##gff-version 3
##sequence-region BBa_K1407002 1 1738
BBa_K1407002	.	engineered_region	1	942	.	+	0	ID=BBa_I0466;Name=BBa_I0466
BBa_K1407002	.	CDS	19	780	.	+	0	ID=BBa_C0071;Name=BBa_C0071;Parent=BBa_I0466
BBa_K1407002	.	CDS	19	741	.	+	0	ID=annotation301192;Name=rhlR;Parent=BBa_C0071
BBa_K1407002	.	tag	742	774	.	+	0	ID=annotation306523;Name=LVA;Parent=BBa_C0071
BBa_K1407002	.	engineered_tag	781	805	.	+	0	ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071
BBa_K1407002	.	terminator	902	942	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0466
BBa_K1407002	.	stop_codon	935	935	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1407002	.	polyA_site	929	942	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1407002	.	stem_loop	909	928	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1407002	.	engineered_region	902	942	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1407002	.	terminator	814	893	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0466
BBa_K1407002	.	engineered_region	814	893	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1407002	.	stem_loop	825	868	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1407002	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0466
BBa_K1407002	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1407002	.	engineered_region	951	1738	.	+	0	ID=BBa_K575014;Name=BBa_K575014
BBa_K1407002	.	promoter	951	1003	.	+	0	ID=BBa_R0071;Name=BBa_R0071;Parent=BBa_K575014
BBa_K1407002	.	non_covalent_binding_site	951	970	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K1407002	.	start_codon	1003	1003	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K1407002	.	promoter	969	974	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K1407002	.	promoter	992	997	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K1407002	.	ribosome_entry_site	1012	1026	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K575014
BBa_K1407002	.	ribosome_entry_site	1019	1023	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1407002	.	engineered_region	1012	1026	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1407002	.	ribosome_entry_site	1012	1026	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1407002	.	CDS	1033	1738	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K575014
BBa_K1407002	.	engineered_tag	1714	1738	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1407002	.	CDS	1033	1707	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
>BBa_K1407002
aaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccga
tccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgt
gcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccag
atgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcc
tgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgc
gccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgc
ctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccgg
tctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcct
gagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggct
gccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagt
gctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactag
agtcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagattaaagaggagaaat
actagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtca
cgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggt
ggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctg
acatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgt
tgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccg
tccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctc
tgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggc
taaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacacc
atcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc