atgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcc
tgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgc
gccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgc
ctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccgg
tctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcct
gagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggct
gccgcctacgccgcggcgctgggtctcatctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttatatactagagtcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctac
tagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtat
ggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgct
aaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaag
cttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttat
gaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaa
ctgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgta
tgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctga
agttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacc
tcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgct
gatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata