##gff-version 3
##sequence-region BBa_K1362422 1 1083
BBa_K1362422	.	engineered_region	8	1076	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_K1362422	.	terminator	1036	1076	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1362422	.	engineered_region	1036	1076	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1362422	.	stop_codon	1069	1069	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1362422	.	polyA_site	1063	1076	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1362422	.	stem_loop	1043	1062	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1362422	.	promoter	8	207	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1362422	.	non_covalent_binding_site	96	133	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1362422	.	engineered_region	8	207	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1362422	.	non_covalent_binding_site	173	207	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1362422	.	start_codon	180	180	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1362422	.	promoter	144	149	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1362422	.	CDS	8	95	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1362422	.	promoter	168	173	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1362422	.	CDS	234	914	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1362422	.	CDS	234	908	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1362422	.	engineered_tag	915	939	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1362422	.	ribosome_entry_site	216	227	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1362422	.	conserved_region	220	223	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1362422	.	terminator	948	1027	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1362422	.	stem_loop	959	1002	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1362422	.	engineered_region	948	1027	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1362422	.	sequence_feature	1	7	.	+	0	ID=BBa_K1362423;Name=BBa_K1362423
BBa_K1362422	.	sequence_feature	1077	1083	.	+	0	ID=BBa_K1362424;Name=BBa_K1362424
>BBa_K1362422
agagacccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgact
ggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgct
tccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgg
cttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcga
aatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctg
ccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccgg
actacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgt
tacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggt
ccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtg
aaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaacc
ggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaa
cagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggtctca